Fig. 6
From: IRE1-mediated degradation of pre-miR-301a promotes apoptosis through upregulation of GADD45A
Inhibition of IRE1 activity during ER stress decreases GADD45A mRNA levels. Schematic representation of hsa-miR-301a-3p binding site in the GADD45A 3’ UTR sequence as predicted by Target scan 6.0 (A). The conservation of predicted binding site in GADD45A sequence, as marked with red box (B) and conservation of hsa-miR-301a-3p seed sequence were analyzed with Target scan, as marked with red box (C) [64]. ER stress-induced changes in GADD45A, mRNA levels in 16HBE14o- cells (D) and the impact of 4µ8C on GADD45A expression (E) were analyzed with RT-qPCR and normalized to RPLP0 mRNA levels and expressed as a fold changeover no-stress control sample. The results from three independent experiments (n = 9) are plotted and expressed as a fold change over the no-stress controls. Error bars represent standard deviations. Significant changes (P value P < 0.05) are marked with an asterisk. ER stressors used: Tm (2.5 µg/ml), Tg (50 nM))