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IRE1-mediated degradation of pre-miR-301a promotes apoptosis through upregulation of GADD45A
Cell Communication and Signaling volume 21, Article number: 322 (2023)
Abstract
The unfolded protein response is a survival signaling pathway that is induced during various types of ER stress. Here, we determine IRE1âs role in miRNA regulation during ER stress. During induction of ER stress in human bronchial epithelial cells, we utilized next generation sequencing to demonstrate that pre-miR-301a and pre-miR-106b were significantly increased in the presence of an IRE1 inhibitor. Conversely, using nuclear-cytosolic fractionation on ER stressed cells, we found that these pre-miRNAs were decreased in the nuclear fractions without the IRE1 inhibitor. We also found that miR-301a-3p targets the proapoptotic UPR factor growth arrest and DNA-damage-inducible alpha (GADD45A). Inhibiting miR-301a-3p levels or blocking its predicted miRNA binding site in GADD45Aâs 3â UTR with a target protector increased GADD45A mRNA expression. Furthermore, an elevation of XBP1s expression had no effect on GADD45A mRNA expression. We also demonstrate that the introduction of a target protector for the miR-301a-3p binding site in GADD45A mRNA during ER stress promoted cell death in the airway epithelial cells. In summary, these results indicate that IRE1âs endonuclease activity is a two-edged sword that can splice XBP1 mRNA to stabilize survival or degrade pre-miR-301a to elevate GADD45A mRNA expression to lead to apoptosis.
Video Abstract
Background
Endoplasmic reticulum (ER) homeostasis is dependent on the proper folding and maturation of secretory pathway proteins, on lipid biosynthesis, and on cellular calcium homeostasis. Disruption of the ER homeostasis, however, leads to a stress response called the unfolded protein response (UPR), a multifunctional signaling pathway [1,2,3]. The UPR serves primarily as a cellular adaptive mechanism that counteracts the stress-related deregulation of ER function and promotes cellular survival from both intrinsic and extrinsic insults [4, 5]. If the recovery mechanisms are ineffective, however, and the ER stress is maintained, the UPR can promote cell death [6,7,8,9,10].
The UPR is mediated by three proximal ER stress sensors that include inositol-requiring enzyme 1 alpha (IRE1), PKRâlike endoplasmic reticulum kinase (PERK), and activating transcription factor 6 (ATF6) [6,7,8,9,10]. Among them, IRE1 regulates the most phylogenetically conserved arm of the UPR that regulates the balance between cell survival and cell death [11,12,13]. IRE1âs unique feature is its UPR-related RNase activity that is responsible for the production of a potent transcription factor, X boxâbinding proteinâ1 (XBP1s). IRE1 regulates this activation through the nonconventional splicing of XBP1 mRNA and the degradation of a large pool of cytoplasmic mRNAs in the regulatedâIRE1 dependent decay (RIDD) pathway [9, 14]. The complex role of IRE1 in the regulation of mammalian UPR cannot be fully explained by IRE1âs one known mRNA target, X boxâbinding proteinâ1 (XBP1), or through its RIDD activity. Here, we focused on the role that IRE1 plays on pre-miRNA stability during the UPR.
Using next-generation sequencing (NGS) of immortalized human bronchial epithelial cells, 16HBE14o-, we identified three miRNAs, hsa-miR-301a-3p, hsa-miR-106b-5p, and hsa-miR-17-5p that had decreased levels of expression during the UPR. Our analyses demonstrated that all 3 of their pre-miRNA precursors were degraded by IRE1. Furthermore, we demonstrated that the IRE1-mediated downregulation of hsa-miR-301a-3p allowed for the accumulation of a proapoptotic UPR factor, growth arrest and DNA-damage-inducible alpha (GADD45A) that is known to contribute to cell fate decisions [15,16,17]. The studies presented here demonstrate that during UPR, IRE1 degrades pre-miR-301a and this enhances the expression of GADD45A, and subsequently drives the ER stressed cells towards apoptosis. This study demonstrates how inhibition of miRNA maturation by IRE1 can modulate cell fate decisions during the UPR.
Methods
Cell culture
Human bronchial epithelial 16HBE14o- cells (Sigma-Aldrich, Cat. #SCC150, Poznan, Poland) were obtained as previously described [15, 18, 19]. 16HBE14o- cells were cultured in minimum essential modified Eagleâs medium (Invitrogen, Carlsbad, CA, USA) with 10% FBS in a humidified incubator at 37 °C in 5% CO2 in 6-well plates and allowed to grow to 70â80% confluence prior to the start of the experiments. The inducible stable HeLa S3 XBP1s cell line containing the cDNA sequence of XBP1s (NM_001079539.1) was obtained as described previously [20]. This cell line was cultured in Minimum Essential Modified Eagleâs Medium with 2 mM glutamine, hygromycin B (300 ”g/ml) and 10% tetracycline free fetal bovine serum (Takara Bio, USA) in a humidified incubator at 37 °C in 5% CO2 in 6-well plates. For the XBP1s 24 h induction, doxycycline (400 ”g/ml, D3072 Millipore-Sigma) was added to culture media for 24 h. Cells were allowed to grow to 70â80% confluence before the start of the experiments. The Ambionâą PARISâą system was used for the isolation of both RNA and native protein from the same experimental sample (AM1921) and was used according to the manufactureâs protocol to obtain the nuclear and cytosolic fractions. The follow up isolation of RNA and small non-coding RNA was performed with miRNeasy kit (Qiagen).
Induction of ER stress and activation of the UPR
Pharmacological induction of ER stress and activation of the UPR were performed as previously described [15, 19, 21, 22]. Briefly, cells were treated with the compounds for the time periods specified: Tm (2.5 ”g·mLâ1; Sigma, T7765), Tg (50 nm; Sigma, T9033), calpain inhibitor I (ALLN; 100 ÎŒm; Abcam, ab146608, Cambridge, UK). CTRL cells were treated with vehicle control (CTRL), DMSO (<â0.5% v/v; Sigma, D2650). To verify IRE1 activity, cells were treated with 20 ”M 4”8C (an IRE1 inhibitor, Sigma-Aldrich, SML0949) dissolved in DMSO (Sigma-Aldrich, St. Louis, MI, USA) [20, 21, 23].
Isolation of RNA and small non-coding RNA
Total RNA (containing both mRNA and miRNA) was isolated using miRNeasy kit (Qiagen). RNA concentrations were calculated based on the absorbance at 260 nm. RNA samples were stored at -70 °C until use.
miRNA analogs and target protector transfections
Cells were seeded onto 6-well plates or 35-mm dishes and transfected at 70â80% confluence with Lipofectamine RNAiMax (Thermo Fisher Scientific) according to the manufacturerâs protocol. mirVana miRNA Mimics and mirVana miRNA Inhibitors (both from Thermo Fisher Scientific) were used at final concentrations of 30 and 150 nM, respectively. mirVana pre-miRs and inhibitors used in this study are as follows: pre-miR-106b (MH10067), pre-miR-301a (MH10978) and inhibitors: miR-106b-5p (PM10067) and miR-301a-3p (MC10978) and inhibitor (PM10978). As additional controls, mirVanaâą miRNA Mimic Negative Control #1 (4,464,058), and mirVanaâą miRNA Inhibitor Negative Control #1 (4,464,076) were used. The Ambion Silencer Negative Control No. 1 siRNA (4,390,843; Thermo Fisher Scientific) was used as well.
The target protector (TP) and the respective control (morpholino oligo that targets a human beta-globin intron mutation that causes beta-thalassemia, 5â -CCTCTTACCTCAGTTACAATTTATA-3â) were purchased from Gene Tools. LLC (Philomath, OR, USA) and directed against the hsa-miR-301a-3p binding site in GADD45A (5âČ-ACTTCAGTGCAATTTGGTTCAGTTA-3â) mRNA. TPs were used at a final concentration of 1 ”M. The transfected cells were cultured for 2 days before analysis.
Measurement of mRNA quantitative Real Time PCR (qRT-PCR)
We used TaqMan One-Step RT-PCR Master Mix Reagents (Applied Biosystems) as described previously [19, 24] using the manufacturerâs protocol (retrotranscription: 15 min, 48ÂșC). The relative expressions were calculated using the 2âÎÎCt method [25] with the RPLP0 (Hs00420898_gh) gene as reference genes for the mRNA and RNU44 (001094) or RNU6 (HS_RNU6-2_11) for the miRNA and pre-miRNA, respectively. TaqMan probes ids used were: BIP (Hs00607129_gH), GADD45A (Hs00169255_m1), MT-CYB (Hs02596867_s1), hsa-miR-106b (000442), hsa-miR-301a (000528), hsa-miR-17 (002308), MT-CYB (Hs02596867_s1), pre-miRNA-17 (HS_miR-17_1_PR, MP00001064), pre-miRNA-106b (HS_miR-106b_1_PR, MP00000147), pre-miRNA-301a (HS_miR-301a_1_PR, MP00001771), pri-miR-17 (Hs03295901_pri), XBP1(S) (Hs03929085_g1). EREG (Hs00914313_m1).
Measurement of RNA half-life
GADD45A, hsa-miR-301a-3p, hsa-miR-106b-5p, pre-miR-301a and pre-miR-106b RNA half-lives were measured as described in [22, 26] with the following modifications. Cells were grown on 35-mm plastic dishes to âŒ80% confluency. Parallel experiments were performed in cells exposed to 2.5 ”g/ml Tm, Tm and 20 ÎŒm 4”8C, or cultured in control conditions. Actinomycin D (5 ”g/ml; MilliporeSigma, Burlington, MA, USA) was added to stop transcription at the start of the experiment, and the RNA was isolated at the indicated time intervals using Qiagen miRNeasy. Actinomycin D was maintained throughout the experiments. Total RNA levels were measured by real-time PCR at each time point using TaqMan-based assays and normalized to endogenous 18 S rRNA levels (amplified using a standard primer set). RNA values for each time point were calculated from 3 individual samples generated in at least 2 independent experiments. Relative RNA levels were plotted as differences from RNA levels at the initial time point (tâ=â0). The RNA half-lives were calculated from the exponential decay using the trend line equation C/C0â=âeâkdt, where C and C0 are RNA amounts at time t and t0, respectively, and kd is the RNA decay constant as previously described [26, 27].
Western Blots
Cells were lysed in SDS lysis buffer (4% SDS, 20% glycerol, 125 mM Tris-HCl pHâ=â6.8) supplemented with protease inhibitors (cOmplete âą Mini (Roche)). The insoluble material was removed by centrifugation at 15,000 g for 15 min. Protein concentrations were determined by Bio-Radâą DC-Protein Assays using bovine serum albumin (BSA) as standard. Following the normalization of protein concentrations, the lysates were mixed with an equal volume of 2X Laemmli sample buffer and incubated for 5 min at 95 °C prior to separation by SDS PAGE on Criterion TGX stain-free 4â15% gradient gels (Bio-Rad). Following SDS-PAGE, the proteins were transferred to polyvinylidene fluoride membranes (300 mA 4 h at 4 °C). The membranes were then blocked with BSA (Sigma-Aldrich) dissolved in PBS/Tween-20 (3% BSA, 0.5% Tween-20 for 1â2 h), followed by immunoblotting with the primary antibody: rabbit anti-GADD45A (1:2000, D17E8; Cell signaling) and rabbit anti-XBP-1 (S) (1:1000, D2C1F, Cell Signaling). After the washing steps, the membranes were incubated with goat anti-rabbit IgG (Hâ+âL chains) HRP-conjugated secondary antibodies (Bio-Rad) and detected using SuperSignal West Pico ECL (Thermo Fisher Scientific). Densitometry was performed using Image Lab software v. 4.1 (Bio-Rad).
Real-time cell viability assay
For real-time monitoring of cell viability, we applied real-time and label-free holographic microscopy-based monitoring of cell death and viability using HoloMonitor M4Âź time-lapse cytometer (Phase Holographic Imaging PHI AB, Lund Sweden). Holographic microscopy was used to follow the optical thickness and irregularity of cells exposed for up to 24 h to Tm in the presence or absence of pre-miR-301a. The images from up to 5 independent optical fields were collected and analyzed according to manufacture instructions with HoloMonitorÂź App Suite software. Healthy cells are irregular in shape and thin, whereas dying cells are round and thick [28,29,30,31,32,33]. For all analysis, the same cells parameters qualification was applied.
Next-generation RNA sequencing analyses
The RNA isolation and analyses were performed in 16HBE14o- cells as previously described [19]. Briefly, following total RNA isolation, samples were validated with qRT-PCR for ER stress activation prior to further analysis. Following rRNA depletion, the remaining RNA fraction was used for library construction and subjected to 100-bp paired-end sequencing on an Illumina HiSeq 2000 instrument (San Diego, CA, USA). Sequencing reads were aligned to the human reference genome assembly (hg19) using TopHat [34]. Transcript assembly and estimation of the relative abundance and tests for differential expression were carried out with Cufflinks and Cuffdiff [35]. The resulting data were validated with qRT-PCR. The heat map generation and hierarchical clustering were performed with the Morpheus Web server (Morpheus, https://software.broadinstitute.org/morpheus).
Small RNA sequencing libraries were prepared using QIAseq miRNA library kit (Qiagen) following the manufacturerâs instructions and followed by sequencing on an Illumina NextSeq instrument. Using Qiagenâs Gene Globe Software, sequencing reads were aligned to the human reference genome assembly (hg19) followed by transcript assembly and estimation of the relative abundances. The analysis of the differential expression of small RNAs between control and experimental samples were performed with geNorm [36] in the Gene Globe Software.
Statistical analysis
Results were expressed as a meanâ±âstandard deviation. Statistical significance was determined using the studentâs t-test with Pâ<â0.05 considered significant. The correlation was accessed via the Pearson product-moment correlation method.
Results
To determine IRE1âs role as a direct miRNA regulator during ER stress, we followed next generation sequencing changes in miRNA levels in 16HBE14o- cells that were exposed for 6 h to ER stress (induced by ALLN or tunicamycin (Tm)) in the presence of an IRE1 specific inhibitor, 4”8C [37]. In previous studies, we used a 6-hour time point as putative transitional time point for the cell fate decision process [15, 19]. We focused this current analysis on miRNAs that were downregulated in both ER stress models, and that their sequence contained the consensus IRE1 target sequence (UGCA) [38]. These two criteria would make them potentially prone to IRE1 endoribonuclease activity. In the case of ALLN treated cells, all of the selected miRNAs were induced when IRE1 activity was impaired. In Tm-treated cells, however, their miRNA levels were more resistant to UPR-related reduction and were only slightly reduced or unaffected (Fig. 1A). Furthermore, as shown in Supplemental Fig. 1A, IRE1 inhibition in tunicamycin-treated cells resulted in elevated levels of 12 miRNAs, whereas the levels of 51 were reduced. Interestingly, the presence of 4”8C during ALLN treatment increased the expression of 162 miRNAs and reduced levels of 964 others when compared to the âno stressâ exposed cells in the presence of IRE1 inhibitor. The observed discrepancies in the miRNA profiles between these two models resulted presumably from the different mechanisms of action of the two stressors, including inhibitory effects of ALLN on the IRE1 branch [15, 21]. Consequently, we limited the use of this NGS data to support initial selection of IRE1-dependent miRNAs and focused on miRNAs significantly increased in Tm treated cells in the presence of IRE1 inhibitor (Supplemental Fig. 1B). These miRNAs were verified with qPCR, and a positive control that was previously reported as an IRE1-degraded miRNA, miR-17-5p, was used as well [39].
As shown in Fig. 1A and B, the initial selection was limited to 5 miRNAs that were reduced during ER stress and contained an IRE1 target sequence (bolded) as well as 2 miRNAs that were previously reported to be IRE1-dependent ones [39]. Given the nonspecific effects of ALLN on IRE1 branch studies, we utilized a non-competitive inhibitor of the sarco-endoplasmic reticulum Ca ATPase, thapsigargin (Tg), as the second ER stress model for these studies. Consequently, based on the results of the independent, qPCR-based validation of the results from these two ER stress models (Tm and Tg), we selected hsa-miR-301a-3p and hsa-miR-106b-5p as potentially degraded by IRE1, and hsa-miR-17-5p as a previously identified target of IRE1 [39]. Importantly, all three of the selected miRNAs contained IRE1 target sequence in their pre-miRNA sequences (Fig. 1C). Next, we followed the selected miRNAs expression profiles during ER stress. As shown in Fig. 1D and E, hsa-miR-301a-3p was reduced in cells exposed to either Tm or Tg for 6 and 9 h, whereas reduction of hsa-miR-106b-5p was noted after 6- and 12-hours exposure. The putative control, hsa-miR-17-5p, was downregulated in both Tm and Tg treated cells after 4-, 6- and 9-hour exposure (Fig. 1F). Notably, all tested miRNAs were reduced by about half and these reductions were negatively correlated with XBP1s transcript accumulation (Fig. 1G) as a consequence of IRE1 activity. XBP1s mRNA began to accumulate after 4 h of exposure to ER stressors and reached a maximum after 9 h and remain elevated at 12 h.
Inhibition of IRE1 activity with 4”8C during ER stress not only prevented XBP1s mRNA and protein accumulation (Fig. 2A and B), but it also restored the expression of hsa-miR-17-5p, hsa-miR-301a-3p, and hsa-miR-106b-5p, in a stressor-independent manner (Fig. 2C, D, and E). Furthermore, the expression of miRNA biogenesis components (AGO2, DICER1 and DROSHA) remained unaffected up to 12 h of exposure to Tm-induced ER stress (Fig. 2F) in good agreement with previous reports [40]. These data suggest that UPR-related reduction of these miRNAs is IRE1-dependent. Since the consensus sequence that targets selected miRNAs to the IRE1 endoribonuclease activity was present not only in mature selected miRNAs, but also in their respective pre-miRNAs, we tested if the precursors could be an IRE1 substrates. A complication is that pre-miRNAs are the result of pri-miRNA maturation and the pre-miRNAs are exported from nucleus to cytosol to be further processed to mature miRNAs [41]. Furthermore, given that the nuclear envelope is continuous with ER membranes [42], IRE1 could process pre-miRNAs both in nucleus and cytosol. Hence, to test if IRE1 was degrading pre-miR-17, pre-miR-301a and pre-miR-106b, we compared levels of these precursors in nuclear and cytosolic fractions from cells exposed to ER stress with the pre-miRNAs obtained from cells treated with both ER stressors and the IRE1 inhibitor. Pri-miR-17, found only in the nucleus, was used as a quality control for the nuclear fraction, and mitochondrial encoded cytochrome b (CYTB) mRNA was used for the cytosolic control (Fig. 3A and B).
The pri-miR-17 was almost undetectable in the cytosolic fractions as expected. Despite the significant enrichment of CYTB in cytosol, much smaller amounts were also noted in nuclear fractions, presumably due to the presence of intimate contacts of mitochondria with nuclear envelope that have been proposed to be the gateway for mRNAs [43]. As shown in Fig. 3C, the expression of pri-miR-17 was induced by ER stress and was not affected by the IRE1 inhibitor. In contrast, as shown in Fig. 3D and E, pre-miR-17 levels were significantly increased in both nuclear and cytosolic fractions from the cells treated with ER stressors in the presence of 4”8C. Notably, the majority of pre-miR-17 was detected in nuclear fractions (Fig. 3D), which is in agreement with previous reports indicating that the majority of pre-miRNA is concentrated in the nucleus [44].
A similar pre-miRNA distribution was observed for both pre-miR-301a and pre-miR-106b that accumulated in the nucleus (Fig. 4). Upon IRE1 inhibition, pre-miR-301a levels were significantly increased in both fractions, independent of ER stressor (Fig. 4A and B). The pre-miR-106b levels were increased in nuclear fraction from the cells treated with Tm and Tg in the presence of 4”8C (Fig. 4C). This increase, however, was only found in the cytosolic fraction of Tm treated cells (Fig. 4D). The lack of a 4”8C effect in Tg treated cells in the cytosol could be a consequence of different ER stress models and the related UPR dynamics. Notably, as previously shown in Fig. 1E, IRE1 inhibition increased mature hsa-miR-106b-5p levels in both Tm and Tg treated cells, so the lack of an effect for the pre-miR is unclear. To get better understanding of IRE1-mediated degradation of these miRs and pre-miRs, we compared their stability under normal conditions, and during ER stress with and without IRE1 inhibition (Fig. 5). As shown in Fig. 5A and B, both ER stress and IRE1 inhibition did not dramatically affect the mature hsa-miR-301a-3p nor hsa-miR-106b-5p. Although, in Tm-treated cells, the half-life of both of these miRNAs were about 1 h shorter than in control conditions, and these molecules remained very stable with half-lives of about 10 h, which is in good agreement with previous reports [45]. Importantly, the stability of pre-miRs in the nucleus also remained unaffected (Fig. 5B and C). In contrast, their half-life in cytosol was significantly reduced during the Tm treatment. The pre-miR-301a was reduced from 3.55 h to 2.43 h and pre-miR-106b was reduced from 3.05 h to 1.91 h. Furthermore, the stabilities of pre-miRNA-106b and pre-miR-301a were completely restored to the values observed in the no stress conditions when IRE1 was inhibited (Fig. 5D and E).
At this point, the data supported the hypothesis that during UPR, IRE1 is reducing hsa-miR-301a-3p and hsa-miR-106b-5p via degradation of the respective pre-miRNAs in the cytosol, whereas the corresponding small differences in mature miRNA stability are the consequence of reduced pre-miRNA levels in cytosol. Although we blocked the pri-miRNA transcription, the pre-miRNA processing remained active.
Next, we asked what the functional consequence of this IRE1-dependent regulation of miRNA expression during the UPR was. Our goal was to establish direct targets for both hsa-miR-106b-5p and hsa-miR-301a-3p. To do so, we analyzed our previous transcriptomic data obtained from 16HBE14o- cells exposed to Tm for 2-, 6- and 9-hours [15] in order to find transcripts that were significantly induced during ER stress and contained the conserved binding sited for these miRNAs. This approach resulted in selection of epiregulin (EREG) as a potential target of hsa-miR-106b-5p. Although this transcript was induced by ER stress (Supplemental Fig. 1C), its levels remained unaffected when IRE1 was inhibited (Supplemental Fig. 1D), and therefore we next analyzed hsa-miR-301-3p.
In this case, we focused on growth arrest and DNA damage inducible alpha (GADD45A) as a potential target of hsa-miR-301a-3p. The 3â UTR region of GADD45A contains a 7-mer binding sequence for miR-301a-3p (Fig. 6A). Notably, both the binding sequence and the miRNAsâ seed sequence are strongly conserved among different species (Fig. 6B and C). GADD45A expression was induced by both Tm and Tg (Fig. 6D), and IRE1 inhibition resulted in a significant reduction of this transcript levels (Fig. 6E). Furthermore, when 4”8C was added to 16HBE14o- cells exposed to Tm and Tg for 9 h, when the maximum of GADD45A induction was observed, the levels of this transcript were dramatically reduced as well (Supplemental Fig. 1E).
Using a stable XBP1s-inducible cell line [20], we tested if GADD45A increased during the induction of XBP1s expression. As shown in Fig. 7A and B, GADD45A expression was not affected by the increase in XBP1s mRNA. Next, we asked if restoring hsa-miR-301a-3p levels during ER stress with its precursor (premiR) affected GADD45A expression levels. We utilized pre-miR-301a to limit the amount of mature miRNA and found that even under these conditions, the resulting hsa-miR-301a-3p levels were elevated by 400-fold in control conditions and by 200-fold during Tm treatment (Fig. 7C).
To demonstrate a direct interaction between miR-301a-3p and GADD45A mRNA, we used a specific target protector (Tp, target mask) molecule [46]. The target protector is a morpholino uniquely complementary to hsa-miR-301a-3p target sequence in the GADD45A 3âUTR, and interferes with a single miRNA-mRNA pair by binding specifically to the miRNA target sequence in the 3âČ UTR [46]. As shown in Fig. 7D, overexpression of pre-miR-301a in 16HBE14o- cells resulted in a significant reduction of GADD45A mRNA in both Tm and Tg treated cells. Furthermore, the Tp reversed the inhibitory effect of hsa-miR-301a-3p on GADD45A mRNA levels in ER stressed cells. The Tp was also effective in elevating GADD45A expression in the absence of pre-miR-301a transfection, in both unstressed and Tm and Tg treated cells (Fig. 7E). Since this data suggested that despite the IRE1-dependent degradation, the remaining levels of hsa-miR-301a-3p were still effectively modulating or buffering GADD45A expression, we tested if antagomiR (anti-miR-301a-3p) will allow further increase of this transcript in ER stressed cells. As shown in Fig. 7F, antagomiR transfection resulted in elevated GADD45A mRNA levels in both Tm and Tg treated cells, lending further support to the regulation of GADD45A mRNA by miR-301a-3p. Finally, we measured GADD45A mRNA stability in no stress conditions and during Tm induced ER stress in the presence of IRE1 inhibitor or with the addition of pre-miR-301a (Fig. 7G). The GADD45A mRNA was stabilized during the UPR, and the mRNA half-life increased from about 1 to 1.91 h. IRE1 inhibition restored this transcript stability to control levels (half-life of 1.1 h) during Tm treatment. These results are in good agreement with the previous report of a 45 min GADD45A mRNA half-life [47]. Furthermore, significant GADD45A mRNA destabilization was also observed when the cells were transfected with pre-miR-301a (half-life of 1.4 h) during Tm treatment.
Next, we tested if IRE1 inhibition also resulted in increased GADD45α protein levels in Tm treated cells. As shown in Fig. 8A and B, consistent with our mRNA results, GADD45α protein levels in the presence of 4”8C were significantly decreased. Furthermore, increasing hsa-miR-301a-3p levels with its pre-miR resulted in a dramatic reduction of GADD45α expression in Tm treated cells as well (Fig. 8C and D). A similar negative effect of hsa-miR-301a-3p on GADD45α was also observed when cells were exposed to Tg (Supplemental Fig. 2AB). Furthermore, silencing ERN1 during ER stress [48] not only led to the reduction of XBP1s mRNA levels, but also resulted in increased expression of all tested miRNAs (hsa-miR-17-5p, hsa-miR-301a-3p and hsa-miR-106b-5p) and reduced GADD45A mRNA levels (Supplemental Fig. 3).
Taken together, the data clearly demonstrates that hsa-miR-301a-3p affects not only GADD45A mRNA expression, but also GADD45α protein expression during ER stress.
This data suggested that IRE1âs reduction of hsa-miR-301a-3p levels and the corresponding increases GADD45A expression could contribute to cell death decisions during ER stress. To test this, we performed real time and label free holographic microscopy-based monitoring of cell death and viability using a HoloMonitorÂź time-lapse cytometer. Holographic microscopy was used to follow the optical thickness and irregularity of cells exposed for up to 24 h to Tm in the presence or absence of pre-miR-301a (Fig. 9A). Healthy cells are irregular in shape and thin, whereas dying cells are round and thick [28,29,30,31,32,33]. As shown in Fig. 9B and C, a slight trending of dying cells was observed starting after 12 h exposure to Tm which significantly increased by 24 h and these changes correlated well with the percentage of healthy cells that is drastically declining after 24 h of Tm treatment. In contrast, Tm treated cells in the presence of pre-miR-301a remained healthy up to 24 h with no significant difference in the percentage of dying and healthy cells compared to the no stress controls (Fig. 9C).
To confirm that the interaction of hsa-miR-301a-3p with the binding site in GADD45A 3âUTR contributes to cell fate decisions, we used the same approach to follow the 16HBE14o- viability during ER stress in the presence of the specific target protector. As shown in Fig. 10, these experiments confirmed that specific protection of GADD45A mRNA from hsa-miR-301a-3p mediated degradation with the target protector significantly accelerated the cell death in Tm treated 16HBE14o-. Although the transfection with the Tp control was increasing cell death in both control cells and Tm treated in the ER stress exposed cells, the percentage of healthy cells was significantly lower in the presence of Tp starting from 6 h (Fig. 10B) that was well reflected in the percentage of dying cells (Fig. 10C).
Taken together, our data indicates that during UPR, IRE1 degrades pre-miR-301a and thus enhances the expression of GADD45A mRNA and protein which promotes the ER stressed induced cell apoptosis (Fig. 10D). This illustrates another function of IRE1 during the UPR.
Discussion
The UPR functions to restore ER homeostasis or in the presence of unmitigated stress conditions to promote cell death. Using immortalized human bronchial epithelial cells and pharmaceutical stressors, we focused on the role of IRE1, the most evolutionarily conserved UPR signaling pathway to understand IRE1âs potential role in the demarcation between the pro-survival programs and apoptosis. IRE1 is known to utilize its endoribonuclease activity for 2 processes: (1) to splice XBP1 mRNA to generate a functional transcription factor to induce a pro-survival pathway and (2) to cleave and inactivate mRNAs and miRNAs to reduce the ER load [9, 14, 39, 49,50,51]. IRE1 also induces the stress pathways driven by JUN N-terminal kinase (JNK) and by nuclear factor-ÎșB (NF-ÎșB) (reviewed in [4]). The goal here was to identify potential miRNAs that would influence either survival or apoptosis.
Previous reports including our own have reported that the global miRNA levels during ER stress are reduced during the UPR adaptive response [19, 40]. Given that the that IRE1-mediated RNA degradation involves miRNA [39, 49], it was plausible that this enzyme might be more directly responsible for UPR-related miRNA reduction in addition to the obvious loss of miRNAs in the RISCs. When we followed genome wide expression changes in ER stressed in 16HBE14o- [19], only 7 of the miRNAs were significantly reduced by at least 2 fold independent of the ER stress model in all the times tested (2-, 4-, 6- and 9-hours). 16HBE14o- cells are a commonly used model for studying human airway epithelia functions under both physiological and pathological conditions [18, 52]. This approach, however, does not consider the possibility that IRE1âs negative effects on miRNAs levels could be masked by the induction of these ncRNAs by the PERK and ATF6 UPR branches.
Previous studies had indicated that an important role of IRE1 in regulating miRNA levels during UPR includes both maturation and degradation of these noncoding RNAs [39, 49,50,51]. In mouse models, IRE1 has been shown to be responsible for a Dicer-independent maturation of pre-miR-2317 [50], as well as responsible for degradation of group of miRNAs (miRs-17, -34a, -96, -125b) that normally repress translation of Caspase-2 mRNA [39]. In mouse fibroblasts, inhibition of IRE1 resulted in accumulation of miR-150 [51]. The functional involvement of IRE1 in UPR-related regulation of human miRNAs, however, has not been reported. Our results directly support IRE1âs ability to cleave pre-miRNAs such as was shown for pre-miR-17 [16].
We used two pharmacological stress conditions to activate the UPR and pretreated cells with or without an IRE1 inhibitor (4”8C) that inhibits IRE1âs endonuclease activity [37] to isolate the effect of IRE1 in decreasing miRNAs levels. From Next-Generation sequencing analyses, we identified 12 miRNAs that were downregulated during tunicamycin treatment. During ER stress conditions, we treated the cells with or without the IRE1 endonuclease inhibitor and found that 3 of these miRNAs, miR-301a-3p, miR-106b-5p, and miR-17-5p were elevated after IRE1 endonuclease inhibition, suggesting the IRE1 was degrading these miRNAs. As a further test that the inhibitor was working under these conditions, we demonstrated that spliced XBP1 mRNA levels were also dramatically reduced by this treatment. Furthermore, one of these three microRNAs, mouse pre-miR-17, has been previously shown to be directly cleaved by recombinant human IRE1 in T-REx-293 cells at 3 cleavage sites including in a mature mouse miR-17-3p site [39].
We used pre-miRNA for two reasons. First, previous studies had indicated that IRE1 could promote maturation or degradation of them as mentioned above. Two, we wanted to limit the number of mature miRNAs in our transfections in order to limit the non-specific changes in gene expression that can sometimes occur with supraphysiological levels of mature miRNAs [53]. In this case, the use of pre-RNAs increased the miRNA levels 400-fold compared to the use of miRNA mimics which can elevate the cellular levels of miRNAs to hundreds of thousands of times [24, 54, 55].
To confirm these effects in airway epithelial cells, we tested IRE1âs role in processing or degrading pri-miR-17 or pre-miR-17 and whether these potential processes occurred in the nucleus or cytosol after 6 h of tunicamycin or thapsigargin treatment with or without the IRE1 inhibitor 4”8C. The results indicated the pre-miR-17, but not pri-miR-17, was decreased during both stressor treatments and this decrease was reversed in the presence of 4”8C. Furthermore, the results indicated that this occurred in both the nucleus and the cytosol. This supports previous studies by Scott Oakes and colleagues that in cell-free systems that recombinant IRE1α endonucleolytically cleaved mouse pre-miR-17 precursor at sites distinct from DICER but did not affect the pri-miR-17 levels [39]. Our results in human airway epithelial cells confirm their cell-free analyses.
Using the same analysis, we then tested the other two immature miRNAs, pre-miR-301a and pre-miR-106b. For pre-miR-301a, interestingly, most of the pre-miR is in the nucleus and very little in the cytosol. Furthermore, in both the nucleus and the cytosol, IRE1 inhibition in the presence of both stressors significantly elevates their expression, suggesting that IRE1 endonuclease activity is decreasing their levels during stress responses just like pre-mir-17. For pre-miR-106b, the same effect was seen with both stressors and the IRE1 inhibitor in the nucleus, but interestingly, it was only seen during tunicamycin treatment in the cytosol, but not seen with thapsigargin treatment and inhibitor. However, our follow up analyses of miRNA and pre-miRNA stability clearly indicate that in the ER stress-exposed cells, IRE1 is processing cytosolic pre-miRNAs, whereas the changes in the nuclear fraction of pre-miRNA are a consequence of the IRE1-containnig ER membrane continuity with the nuclear envelope (Supplemental Fig. 2C).
The potential targets of miR-301a-3p included an exact match for the 3â UTR of GADD45A, a known proapoptotic gene. Furthermore, this miRNA targeting sequence is well-conserved in a number of species. We monitored the activity of GADD45A mRNA levels during both stress conditions and found that IRE1 inhibition significantly decreased the GADD45A mRNA levels indicating that the endonuclease activity of IRE1 was either influencing GADD45A mRNA directly or indirectly through spliced XBP1 or a miRNA that affected GADD45A mRNA levels such as miR-301a-3p. To test IRE1âs effect was occurring through XBP1s, we used an inducible XBP1s cell line, and first tested if induction of XBP1s had any influence on GADD45A mRNA and found that GADD45A levels did not change. We cannot exclude that another factor such as p53 could contribute to UPR cell death-related induction of GADD45A expression. Our previous analysis focusing on open chromatin in HeLa cells identified p53 binding motifs in a region 2 kB upstream of the transcription start site of GADD45A [15]. Thus, despite the fact that TP53 expression was not increased in our ER stress models [15, 19, 20], we cannot exclude that phosphorylated p53 could also contribute to the increased GADD45A during apoptotic phase of UPR, as also suggested by the results of the gene expression network analysis [56] (Supplemental Fig. 4). This possibility suggests the potential crosstalk between the UPR/IRE1 and DNA damage response [57]. Indeed, many UPR apoptotic factors, including CHOP (aka GADD153), GADD34, as well as GADD45A and GADD45B are important for a proper response to genotoxic stress [16, 17, 57,58,59,60]. Notably, however both DNA damage-related activation of PERK and/or IRE1 pathways were often reported to have proadaptive functions [61,62,63]. Thus, depending on the cellular insult, same UPR/DNA damage-related factors may have different roles in cell fate decisions and therefore further studies are required to better understand the specific mechanisms underlying their multi-stress specific functions.
To test the role of miR-301a-3p, we utilized a target protector for the putative miR-301a-3p binding site in the 3âUTR of GADD45A. Using 16HBE14o- cells that were induced with either tunicamycin or thapsigargin, we found that both methods increased GADD45A levels, and that pre-miR-301 transfection significantly decreased GADD45A levels in both stressors, and that the addition of a target protector to the miR-301a-3p binding site in GADD45A 3âUTR was able to reverse this effect and increase GADD45A levels. Further support came from the use of an antagomir (anti-miR-301a-3p) that enhanced the GADD45A mRNA levels as well. Together, this indicated that IRE1âs inhibition of a pre-miR-301a during stress conditions facilitated GADD45A mRNA level increases that could lead to a pro-apoptotic response. It also indicated that the IRE1 activity could influence both pro-survival (splicing XBP1) and pro-apoptotic signaling pathways by degrading pre-miR-301a to elevate GADD45A levels. To confirm this hypothesis, we tested if during ER stress that inhibition of IRE1 endonuclease activity would [1] decrease GADD45A mRNA levels and increase pre-miR-301 levels and if this would [2] decrease GADD45α protein and both of these were confirmed.
IRE1âs effect suggested that its endonuclease activity prevented the maturation of a miRNA that targets a pro-apoptotic gene, GADD45A, and therefore enhanced GADD45A expression during stress conditions. The better-known effect of IRE1 is its endonuclease activity on unspliced XBP1 mRNA to generate spliced XBP1s mRNA that leads to a functional transcription factor that promotes cell survival. To test this model and the role of miR-301a-3p in this process, we performed real time and label free holographic microscopy-based monitoring of cell death and viability using HoloMonitorÂź time-lapse cytometer [28,29,30,31,32,33]. We followed the optical thickness and irregularity of cells exposed for up to 24 h to Tm in the presence or absence of pre-miR-301a. This analysis indicated that under control conditions greater than ~â10% of the cells are thick and round (dying), and after 12 and 24 h of tunicamycin treatment this increased to ~â25 and ~â65%, respectively, were dying. After transfection with pre-miR-301a, however, these numbers decreased to ~â10% and ~â25%, respectively, indicating that this miRNA dramatically reversed the level of apoptosis. This supports the idea that the IRE1 pathway has components that both support survival as well as apoptosis, and the balance of these responses and the level of GADD45A dictates the final result. A schematic model of this overview is shown in Fig. 10D.
To confirm that the interaction of hsa-miR-301a-3p with the binding site in GADD45A 3âUTR, we used the same approach to follow the 16HBE14o- viability during ER stress in the presence of the specific target protector. These experiments confirmed that specific protection of GADD45A from hsa-miR-301a-3p-mediated degradation with the target protector significantly accelerated the cell death in Tm treated 16HBE14o-. Although the transfection with the control Tp did increase cell death levels in both control cells and Tm treated, in the ER stress exposed cells, the percentage of healthy cells was significantly lower in the presence of GADD45A 3â UTR Tp starting at 6 h. This was also well reflected in the percentage of dying cells.
Conclusions
Taken together, the data indicate that during UPR, IRE1 degrades pre-miR-301a and thus enhances the expression of GADD45A and this switches the ER stressed cells to apoptosis. This analysis indicated that IRE1âs endonuclease activity is a two-edged sword that can both promote survival through increased spliced XBP1 expression or cell death through degradation of a miRNA that inhibits GADD45A expression levels during the UPR.
Availability of data and materials
All data generated or analyzed during this study are included in this published article (and its supplementary information files). Deep sequencing data were deposited in Gene Expression Omnibus (GEO) at accession numbers: GSE117629 and GSE129813.
Abbreviations
- 4”8C:
-
IRE1 Inhibitor III
- 16HBE14o:
-
Immortalized human bronchial epithelial cells
- ATF6:
-
Activating transcription factor 6
- ALLN:
-
Inhibitor of calpain 1
- BIP:
-
Binding immunoglobulin protein
- BSA:
-
Bovine serum albumin
- id:
-
Identification
- CYTB:
-
Cytochrome B
- DMSO:
-
Dimethyl sulfoxide
- ER:
-
Endoplasmic reticulum
- EREG:
-
Epiregulin
- FBS:
-
Fetal bovine serum
- GADD45A:
-
Growth arrest and DNA-damage-inducible alpha
- GAPDH:
-
Glyceraldehyde-3-phosphate dehydrogenase
- HeLa:
-
Human cervix adenocarcinoma cells
- IRE-1:
-
Inositol-requiring enzyme 1
- miRNA:
-
microrna
- NGS:
-
Next generation sequencing
- PERK:
-
PKRâlike endoplasmic reticulum kinase
- PBS:
-
Phosphate buffer saline
- qRT-PCR:
-
Quantitative real-time PCR
- RIDD:
-
IRE-1 dependent decay
- RNU6:
-
U6 small nuclear
- RNU44:
-
U44 small nuclear
- RPLP0:
-
Ribosomal protein lateral stalk subunit p0
- SD:
-
Standard deviation
- SDS:
-
Sodium dodecyl sulphate
- SDS-PAGE:
-
Sodium dodecyl sulphateâpolyacrylamide gel electrophoresis
- Tm:
-
Tunicamycin
- Tg:
-
Thapsigargin
- UPR:
-
Unfolded protein response
- UTR:
-
Untranslated region
- XBP-1s:
-
Spliced X-box binding protein 1
References
Schroder M, Kaufman RJ. ER stress and the unfolded protein response. Mutat Res. 2005;569(1â2):29â63.
Urra H, Dufey E, Lisbona F, Rojas-Rivera D, Hetz C. When ER stress reaches a dead end. Bba-Mol Cell Res. 2013;1833(12):3507â17.
Berridge MJ. The endoplasmic reticulum: a multifunctional signaling organelle. Cell Calcium. 2002;32(5â6):235â49.
Hetz C. The unfolded protein response: controlling cell fate decisions under ER stress and beyond. Nat Rev Mol Cell Bio. 2012;13(2):89â102.
Hetz C, Chevet E, Oakes SA. Proteostasis control by the unfolded protein response. Nat Cell Biol. 2015;17(7):829â38.
Ron D, Walter P. Signal integration in the endoplasmic reticulum unfolded protein response. Nat Rev Mol Cell Bio. 2007;8(7):519â29.
Lu M, Lawrence DA, Marsters S, Acosta-Alvear D, Kimmig P, Mendez AS, et al. Opposing unfolded-protein-response signals converge on death receptor 5 to control apoptosis. Science. 2014;345(6192):98â101.
Chang TK, Lawrence DA, Lu M, Tan J, Harnoss JM, Marsters SA, et al. Coordination between two branches of the unfolded protein response determines apoptotic cell fate. Mol Cell. 2018;71(4):629â.
Maurel M, Chevet E, Tavernier J, Gerlo S. Getting RIDD of RNA: IRE1 in cell fate regulation. Trends Biochem Sci. 2014;39(5):245â54.
Chevet E, Cameron PH, Pelletier MF, Thomas DY, Bergeron JJM. The endoplasmic reticulum: integration of protein folding, quality control, signaling and degradation. Curr Opin Struc Biol. 2001;11(1):120â4.
Almanza A, Carlesso A, Chintha C, Creedican S, Doultsinos D, Leuzzi B, et al. Endoplasmic reticulum stress signalling - from basic mechanisms to clinical applications. FEBS J. 2019;286(2):241â78.
Nikawa JI, Yamashita S. Ire1 encodes a putative protein-kinase containing a membrane-spanning domain and is required for Inositol Phototrophy in Saccharomyces-Cerevisiae. Mol Microbiol. 1992;6(11):1441â6.
Shore GC, Papa FR, Oakes SA. Signaling cell death from the endoplasmic reticulum stress response. Curr Opin Cell Biol. 2011;23(2):143â9.
Hollien J, Weissman JS. Decay of endoplasmic reticulum-localized mRNAs during the unfolded protein response. Science. 2006;313(5783):104â7.
Bartoszewski R, Gebert M, Janaszak-Jasiecka A, Cabaj A, Kroliczewski J, Bartoszewska S, et al. Genome-wide mRNA profiling identifies RCAN1 and GADD45A as regulators of the transitional switch from survival to apoptosis during ER stress. FEBS J. 2020;287(14):2923â47.
Sheikh MS, Hollander MC, Fornace AJ. Role of Gadd45 in apoptosis. Biochem Pharmacol. 2000;59(1):43â5.
Nelson JO, Moore KA, Chapin A, Hollien J, Metzstein MM. Degradation of Gadd45 mRNA by nonsense-mediated decay is essential for viability. Elife. 2016;5:e12876. https://doi.org/10.7554/eLife.12876.
Forbes B, Shah A, Martin GP, Lansley AB. The human bronchial epithelial cell line 16HBE14o- as a model system of the airways for studying drug transport. Int J Pharm. 2003;257(1â2):161â7.
Gebert M, Bartoszewska S, Janaszak-Jasiecka A, Moszynska A, Cabaj A, Kroliczewski J, et al. PIWI proteins contribute to apoptosis during the UPR in human airway epithelial cells. Sci Rep. 2018;8(1):16431.
Gebert M, Sobolewska A, Bartoszewska S, Cabaj A, Crossman DK, Kroliczewski J, et al. Genome-wide mRNA profiling identifies x-box-binding protein 1 (XBP1) as an IRE1 and PUMA repressor. Cell Mol Life Sci. 2021;78(21â22):7061â80.
Bartoszewska S, Cabaj A, Dabrowski M, Collawn JF, Bartoszewski R. miR-34c-5p modulates x-box-binding protein 1 (XBP1) expression during the adaptive phase of the unfolded protein response. FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology. 2019;33(10):11541â54.
Bartoszewski R, Rab A, Fu L, Bartoszewska S, Collawn J, Bebok Z. CFTR expression regulation by the unfolded protein response. Methods Enzymol. 2011;491:3â24.
Bartoszewska S, Kroliczewski J, Crossman DK, Pogorzelska A, Baginski M, Collawn JF, et al. Triazoloacridone C-1305 impairs XBP1 splicing by acting as a potential IRE1alpha endoribonuclease inhibitor. Cell Mol Biol Lett. 2021;26(1):11.
Bartoszewska S, Kamysz W, Jakiela B, Sanak M, Kroliczewski J, Bebok Z, et al. miR-200b downregulates CFTR during hypoxia in human lung epithelial cells. Cell Mol Biol Lett. 2017;22:23.
Livak KJ, Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 2001;25(4):402â8.
JaĆkiewicz M, MoszyĆska A, KrĂłliczewski J, et al. The transition from HIF-1 to HIF-2 during prolonged hypoxia results from reactivation of PHDs and HIF1A mRNA instability. Cell Mol Biol Lett. 2022;27:109.
Bartoszewski R, Moszynska A, Serocki M, Cabaj A, Polten A, Ochocka R, et al. Primary endothelial cell-specific regulation of hypoxia-inducible factor (HIF)-1 and HIF-2 and their target gene expression profiles during hypoxia. Faseb J. 2019;33(7):7929â41.
Kemmler M, Fratz M, Giel D, Saum N, Brandenburg A, Hoffmann C. Noninvasive time-dependent cytometry monitoring by digital holography. J Biomed Opt. 2007;12(6):064002.
Khmaladze A, Matz RL, Epstein T, Jasensky J, Holl MMB, Chen Z. Cell volume changes during apoptosis monitored in real time using digital holographic microscopy. J Struct Biol. 2012;178(3):270â8.
Kuhn J, Shaffer E, Mena J, Breton B, Parent J, Rappaz B, et al. Label-free cytotoxicity screening assay by Digital Holographic Microscopy. Assay Drug Dev Techn. 2013;11(2):101â7.
Molder A, Sebesta M, Gustafsson M, Gisselson L, Wingren AG, Alm K. Non-invasive, label-free cell counting and quantitative analysis of adherent cells using digital holography. J Microsc-Oxford. 2008;232(2):240â7.
Pavillon N, KĂŒhn J, Moratal C, Jourdain P, Depeursinge C, Magistretti PJ, Marquet P. Early cell death detection with digital holographic microscopy. PLoS One. 2012;7(1):e30912. https://doi.org/10.1371/journal.pone.0030912.
Ziegler U, Groscurth P. Morphological features of cell death. News Physiol Sci. 2004;19:124â8.
Trapnell C, Pachter L, Salzberg SL. TopHat: discovering splice junctions with RNA-Seq. Bioinformatics. 2009;25(9):1105â11.
Trapnell C, Williams BA, Pertea G, Mortazavi A, Kwan G, van Baren MJ, et al. Transcript assembly and quantification by RNA-Seq reveals unannotated transcripts and isoform switching during cell differentiation. Nat Biotechnol. 2010;28(5):511âU174.
Vandesompele J, De Preter K, Pattyn F, et al. Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes. Genome Biol. 2002;3:research0034.1. https://doi.org/10.1186/gb-2002-3-7-research0034.
Cross BC, Bond PJ, Sadowski PG, Jha BK, Zak J, Goodman JM, et al. The molecular basis for selective inhibition of unconventional mRNA splicing by an IRE1-binding small molecule. Proc Natl Acad Sci U S A. 2012;109(15):E869â78.
Oikawa D, Tokuda M, Hosoda A, Iwawaki T. Identification of a consensus element recognized and cleaved by IRE1 alpha. Nucleic Acids Res. 2010;38(18):6265â73.
Upton JP, Wang L, Han D, Wang ES, Huskey NE, Lim L, et al. IRE1α cleaves select microRNAs during ER stress to derepress translation of proapoptotic Caspase-2. Science. 2012;338(6108):818â22.
Mesitov MV, Soldatov RA, Zaichenko DM, Malakho SG, Klementyeva TS, Sokolovskaya AA, et al. Differential processing of small RNAs during endoplasmic reticulum stress. Sci Rep. 2017;7:46080.
Ha M, Kim VN. Regulation of microRNA biogenesis. Nat Rev Mol Cell Biol. 2014;15(8):509â24.
Watson ML. The nuclear envelope; its structure and relation to cytoplasmic membranes. J Biophys Biochem Cytol. 1955;1(3):257â70.
Prachar J. Intimate contacts of mitochondria with nuclear envelope as a potential energy gateway for nucleo-cytoplasmic mRNA transport. Gen Physiol Biophys. 2003;22(4):525â34.
Lee Y, Jeon K, Lee JT, Kim S, Kim VN. MicroRNA maturation: stepwise processing and subcellular localization. EMBO J. 2002;21(17):4663â70.
Kingston ER, Bartel DP. Global analyses of the dynamics of mammalian microRNA metabolism. Genome Res. 2019;29(11):1777â90.
Staton AA, Giraldez AJ. Use of target protector morpholinos to analyze the physiological roles of specific miRNA-mRNA pairs in vivo. Nat Protoc. 2011;6(12):2035â49.
Abcouwer SF, Schwarz C, Meguid RA. Glutamine deprivation induces the expression of GADD45 and GADD153 primarily by mRNA stabilization. J Biol Chem. 1999;274(40):28645â51.
MoszyĆska A, Collawn JF, Bartoszewski R. IRE1 Endoribonuclease Activity Modulates Hypoxic HIF-1α Signaling in Human Endothelial Cells. Biomolecules. 2020;10(6):895. https://doi.org/10.3390/biom10060895.
Avril T, Chevet E. IRE1-mediated miRNA maturation in macrophage phosphoinositide signaling. EMBO Rep. 2020;21(12):e51929.
Hamid SM, Citir M, Terzi EM, Cimen I, Yildirim Z, Dogan AE, et al. Inositol-requiring enzyme-1 regulates phosphoinositide signaling lipids and macrophage growth. EMBO Rep. 2020;21(12):e51462.
Heindryckx F, Binet F, Ponticos M, Rombouts K, Lau J, Kreuger J, et al. Endoplasmic reticulum stress enhances fibrosis through IRE1alpha-mediated degradation of miR-150 and XBP-1 splicing. EMBO Mol Med. 2016;8(7):729â44.
Bebok Z, Collawn JF, Wakefield J, Parker W, Li Y, Varga K, et al. Failure of cAMP agonists to activate rescued deltaF508 CFTR in CFBE41o- airway epithelial monolayers. J Physiol. 2005;569(Pt 2):601â15.
Jin HY, Gonzalez-Martin A, Miletic AV, Lai M, Knight S, Sabouri-Ghomi M, Head SR, Macauley MS, Rickert RC, Xiao C. Transfection of microRNA Mimics Should Be Used with Caution. Front Genet. 2015;6:340. https://doi.org/10.3389/fgene.2015.00340.
Calabrese JM, Seila AC, Yeo GW, Sharp PA. RNA sequence analysis defines Dicerâs role in mouse embryonic stem cells (vol 104, pg 18097, 2007). P Natl Acad Sci USA. 2007;104(52):21021.
Bartoszewski R, Serocki M, Janaszak-Jasiecka A, Bartoszewska S, Kochan-Jamrozy K, Piotrowski A, et al. miR-200b downregulates Kruppel like factor 2 (KLF2) during acute hypoxia in human endothelial cells. Eur J Cell Biol. 2017;96(8):758â66.
Zhou G, Soufan O, Ewald J, Hancock REW, Basu N, Xia J. NetworkAnalyst 3.0: a visual analytics platform for comprehensive gene expression profiling and meta-analysis. Nucleic Acids Res. 2019;47(W1):W234âW41.
Bolland H, Ma TS, Ramlee S, Ramadan K, Hammond EM. Links between the unfolded protein response and the DNA damage response in hypoxia: a systematic review. Biochem Soc Trans. 2021;49(3):1251â63.
Bartoszewska S, SĆawski J, Collawn JF, Bartoszewski R. Dual RNase activity of IRE1 as a target for anticancer therapies. J Cell Commun Signal. 2023. https://doi.org/10.1007/s12079-023-00784-5.
Das B, Samal S, Hamdi H, Pal A, Biswas A, Behera J, et al. Role of endoplasmic reticulum stress-related unfolded protein response and its implications in dengue virus infection for biomarker development. Life Sci. 2023;329:121982.
Eid JI, Al-Tuwaijri MM, Mohanty S, Das B. Chaga mushroom (Inonotus obliquus) polysaccharides exhibit genoprotective effects in UVB-exposed embryonic zebrafish (Danio rerio) through coordinated expression of DNA repair genes. Heliyon. 2021;7(2):e06003.
Wingert S, Thalheimer FB, Haetscher N, Rehage M, Schroeder T, Rieger MA. DNA-damage response gene GADD45A induces differentiation in hematopoietic stem cells without inhibiting cell cycle or survival. Stem Cells. 2016;34(3):699â710.
Xie M, Xie R, Huang P, Yap DYH, Wu P. GADD45A and GADD45B as novel biomarkers Associated with chromatin regulators in Renal Ischemia-Reperfusion Injury. Int J Mol Sci. 2023;24(14):11304.
Friedman RC, Farh KK, Burge CB, Bartel DP. Most mammalian mRNAs are conserved targets of microRNAs. Genome Res. 2009;19(1):92â105. https://doi.org/10.1101/gr.082701.108.
Friedman RC, Farh KKH, Burge CB, Bartel DP. Most mammalian mRNAs are conserved targets of microRNAs. Genome Res. 2009;19(1):92â105.
Acknowledgements
We would like to thank Dr. David Crossman (University of Alabama at Birmingham) for the help with GEO submission.
Funding
This research was funded by National Science Center âOpusâ Program under contract UMO-2020/37/B/NZ3/00861 (R.B.). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
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Magda Gebert Sylwia Bartoszewska and Lukasz Opalinski performed the experiments. All authors contributed to the study conception and design, material preparation and data analysis. The first draft of the manuscript was written by James F. Collawn, Rafal Bartoszewski and Magda Gebert. All authors read and approved the final manuscript.
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Gebert, M., Bartoszewska, S., Opalinski, L. et al. IRE1-mediated degradation of pre-miR-301a promotes apoptosis through upregulation of GADD45A. Cell Commun Signal 21, 322 (2023). https://doi.org/10.1186/s12964-023-01349-0
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DOI: https://doi.org/10.1186/s12964-023-01349-0