Fig. 4
From: IRE1-mediated degradation of pre-miR-301a promotes apoptosis through upregulation of GADD45A
The pre-miR precursor of hsa-miR-301a-3p and hsa-miR-106b-5p are concentrated in nuclear fraction and sensitive to IRE1 activity. The impact of 4µ8C on pre-miR-301a (A and B) as well as pre-miR-106b in both nuclear and cytosolic fractions from ER stress exposed cells (C and D). RNA levels were quantified with qRT-PCR and normalized to RNU44 and expressed as fold change over the no-stress control samples. The results from three independent experiments (n = 9) are plotted. Error bars represent standard deviations. Significant changes (P value P < 0.05) are marked with an asterisk. ER stressors used: Tm (2.5 µg/ml), Tg (50 nM)). The 4µ8C was used at a 20 µM concentration