Fig. 1
From: IRE1-mediated degradation of pre-miR-301a promotes apoptosis through upregulation of GADD45A
The impact of the IRE1 inhibition on ER-stress exposed 16HBE14o- genome-wide miRNA profiles. A miRNA expression was significantly (P < 0.05) restored upon IRE1 inhibition with 4µ8C in tunicamycin (Tm, 2.5 µg/ml) treated cells and ALLN – Calpain I inhibitor (100 µM) treated cells) based on NGS profiling. The 4µ8C was used at 20 µM concentration. Bold font indicates the presence of an IRE1 consensus motif. B Impact of IRE1 inhibition on miRNAs reported previously as IRE1 targets. Bold font indicates the presence of an IRE1 consensus motif. C Schematic representation of an IRE1 motif in selected miRNAs precursors (pre-miRNA) stem loops. Mature miRNA-5p sequences are marked with blue, and mature miRNA-3p sequences in red. Arrows mark the IRE1 consensus motif sequence. ER stress-induced changes in (D) hsa-miR-301a-3p, E hsa-miR-106b-5p, F hsa-miR-17-5p, and (G) XBP1s RNA levels in 16HBE14o- cells. The results from three independent experiments (n = 9) are plotted normalized to RNU44 and RPLP0 mRNA levels and expressed as a fold change over the no-stress controls. Error bars represent standard deviations. Significant changes (P value P < 0.05) are marked with an asterisk. ER stressors used: Tm (2.5 µg/ml), Tg (50 nM))