Fig. 2

IRE1 inhibition during ER stress reduces XBP1s expression (A and B) and restores hsa-miR-17-5p (C), hsa-miR-301a-3p (D) and hsa-106b-5p levels (E). hsa-miRs, and XBP1s mRNA levels were quantified with qRT-PCR and normalized to RPLP0 mRNA levels and expressed as a fold change compared to the no-stress control sample. Data represents the mean ± SD of three independent experiments (3 replicates each). * P < 0.05 was considered significant. The changes in XBP1s protein levels were evaluated by western blots normalized to total protein levels. Changes in hsa-miR-301a-3p, hsa-miR-106b-5p, hsa-miR-17-5p were quantified by qRT-PCR and the results from three independent experiments (n = 9) were plotted normalized to RNU44 levels and expressed as a fold change over the no-stress controls. Error bars represent standard deviations. Significant changes (P value P < 0.05) are marked with an asterisk. ER stressors used: Tm (2.5 µg/ml), Tg (50 nM)). The 4µ8C was used at a 20 µM concentration. F The Tm (2.5 µg/ml) treatment does not affect the miRNA biogenesis component expression. AGO2, DICER1 and DROSHA expression levels were obtained from 3 independent NGS-based analysis of transcriptome changes during ER stress [15, 19]. Error bars represent standard deviations