Fig. 10

hsa-miR-301a-3p modulates GADD45A levels during ER stress and contributes to cell death decisions. The results of real-time monitoring of cell viability using holographic microscopy (HoloMonitor M4® time-lapse cytometer). 16HBE14o- cells were transfected with target protector (Tp) or Tp control and treated with Tm (2.5 µg/ml) up to 24 h. Images were collected every hour from 5 independent optical fields. The distribution of live (blue), dying cells (red), and dead cells (grey) were based on their optical thickness and irregularity is presented only at the 6- and 12-hour time points (A). The images from up to 5 independent optical fields were collected and analyzed according to manufacture instructions with HoloMonitor® App Suite software. One representative optical field is presented in the right panels. For all of the analyses, the same cells parameter quantification was applied as in Fig. 9. Experiments were performed in triplicates. Based on the cells irregularity and average optical thickness the percentages of healthy cells (B) and of dying cells (C) were calculated. Data represents the mean ± SD of three independent experiments. * P < 0.05 was considered significant. Schematic representation of role of IRE1-mediated degradation of pre-miR-301a on the cell fate decision during ER stress (D)