Fig. 8
From: IRE1-mediated degradation of pre-miR-301a promotes apoptosis through upregulation of GADD45A
IRE1 and pre-miR-301a affect GADD45α levels during ER stress. 16HBE14o- cells treated with and without an IRE1 inhibitor (4”8C) and were treated with Tm (2.5 ”g/ml) for 6 h (A and B). The 16HBE14o- cells were first transfected with pre-miR-301a-3p or scramble control and two days later were treated with Tm (2.5 ”g/ml) for 6 h (C and D). The corresponding changes in GADD45α protein levels were monitored with Western blot that was normalized to total protein levels (A and C) and related to the respective control. Data represent the meanâ±âSD of 3 independent experiments. *Pâ<â0.05 was considered significant