Fig. 3
From: IRE1-mediated degradation of pre-miR-301a promotes apoptosis through upregulation of GADD45A
The pre-miR precursor of hsa-miR-17-5p is concentrated in the nuclear fraction and sensitive to IRE1 activity. qRT-PCR based analyses of nuclear and cytosolic fraction purities based on quantification of pri-miR-17 (A) and CYTB (B) expression. RT-qPCR results from three independent experiments (n = 9) are plotted normalized to RPLP0 levels and expressed as a fold change over the no-stress controls. Error bars represent standard deviations. Significant changes (P value P < 0.05) are marked with an asterisk. ER stressors used: Tm (2.5 µg/ml), Tg (50 nM)). The impact of 4µ8C (IRE1 inhibitor) on pri-miR-17 (C) as well as pre-miR-17 RNA levels in both nuclear and cytosolic fractions from ER stress exposed cells (D and E) were quantified with qRT-PCR and normalized to RPLP0 (for pri-miR-17) or RNU44 and expressed as a fold change over no-stress control sample. The results from three independent experiments (n = 9) are plotted. Error bars represent standard deviations. Significant changes (P value P < 0.05) are marked with an asterisk. ER stressors used: Tm (2.5 µg/ml), Tg (50 nM)). The 4µ8C was used at a 20 µM concentration