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Crk and CrkL adaptor proteins: networks for physiological and pathological signaling
Cell Communication and Signaling volume 7, Article number: 13 (2009)
The Crk adaptor proteins (Crk and CrkL) constitute an integral part of a network of essential signal transduction pathways in humans and other organisms that act as major convergence points in tyrosine kinase signaling. Crk proteins integrate signals from a wide variety of sources, including growth factors, extracellular matrix molecules, bacterial pathogens, and apoptotic cells. Mounting evidence indicates that dysregulation of Crk proteins is associated with human diseases, including cancer and susceptibility to pathogen infections. Recent structural work has identified new and unusual insights into the regulation of Crk proteins, providing a rationale for how Crk can sense diverse signals and produce a myriad of biological responses.
Crk has a story book history, first emerging in the late 1980s as a novel retroviral gene product, v-Crk or Gag-Crk, and later serving as a major impetus for unraveling how modular protein domains assemble into organized protein-protein networks during signal transduction. The study of its mechanism of action has been full of unexpected and interesting findings, beginning first with a paradox as to how an oncogene product without intrinsic tyrosine kinase activity strongly and selectively increases cellular tyrosine phosphorylation levels. v-Crk and its cellular homologs, Crk II, Crk I, and the paralog CrkL, comprise the prototype of a novel class of regulatory proteins, called adaptors, composed of modular Src Homology 2 (SH2) and Src Homology 3 (SH3) domains separated by flexible linker sequences that act as building blocks to assemble multiprotein complexes. SH2 domains are structurally conserved protein domains of ~100 amino acids contained within the Src oncogene and other signaling proteins that bind tyrosine phosphorylated proteins in the context of short peptide sequences and localize SH2 domains to tyrosine phosphorylated proteins. SH3 domains are structurally conserved domains of ~60 amino acids that bind a consensus sequence of X1-P2-p3-x4-P5 where 1 and 4 are aliphatic amino acids, 2 and 5 are always proline, and together this sequence binds to the hydrophobic pocket of the SH3 domain. There are over 110 SH2 domains and 300 SH3 domains in the human genome, making this general signaling strategy widely utilized in metazoan cells to transmit intracellular signals. As the name adaptor implies, these molecules physically bridge tyrosine phosphorylated proteins to various intracellular signaling pathways (Figure 1A). An impressive body of work over the past two decades has demonstrated that the signal transduction functions of v-Crk, c-Crk, and CrkL are attributed to the formation of coordinately regulated protein complexes that bind to the SH2 and the more N-terminal SH3 domain (SH3N) (Figure 1B). The Crk SH2 domain binds short tyrosine phosphoryated proteins in the context of pTyr-Asp-x-Pro, and the SH3N domain binds to proteins with signature proline-rich sequences in the context of Pro-x-x-Pro-x-Lys/Arg (where x is any amino acid). To function as an adaptor protein, both the SH2 and SH3N domains need to be operational in time and space, acting as molecular adhesives to draw disparate information together to spatially and temporally regulate signal transduction pathways.
v-Crk, c-Crk, and CrkL family members
The Crk gene product was identified approximately 20 years ago in the form of a transforming gene (Gag-Crk) encoded in the genome of a defective avian sarcoma retrovirus called CT10 (c hicken t umor Virus 10) [1, 2]. A year later, Vogt and colleagues characterized an independent avian retrovirus (called ASV-1) isolated from a spontaneous tumor in an adult chicken with an oncogene product virtually identical to Gag-Crk . The most outstanding feature of CT10- or ASV-1 transformed chicken embryo fibroblasts (CEFs) results from a selective increase in tyrosine phosphorylation of cellular proteins of pp70 and pp130, initially suggesting CT10 virus encoded a tyrosine kinase oncogene [4, 5]. However, there were clear differences in the molecular characteristics of the CT10 virus compared to viruses that encoded tyrosine kinases, including the lack of induction of a classic "refractive morphology" in CEFs transformed by Rous sarcoma virus (RSV). Moreover, Northern blotting against a battery of 19 DNA fragments from previously characterized RNA tumor viruses, including many of the known tyrosine kinase oncogenes, did not hybridize with CT10 viral fragments, suggesting that the oncogene in CT10 was different from all previously identified tyrosine kinase oncogenes . Subsequently, a cDNA encoding the CT10 transforming gene was isolated and shown to encode a novel gene product containing viral gag sequences fused to sequences similar to those previously identified in the N-terminal regulatory region of the Src family kinases. Because v-Crk lacked intrinsic tyrosine kinase activity, Mayer and colleagues named the new gene with an acronym for its proposed function as a transactivator of a cellular tyrosine kinase, called Crk or C T10 r egulator of a tyrosine k inase.
Following the cloning of v-crk or gag-crk, Reichman, et al. reported the isolation of the cellular homolog of Crk by screening a chicken brain library using a portion of the v-crk probe devoid of the retrovirus-derived gag sequences . Molecular cloning of chicken cellular crk (c-crk) revealed a molecule with similar structural organization to v-Crk, but containing a ~50 amino acid proline-rich linker and an additional C-terminal SH3 domain (SH3C) (Figure 1B). Matsuda and colleagues characterized two species of Crk in mammalian cells, Crk II and Crk I, derived from alternative splicing from a single gene locus . Structurally, Crk I is more analogous to v-Crk and, biologically, significantly more transforming than Crk II when ectopically expressed in NIH 3T3 cells. The oncogenic activation of Crk by C-terminal truncation in the retroviral genome is reminiscent of the mode of oncogenic activation of tyrosine kinases, including the activation of Src in the RSV genome [8, 9]. Indeed, it is now clear that the differences in transforming activities of Crk II and Crk I rely, at least in part, on a specific post-translational modification of Crk that involves phosphorylation on Y222 in chicken (Y221 in mouse and human) . Both Crk II and CrkL are negatively regulated by autoinhibitory phosphorylation events that ultimately block the ability of Crk to function as an adaptor protein. This is analogous to the C-terminal tyrosine phoshorylation of Src family kinases by C-terminal Src Kinase (CSK) [11, 12], which allows Src to adopt an intramolecular pTyr-SH2 "closed" structure involving the Src SH2 and a C-terminal phosphorylation motif . Phosphorylation of Crk on Tyr 221 (or CrkL on Tyr 207) causes intramolecular binding of the linker region to the SH2 domain, sequestering the SH2 and SH3N and preventing them from binding target proteins [14, 15]. Subsequent studies have shown that the negative regulation of Crk II occurs directly via the activity of the proto-oncogene products Abl and Arg tyrosine kinases, the former originally characterized from the Abelson murine leukemia virus (A-MuLV) . Abl and Arg appear to be the predominant tyrosine kinases that negatively regulate Crk II (and CrkL) in cells, as CrkY221 phosphorylation is virtually undetectable in Abl/Arg (-/-) double knockout cells. Current theory holds that v-Crk and Crk I are more transforming than Crk II, mainly because their C-termini end prior to the regulatory Y221 site, although there are mutations in the v-crk gene that also may contribute to oncogenicity . Hence, v-Crk has no clear mechanism to downregulate signals, essentially being locked as a constitutively active adaptor in signal transduction.
Crk effector pathways: Molecular multi-tasking and signal plasticity
The Crk and CrkL proteins have central roles in an astonishingly vast number of biological processes, ranging from cell proliferation, cell adhesion and migration, phagocytic and endocytic pathways for apoptotic cells and parasitic organisms respectively, apoptosis, and regulation of gene expression . Until the introduction of siRNA technology and knockouts in genetically amenable systems, the majority of studies on Crk relied on identification of binding proteins to specific domains or by overexpression of wildtype or dominant negative Crk proteins in reconstituted systems. Because the SH2 and SH3 domains are modular and retain their binding specificity and affinity when expressed as isolated fragments [4, 18], GST (glutathione S transferase)-pull-down assays, Far-Western blotting, and yeast two-hybrid approaches permitted the initial characterization of Crk binding partners and inference of the participating signal transduction pathways.
Over the past two decades, over 40 cellular proteins have been identified that bind to the SH2 and SH3N domains, and for this reason, it is clearly daunting to assign one specific cellular function to Crk . Indeed, a PubMed search combining "Crk" and "signaling" returns over 700 articles, and survey of this literature clearly indicates that the adaptor function of Crk is used repetitively and often as a signal transduction strategy in the context of both physiological and pathophysiological situations (Table 1; and references [2, 5, 19–73]). Despite such complexity in Crk transduction outcomes, some general rules apply regardless of the specific proteins involved. First, the Crk SH2 domain (the input pathway) binds phosphotyrosine-containing proteins in an inducible on-and-off switch mechanism that involves regulated tyrosine phosphorylation and dephosphorylation. Second, the interactions between the Crk SH3N domain (the output pathway) occur constitutively, and these complexes are generally regulated without post-translational modifications, although there are a few exceptions whereby tyrosine phoshorylation has been shown to impinge on SH3-proline-rich assemblages . Therefore, while assigning functions to Crk, it is important to keep in mind that input signals depend on the availability of extracellular hormones and growth factors, the prior history of the cell, including whether the cell has been stressed or re-stimulated, the state of proliferation or differentiation of the cell, and other temporal and spatial epigenetic factors that reflect the physiological and metabolic status of the cell. As the input signals reflect changes in the cellular environment, Crk signals are subject to dynamic and plastic regulation, allowing for rapid and dynamic fluxes through signal transduction pathways that permit pleiotropic changes in the signaling capabilities of cells. Despite a detailed understanding of the types of interactions that exists between Crk and its substrates, there is still a paucity of information as to how these signals are integrated in real time and space.
Another important aspect of Crk signaling that requires definition is to assess which signaling pathways depend exclusively on Crk or CrkL versus those signaling pathways whereby Crk and CrkL can compensate for each other. Despite considerable homology between Crk II and CrkL in their SH2 and SH3 domains, development of knockout mouse models clearly show these gene products have distinct, non-overlapping roles, during embryonic development as both Crk knockout and CrkL knockout mice die perinatally with different developmental defects. Moreover, in Crk II (-/-) fibroblasts, CrkL proteins were not overexpressed in a compensatory manner, and vice versa of CrkII expression in CrkL (-/-) cells, again suggesting little molecular crosstalk at the level of expression in embryonic cells . Interestingly, mice homozygous for a null mutation of CrkL show a phenotype characteristic of DiGeorge/velocardiofacial syndrome, in which neural crest derived cells fail to differentiate and migrate leading to defects in cranial and cardiac development [76–78]. Crk null mice (which lack both Crk II and Crk I) die perinatally due to defects in cardiac and skeletal development . Imaizuma and colleagues generated another mouse using an exon trap method that lacks Crk II but still expresses Crk I. Although Crk I expressing mice show no obvious developmental abnormalities, it is not known whether these mice develop age-dependent malignancies, since they die within a few days after birth by unknown mechanisms .
However, in contrast to the aforementioned observations showing independent roles for CrkL and Crk II during development, a number of recent studies suggest that Crk II and CrkL are co-expressed in somatic cells and can compensate to each in cellular signaling. An example of such redundancy comes from several independent studies dissecting the Reelin signaling pathway in cortical pyramidal cells of the hippocampus. Reelin, a secreted glycoprotein that binds to receptors on hippocampal neurons such as ApoER2, VLDLR, and α3β1 integrin, controls cell positioning during adult neurogenesis via Src-dependent tyrosine phosphorylation of the scaffold protein Dab1 . Upon receptor activation, Dab1 becomes tyrosine phosphorylated at multiple sites, and binds a variety of SH2 domain-containing proteins that include both Crk II and CrkL [82, 83]. Studies using shRNA or cre-lox deletion of Crk and CrkL showed that knockdown of both proteins are required for the defects in neuronal positioning, and both proteins appear to converge on the activation of Rap1 through C3G (see below) [84–86]. How universal Crk and CrkL compensation is in signal transmission still remains an active area of investigation.
Role of p130Cas and molecular scaffolds in signal integration and Crk function
Much of the early emphasis on Crk biology focused on the mechanisms of increased cellular tyrosine phosphorylation and models for v-Crk transformation. Although it is still reasonable to consider that v-Crk binds to and "hijacks" a cellular tyrosine kinase, no model in which a specific tyrosine kinase is activated has provided a unifying molecular mechanism for v-Crk and Crk I transformation. However, a number of studies indicate that expression of v-Crk in Src (-/-) or Fyn (-/-) fibroblasts significantly reduces Crk-inducible tyrosine phosphorylation , and such studies imply that v-Crk transformation, at least in part, requires initial activation of a Src family kinase member (SFK). Subsequently, Akagi and colleagues demonstrated that focal adhesion kinase (FAK) Y397 phosphorylation (mediated by SFKs), and the recruitment of phosphatidylinositol 3'-OH kinase (PI3K) to FAK was also required for v-Crk-dependent transformation of CEFs [88, 89]. This observation is consistent with recent studies employing Src/Fyn/Yes (SFY)-deficient MEFs, whereby Src was shown to be required for FAK tyrosine phosphorylation and Crk-mediated motility .
With respect to the substrates of tyrosine phosphorylation and Crk transformation, a great deal of attention has been placed on the p130Cas/HEF1/Efs proteins, a family of non-enzymatic docking proteins that contain multiple interacting domains and serve as important anchoring points for protein-protein interactions [46, 91]. p130Cas (C rk a ssociated s ubstrate), the most ubiquitously expressed member, was in fact discovered as a result of its elevated phosphotyrosine levels observed in CT10-tranformed cells [5, 19, 92]. The importance of p130Cas in Crk and Src transformation has been illustrated not only because p130Cas stably and persistently binds SFKs, but also from studies that tyrosine kinase and Ras oncogenes fail to transform p130Cas (-/-) fibroblasts [93, 94]. Recent studies also indicate that p130cas is necessary and sufficient to transform cells, as a transgenic MMTV-p130Cas mouse that overexpresses p130Cas in the mammary gland shows extensive mammary epithelial hyperplasia during development and pregnancy with an association of elevated Src activity . Moreover, a double transgenic line expressing MMTV-p130Cas and MMTV-HER2-neu developed multifocal mammary tumors at an accelerated level . Overexpression of p130Cas also renders breast cancer cells more resistant to cytotoxic chemotherapies, such as anti-estrogen agents or adriamycin, indicating that p130Cas activates survival and metastatic pathways when tyrosine phosphorylated [96, 97]. Indeed, these studies are consistent with the fact that highly metastatic breast cancer epithelial cells up-regulate β1 integrin and p130Cas, and show stress fiber formation that correlates with EMT and motility from the site of tumor origin . Although to our knowledge v-Crk transformation has not been reported in p130Cas (-/-) cells, expression of a p130Cas substrate trap (which essentially contains just the Crk substrate region) effectively abrogates v-Crk transformation . Since many tumor cells express high amounts of tyrosine phosphorylated p130Cas, these and other studies implicate p130cas as a central component for Crk transformation.
Mice with homozygous null mutations of p130Cas exhibit marked growth retardation with poorly developed heart and vasculature and die in utero from congestive heart failure, edema, and hemorrhaging due to breakdown in the regulation of vascular permeability . Isolated and immortalized p130Cas (-/-) embryonic fibroblasts display short disorganized actin filaments, show defects in actin bundling, and these cells have small and poorly organized focal adhesions. However, a functional role for p130Cas in non-motile cell types, including neurons, is likely equally important and recent studies in Drosophila showed that targeted loss of p130Cas in CNS neurons induced defects in neurite guidance and target fasciculation . In fibroblasts, v-Crk localizes primarily at focal adhesions, and for this reason, the association of Crk with events associated with p130Cas and actin cytoskeleton assemblages has gained considerable acceptance in the Crk transformation field [101, 102]. Specific targeting of Crk to focal adhesions by fusing a FAT sequence to Crk induces p130Cas and FAK phosphorylation and potentiates cell migration .
At the molecular level, p130Cas has multiple protein-protein interaction domains including an N-terminal SH3 domain that binds FAK and Pyk2, an interior "substrate domain" characterized by 15 YxxP motifs, a C-terminal Src binding domain, followed by a highly conserved C-terminal region that binds to the Nsp family of proteins (Nsp1, And-34, and Chat)  (Figure 2A). Based on the unusual arrangement of repetitive YxxP motifs, there has been continued interest and conjecture as to the reasons why this motif has been duplicated 15 times, since when phosphorylated, they comprise a consensus binding site for the SH2 domain of Crk. Interesting studies by Miller and colleagues showed that the p130Cas substrate region was processively phosphorylated when Src bound to the polyproline-region of p130Cas, initially suggesting that the order of addition of phosphates may organize a pattern to prioritize a signaling mechanism . However, their more recent studies suggests that p130Cas phosphorylation is stochastic, meaning that no single "lynchpin" permits phosphorylation at additional sites and that the phosphorylation does not appear to follow an obligatory sequence . This would suggest that the juxtaposed repetitiveness of the YxxP motifs in p130Cas would mainly function in signal amplification to maximize the Crk output pathways, but also to diversify signaling, as functional cooperation in the Crk assemblages would also increase the repertoire of output signals, for example during integrin-mediated spreading and migration on extracellular matrix (ECM) molecules  (Figure 2C). Assuming that several of the tyrosines in the YxxP motifs are concomitantly phosphorylated at any given time, this might indicate that separate molecular complexes of p130Cas/Crk/DOCK180, p130Cas/Crk/C3G, p130Cas/Crk/Abl, p130Cas/Crk/JNK, and p130Cas/Crk/PI3K could exist simultaneously, but this remains to be seen experimentally (Figure 2C). Clearly, the development of site-specific, phospho-specific antibodies aimed to quantify and map the timing and sequence of p130Cas phosphorylation events could be used to determine whether there are more subtle patterns in the motifs that are phosphorylated, or perhaps more importantly, whether the half lives of individual phosphorylation events vary during signal downregulation. Such multiplicity in p130Cas phosphorylation could achieve extraordinary diversification in signaling by acting as a molecular scaffold to transmit localized subcellular signals.
The biological role of p130Cas phosphorylation is also intriguing from the vantage point that a vast variety of extracellular matrix molecules, growth factors, hormones, and metabolic intermediates that induce tyrosine phosphorylation of p130Cas and subsequent coupling to Crk (Table 1). In addition, recent studies have demonstrated that p130Cas phosphorylation is sensitive to physical transduction by mechanical force  and this may provide a common element for convergence in signal transduction (Figure 2B). These most interesting studies showed that when cells are cultured on a stretchable substrate consisting of collagen-coated flexible silicone to uniformly and biaxially stretch cells by 10% or more, p130Cas was inducibly tyrosine phosphorylated within 1 minute of the stretch. Although these experiments were designed to mimic integrin-mediated events, it is important to note that tyrosine phosphorylation of p130Cas occurred in the absence of specific integrin ligation and outside to inside integrin signaling. Moreover, using a clever FRET-based assay to monitor stretching of the p130Cas substrate domain (SD) in vitro, these investigators showed extension-dependent phosphorylation of the SD by both Src and Abl tyrosine kinases, but not CSK or ZAP-70. These data suggest that mechanical stretching of the substrate region, presumably by actin-tethering proteins brought together by binding to FAK and/or Src, is sufficient to cause p130Cas phopshorylation and subsequent coupling to downstream pathways (Figure 2C). This data also suggests that physiological events that induce stretching, i.e. adhesion, motility and engulfment of large particles, as well as pathophysiological events such as hypertrophy, are probably sufficient to induce p130Cas tyrosine phosphorylation, independent of the particularities that initiate the signal.
Once extended, the central region of p130Cas is a substrate for activated tyrosine kinases, and p130Cas itself has binding sites for FAK  and Pyk2  (via the SH3 domain) and SFK members through the C-terminal Src binding region (SBR)  (Figure 2A). In vivo, both of these kinases have been proposed to contribute to p130Cas phosphorylation, possibly to permit different mechanisms and different input signals to converge on assembling Crk linkages . To gain a better perspective on the specific role of Src versus FAK in mediating p130Cas phosphorylation, and determine whether phosphorylation is sufficient to activate downstream signaling, Sharma and Mayer  developed an approach called functional interaction trapping (FIT). Using this strategy, two signaling molecules are brought together by engineering fusion proteins with artificial binding surfaces composed of two leucine zipper coiled-coiled domains of ZipA and ZipB. When FIT was employed to bring together full-length p130Cas or the substrate region of p130Cas and Src, p130Cas was heavily phosphorylated on tyrosine and phosphorylated 130cas was sufficient to activate Rac1 and localize to focal adhesions . In apparent contrast, when FAK was fused with ZipB and expressed with p130Cas, p130Cas was weakly phosphorylated. This result appears to resolve a long-standing question as to whether FAK acts principally as a scaffold, since the Tyr397 site in FAK can recruit Src family kinase through its SH2 domain. However, functional studies show that FAK binding is important for p130Cas tyrosine phosphorylation in vivo, as fibroblasts derived from mice expressing a truncated p130Cas that lacks exon 2, and hence the N-terminal SH3 domain that binds FAK, fail to induce p130Cas phosphorylation when fibroblasts were induced to spread on FN .
Analogous to the theme described above for p130Cas, other scaffolding molecules that are acted upon by extrinsic signals ultimately interact with Crk, thereby launching alternative versions of a common signaling paradigm. Numerous stimuli, ranging from growth factors and cytokines to ECM, apoptotic cells, microbial products, immune complexes, and endogenous metabolic products activate trans-membrane receptors that inducibly phosphorylate proteins that, in turn, engage the Crk SH2 domain (Table 1). These proteins include Dab1 , IRS-1 , Paxillin , Gab1 , and CasL/Efs , and analogous to p130Cas, these proteins contain tandem YxxP motifs. As pointed out by Feller, et al., examination of the databases shows that the occurrence of three or more YxxP motifs in 100 kDa intracellular proteins is rare , raising the question of how such tandem elements are designed for functional crosstalk between Crk adaptor, or other adaptor protein pathways. As alluded to above, utilization of different scaffolds could allow for localized signaling, by virtue of being targeted by specific kinases, or could control the selectivity of Crk signaling. A good illustration of this comes from a study by Lamorte and colleagues, showing that a switch from a p130Cas/Crk to a Gab1/Crk complex in Met oncogene transformed cells correlates with a change from a motile phenotype to a proliferation phenotype . In a few cases, Crk can interact directly with tyrosine kinase receptors. For example, direct recruitment of Crk to the PDGFRα  or the VEGFR-3  can regulate immediate post-receptor signals from a subset of growth factors at the plasma membrane.
Once the Crk proteins are engaged by tyrosine phopshorylation via the SH2 domain in a particular time and place, they can transmit signals downstream through constitutive binding to the Crk SH3 domain. Although many Crk SH3N binding proteins have been identified and have been the subject of an excellent review , some of the better characterized p130Cas signaling pathways are described below.
Crk and C3G
C3G (C rk SH3-domain-binding g uanine-nucleotide releasing factor) was isolated in a screen for Crk SH3N-binding partners and was the first protein identified that bound to the SH3 domain of Crk [114, 115]. In addition to several proline-rich sequences that bind Crk, C3G has a guanine nucleotide releasing activity that removes GDP from the small GTPases Rap1 (Krev-1), Rap-2, and R-Ras, allowing their spontaneous reloading with GTP . Rap-GTPases (like other Ras-GTPases) are thought to be inactive when bound to guanosine diphosphate (GDP), and activated when bound to guanosine triphosphate (GTP). As their name implies, GTPases possess intrinsic enzymatic activity that hydrolyses GTP to GDP and phosphate. Thus, upon binding to GTP, the duration of Rap-GTPase activity depends on the rate of hydrolysis. C3G (and other guanine nucleotide exchange factors) act by binding Rap-GTPases and catalyzing the release of their bound GDP nucleotide. Once released from C3G, the Ras-GTPase quickly binds fresh guanine nucleotide from the cytosol because cytosolic GTP is approximately ten times more abundant than cytosolic GDP.
Although Rap1 have strong homology to classic Ras proteins and were initially identified as native antagonists to Ras that bound to and inhibited Raf-1, Rap1 signaling is clearly complex and multifactorial, and targets of Rap-1 have been implicated in cell proliferation, cytoskeletal reorganization during cell adhesion to ECM, and for cell-to-cell contact . Although expression of "activated" Rap1V12 in fibroblasts does not induce classic cell transformation analogous to RasV12, Rap1 has been implicated in increased cell proliferation and transformation in EGF-stimulated Swiss 3T3 fibroblasts that express B-Raf, and these cells can form tumors in nude mice [117, 118]. In addition, studies in both mice and humans have demonstrated an important role for the RapGAP, Spa-1, in cancer development. For example, Spa (-/-) targeted mice show constitutive activation of endogenous Rap1 in hematopioetic progenitor cells, and these mice exhibit a marked increase in granulocytic cells analogous to CML, with a subset showing blast crisis analogous to CML . Spa-1 polymorphisms that alter Spa-1 activity have also been correlated with a likelihood for metastastic disease in humans . In addition to Spa-1 deficiency, forced expression of C3G has been implicated in T-cell acute lymphoblastic leukemia . C3G also has been implicated in the activation of R-Ras, which can activate the stress-related kinase JNK1 [122, 123]. Although the catalytic activity of C3G is involved in transformation in rodent fibroblasts, interestingly, sequences outside the catalytic domain of C3G also have tumor suppressor activity, and recently PP2A was shown to bind to the N-terminal region of C3G [124, 125]. Finally, in chronic myelogenous leukemia (CML), a novel truncated form of C3G (p87), without the inhibitory region, was found to be associated with Bcr-Abl .
One of the most important biological effects of Rap1 signaling is the acute regulation of cell adhesion, in part, mediated by the activation of integrins. Recruitment of C3G to the plasma membrane links p130cas to C3G, where Rap1-GTP increases the affinity of β1 integrins to ECM, the so-called inside to outside integrin signaling [127, 128]. Rap1 signaling also induces cell spreading and F-actin dynamics by binding to the Rap1-interacting adaptor molecule (RIAM) , which may also control E-cadherin and cell to cell interactions . Functionally, C3G activity is potentiated by tyrosine phosphorylation at Tyr504 , mediated by SFKs, and therefore it is likely that C3G activity coincides with its recruitment to p130Cas and the activation of Src that accompany cell adhesion to ECM, following stimulation of cells with certain cytokines, as well as following stretch-inducible mechanical force. Tyrosine phosphorylation of C3G is further accompanied by its relocation from the cytosol to the cortical actin cytoskeleton and the trans-Golgi, additionally suggesting a role in protein trafficking or secretion . In neuronal cells, one of the best-understood functions of p130Cas/Crk/C3G-induced activation of Rap1 involves the activation of B-Raf in neurons. B-Raf forms a stable complex with Rap1 and converges on a signaling pathway to sustainably activate the mitogen-activated protein kinase (MAPK) pathway to control neuroblast differentiation .
Crk and Son of Sevenless (SOS)
SOS was first identified in Drosophila melanogaster as a functional gene product downstream of sevenless in the Ras/MAP kinase pathway . When sevenless is mutated during development of the fly's ultraviolet light-sensitive compound eye, the seventh, central photoreceptor (R7) of each ommatidium fails to form. Subsequent studies indicated that SOS was homologous to the yeast exchange factor for Ras, CDC25, and then identified as a central conduit to link receptor tyrosine kinases to Ras following growth factor stimulation in metazoan cells . In mammalian cells, there are two SOS homologs, SOS1 (~170 kDa) and SOS2 (~150 kDa) derived from different genetic loci. The N-terminal of SOS1 encodes a Dbl-homology (Dbl) and Pleckstrin homology (PH) duet that exchanges GTP for GDP on Ras, as well as tandem C-terminal proline-rich motifs that interact with several adaptor proteins, including Grb2 and E3b1. Although SOS can interact with both Grb2 and E3b1 (a Rac1 GEF) adaptors simultaneously, very recent studies indicate that the timing of these reactions significantly differ in vivo, the Ras activation occurring in a rapid and transient fashion, while Rac1 activation occurring in a more sustained and protracted manner .
Both SOS1 and SOS2 possess repetitive proline motifs that conform to consensus Crk SH3 binding motifs and direct stable association between Crk and SOS. A functional significance for this interaction is evident from co-expression studies showing that dominant negative Ras protein suppresses v-Crk-induced cell transformation in CEFs . Moreover, in Crk-I transformed NIH3T3 cells, RNAi downmodulation of SOS1 significantly decreased cell transformation (soft colony formation) and tumorigenicity when Crk-I transformed cells were xenografted in nude mice (Bruce Mayer, personal communication). Finally, it is also interesting to point out that the major deficiencies observed in Crk null mice (namely cardiovascular and cranial defects) are also observed in subsets of patients with Noonan Syndrome. Noonan syndrome is a congenital syndrome characteristic of multiple abnormalities that include facial dysmorphology and cleft palate, short stature, and hypertrophic cardiomyopathic defects that include defects in the vascular smooth muscle architecture . In about 15–20% of the clinical cases of Noonan syndrome, SOS1 mutations are present throughout the coding region of the gene that directly influence the GEF activity . Future studies aimed at crossing SOS1 mutant with Crk (-/-) and Crk transgenic mice may shed additional light on how Crk and SOS interact with respect to tissue-specific functions.
Crk and DOCK180 (DOCK1)
DOCK180 (Do wnstream of C rk; DOCK1) was originally cloned by far-Western blotting, with Crk SH3N domain as a "bait" molecule [139, 140], and represents the third GEF that directly associates with the Crk SH3 domain. However, unlike SOS and C3G exchange factors, DOCK1 does not contain the conventional DH-PH duets known to promote nucleotide exchange. Instead, DOCK1 mediates nucleotide exchange via a novel evolutionarily conserved Docker/DHR2/CZH2 domain that recruits ELMO, which functions in trans as a bipartite Rac-1-GEF [141, 142]. Although a complete understanding of the structural mechanisms for Rac1 activation are still emerging, biochemical studies indicate that DOCK1 must interact with ELMO for efficient Rac1 activation. The prototypical member of the ELMO family is ELMO1 that is characterized by N-terminal Armidillo repeats, and a PH domain and proline-rich sequences towards the C-terminus.
The bi-molecular nature of the DOCK1/ELMO Rac-GEF argues that, in contrast to conventional Rac-GEFs in which the DH and PH domains act as cis-acting elements, a significant aspect of the regulation of DOCK/ELMO mediated catalysis depends on the regulation of the association between DOCK1 and ELMO proteins, as well as other trans-acting factors (Figure 3B). In this respect, there are at least three known mechanisms by which DOCK1 and ELMO interact, one involving a PxxP-dependent SH3 domain interaction between the C-terminus of ELMO and the SH3 domain of DOCK1, the second involves atypical elements within the C-terminus of ELMO that interact with non-proline rich motifs in the N-terminus of DOCK1, and third, the ELMO PH domain interacts with nucleotide-free Rac1. Recent evidence suggests that several (if not all DOCK family proteins) have the capacity for structural inhibition mediated by an atypical SH3 domain interaction between the N-terminal SH3 domain and the DHR2 region . Binding of ELMO to the SH3 domain of DOCK1 would not only relieve the inhibition of DHR2 and permit Rac1 to bind to this region, but may also permit PH mediated catalysis. Therefore, one of the important strategies to identify the relevance of DOCK180 and ELMO is to recognize the relevant upstream and downstream effectors. A clue into such a pathway was shown in recent studies that tyrosine phosphorylation of ELMO (by Src family kinase) is required for Rac-GEF function. In addition, recent studies indicate that a newly characterized phosphatidylserine receptor called BAI can directly interact with DOCK1 , and the RhoG GEF Trio can directly interact with ELMO . Finally, binding of ELMO to DOCK1 inhibits the ubiquitylation and degradation of DOCK1 , suggesting that DOCK proteins may also be regulated by protein stability.
Despite the fact that DOCK1 was identified by virtue of its direct interaction with Crk, it is still not entirely clear whether Crk binding to DOCK1 directly mediates Rac1 activation at the biochemical level [60, 146]. In this respect, Crk appears to have a secondary function to regulate Rac1 activation  mediated by the SH3C domain . Mutations in the SH3C domain of Crk prevent the regulated turnover of the DOCK1/ELMO complex, suggesting that Crk also controls assembly of the ELMO/DOCK1 complex. Despite these findings, more recent functional studies employing LR73 fibroblasts showed that a direct interaction of Crk II with DOCK1 was dispensable for both engulfment of apoptotic cells as well as for the recruitment of DOCK1 since expression of mutant DOCK1 proteins that lack the proline-rich sequences were sufficient for supporting Rac1 activation . This result is interesting in that it contrasts earlier genetic studies identifying Crk II (ced-2) in a linear pathway with DOCK1 (ced-5) (see below) for motility and for engulfing apoptotic corpses. Moreover, several studies have shown that dominant negative Crk proteins block migration towards certain ligands. For example, A549 lung adenocarcinoma cells and ECV304 endothelial-derived cells adhering to laminin-10/11 show more robust Rac1 activation compared to these same cells on vitronectin, and further dominant negative Crk proteins that don't interact with DOCK1 block Rac1 activation . Taken together, these data imply that there may be Crk/DOCK dependent and Crk/DOCK independent interactions that depend on the initiating ligands and upstream tyrosine kinases that become activated.
Studies over the past several years have indicated that DOCK180/DOCK1 is the prototype of a superfamily of at least 11 homologous members divided into 4 subfamilies based on conservation in the DHR1/CZH1 and DHR2/CZH2 domains as well as their specificity towards Rac1 or Cdc42 [149, 150]. This larger family, called CZH [CDM (CED/DOCK/Myoblast City)-Zizimin homology] can be further divided into either DOCK related (specific for Rac1) or Zizimin related (specific for Cdc42)  (Figure 3A). In terms of relevance to Crk and CrkL, to date none of the zizimin proteins possess classic PxxPxK, R motifs that bind Crk SH3 domains, and of the DOCK families, DOCKA (which consists of DOCK1, DOCK2, and DOCK5), and DOCKB (which consists of DOCK4 and DOCK3/MOCA), only DOCK1 has conventional Crk SH3 binding sites. However, very recent studies indicate that DOCK5 participates in Caco-2 cell motility by binding Crk L and Crk II SH3 domains via atypical proline-rich motifs in their carboxyl-termini . Similar interactions between DOCK and Crk proteins outside of the classical PxxPxK motifs have been reported for DOCK2, which lacks the classic C-terminal region for binding Crk, and appears to bind CrkL via regions of the DOCKER domain . Recent studies also suggest that DOCK5 cooperates with DOCK1 for the differentiation of myofibers , and clearly, crossing these mice with Crk II (-/-) or CrkL (-/-) mice should better define the level of functional interactions. If this is generally true, then the role of Crk signaling in regulating DOCK-C and DOCK-D family members should be revisited.
Crk and JNK
In addition to the ability of Crk to activate JNK through C3G and R-Ras noted above, Crk II can also interact with JNK directly through a proline-rich sequence in JNK and the SH3N domain of Crk . Similar to the case for C3G and DOCK180, recruitment of a Crk/JNK complex to p130Cas may result in the localization to JNK to its relevant upstream kinases, such as MKK4 and HPK-1 . A direct interaction between Crk and JNK may indeed be biologically relevant, since studies by Girardin and Yaniv have shown that a JNK mutant (K340A) that fails to bind Crk is also defective in EGFR signaling and Rac1 activation . These studies also suggest significant crosstalk between JNK and Rac1, and the involvement of DOCK180 in this pathway should be investigated. These results may offer an explanation for the observations that integrins regulate progression through the G1 phase of the cell cycle in a JNK-dependent manner . Following integrin engagement, JNK activation requires association of FAK with a Src kinase and p130Cas, the phosphorylation of p130Cas, and subsequently, the recruitment of Crk. FAK-JNK signaling appears to be necessary for proper progression through the G1 phase of the cell cycle. These findings suggest a role for p130Cas and Crk in both the activation of JNK and control of the cell cycle, as well as identification of a physiological stimulus for JNK signaling that is consistent with the role of Jun in both proliferation and transformation. There also appears to be significant crosstalk between these pathways. Finally, in Drosophila melanogaster, downstream of platelet-derived growth factor receptor (PDGFR) or vascular-endothelial growth factor receptor (VEGFR), Crk/DOCK180/ELMO/Rac interactions mediate JNK activation, leading to dorsal closure .
Crk and Abl
Abl is a non-receptor tyrosine kinase first identified as the cellular homolog of the v-Abl oncogene product of the Abelson murine leukemia virus, and has multiple roles in cell growth, transformation, cell stress, apoptosis, and remodeling of the actin cytoskeleton. Arg (Abl-related gene) is over 70% homologous to Abl, and both proteins contain four tandem proline-rich motifs located just C-terminal to the kinase domain [10, 158] that mediate binding to the N-terminal Crk SH3 domain. Systematic mutagenesis studies whereby each of the four proline-rich motifs was assigned function and SH3 domain specificity showed that motifs 1, 2, and 4 (from the N-terminus to the C-terminus) predominantly bound Crk, whereas motif 3 was more promiscuous and bound SH3 domains of Nck, Ponson/CAP, and ArgB2 . Moreover, in reconstitution studies in NIH3T3 cells, it was further shown that binding of Crk and Nck promoted distinct morphological phenotypes upon adhesion to ECM; Crk stimulated lamellipodia formation (via Rac1) while Nck stimulated filopodia formation (via Cdc42). This suggests that binding of Crk to the Abl proline-rich motifs is balanced to fine-tune actin-based membrane dynamics as cells adapt to their surrounding environment. This is likely functionally important for integrin function, as cell adhesion and integrin ligation induces p130Cas tyrosine phosphorylation and the translocation of Abl from the nucleus to focal adhesions, where focal adhesion-associated Abl contributes to adhesion-dependent actin reorganization and membrane ruffling [160, 161]. One of the critical functions of Abl in focal adhesions is to regulate the formation and maintenance of adherens junctions via a Crk and Rac1 pathway that regulates cadherin-catenin adhesion complex . Additionally, Abl has been shown to tyrosine phosphorylate SOS1, which associates with Abl most likely through Crk . This might explain why Rac1 is constitutively activated in Bcr-Abl transformed cells, whereby Rac1 is implicated in increased motility of the cancer cells.
In general, the interaction between Abl and Crk is suggested to be bi-directional, meaning that Crk can both transactivate Abl and Abl can transinhibit Crk II and CrkL. Transactivation of Abl by Crk II was first reported by Shishido and Hanafusa based on observations that co-expresion of Crk and Abl increased the net tyrosine phosphorylation of cellular proteins in Abl-expressing cells (Figure 4A). Subsequently, Reichman et al showed that Crk expression with Abl induced tyrosine phosphorylation of the major autophosphorylation sites in Abl (Tyr 245 and Tyr 412), suggesting that Crk binding to Abl directly enhances Abl enzymatic activity. Consistent with this idea, Crk transactivation is much more efficient when either the Y221 or the P225 motifs in Crk are substituted to prevent Crk phosphorylation and intramolecular binding to its own SH2 domain [164, 165]. Although structural analysis is warranted to identify specific features of Abl transactivation, biochemical studies indicated that transactivation is mediated by the SH3 linker in Crk as well as the PNAY motif in the RT-loop of the C-terminal SH3 domain. It will also be important to decipher whether Crk mediated transactivation induces phosphorylation on specific substrates, as this would help elucidate functional significance. Biochemical and cell biological studies also suggest that Crk binding to Abl causes an increase in the steady state levels of Abl , possibly to prevent degradation. Abl is known to be degraded by either ubiquitin or caspase-mediated pathways. In vitro, addition of the Crk SH3N domain can reverse the inhibition of Abl activity by F-actin, further suggesting that Crk transactivation may occur locally in the focal adhesions .
Subsequent to the binding and transactivation of Abl by Crk, Crk Y221 is itself an excellent substrate for Abl, and binding of Crk to Abl reflects a potent downmodulatory signal . However, while phosphorylation of Tyr221 may be the ultimate fate for the Crk/Abl interaction, few studies have attempted to understand in detail what happens between the initial binding of Crk to Abl and the ensuing Abl-mediated Crk phosphorylation. This should be an important avenue of future investigation with goals to identify transition state conformers of Abl and Crk, as well as, to attempt to generate various activation state mutants of Crk or Abl that reflect these transition states. For example, in rodent and human Crk, binding of Crk to Abl causes conformational changes that expose a PPPP motif in the Crk SH2 domain that in turn binds the SH3 domain of Abl to stabilize the Abl/Crk interaction [167, 168]. Moreover, virtually nothing is known about how Crk binding to Abl influences the C-terminal region of Abl, including the ability of Abl to bind F-actin or to DNA. Clearly, one important function of Y221 phoshorylation is to prevent unregulated transactivation of complexes involving Crk and Abl. However, physiologically, it is more likely that the dynamic state of activation and deactivation of Abl by Crk represents another example of dynamic regulation to fine-tune signals related to Rac1 activation, actomyosin plasticity, and motility.
Tyrosine phosphorylation of Crk on Y221 is also not of inconsequence with respect to signal transduction pathways initiated by integrins and growth factors that led to p130Cas phosphorylation. Klemke and colleagues were the first to demonstrate that Abl inducible Crk Y221 phosphorylation acted as a molecular switch to dissociate a p130Cas/Crk/DOCK180 ternary complex, in order to block motility and invasive behavior of human pancreatic cancer cells  (Figure 4B). More recently, a similar negative regulatory network has been reported for a p130Cas/Crk/C3G ternary complex, whereby Abl promotes Crk Y221 phosphorylation and disrupts the interaction between Crk and C3G. This subsequently leads to decreased activation of Rap1-GTP, decreased affinity of β1 integrin to ECM, and loss of cell adhesion . Abl also appears to have a negative regulatory role in HGF-stimulated hepatocytes, where Crk Y221 phosphorylation inhibits complex formation between Crk and Cbl . Interestingly, in tumor cells tyrosine phosphorylation of Crk by Abl may underscore a novel anti-oncogenic pathway. For example, Noren et al have shown that EphB2 receptor tyrosine kinase acts as a tumor suppressor in breast cancer cells and that depletion of EphB2 or its ligand ephrinB2 prediposes breast cancer cells to invasion and metastasis . These investigators further found that ephrinB2 induces Crk Y221 phosphorylation and decreased Rac1 activation. While this indicates that under some conditions Abl-mediated Crk phosphorylation may inhibit oncogenicity, it does raise a paradox as to whether Imatinib (Gleevec), may have side effects when used to treat CML.
Finally, a very interesting study by Peterson and Long identified an important functional role in inhibitory signaling downstream of the human killer cell Ig-like receptor (KIR) that inhibits Natural Killer (NK) cell activation and cytotoxicity . Using a clever experimental system whereby NK cells either activate or inhibit target cell killing through a HLA class I receptor, these investigators determined that the killing phenotype correlated with the assemblage of a Cbl/Crk/C3G complex, whereas the inhibitory phenotype correlated with the formation of a complex with Abl and Crk, and the phosphorylation of Crk to disassemble the aforementioned complex with C3G . This study defines an unusual role for Abl and Crk assemblages in the signaling of inhibitory receptors.
Recent studies also suggest that pY221Crk is not necessarily a biologically inert species. Interesting work by Vuori and colleagues showed that pY221 Crk was required for recruiting activated Rac1 to the membrane fraction, and Rac1 re-localization was prevented by expression of the Y221F Crk mutant . Most interestingly, targeting of CrkY221F to the plasma membrane by fusing a CAAX box rescued the ability of Crk to localize Rac1 and promote laemellipodial formation and migration. Further support of a dynamic, rather than a static, role of Crk Y221 phosphorylation came from studies showing that Y221F Crk II expression prevents regulated turnover of Crk/Abl/p130Cas or Crk/Abl/paxillin complexes, leading to impaired adhesion and migration . Taken together, these data suggest that regulated phosphorylation of CrkY221 by Abl and Arg are not a simple on-and-off mechanism, but rather a dynamic process to execute Crk-dependent signaling involving Rac1 activation and cell migration.
Regulatory role of the C-terminal region of Crk II and domain-domain communication
Since the discovery of v-Crk and c-Crk II, it is clear that the C-terminal region exerts negative regulation on Crk signaling and transformation. Whereas models for protein interactions mediated by the SH2 and SH3N have been well documented to explain how Crk functions as an adaptor protein [14, 168, 174], how the C-terminus of Crk imposes negative regulation has only recently begun to be unraveled [15, 175–178]. Earlier biochemical studies suggested that the Crk SH3C could impose regulation on the adaptor protein function of Crk. For example, when expressed in Rat 3Y1 cells, the SH3C domain suppressed p130Cas phosphorylation and the transforming potential of Crk II, and negatively regulated EGF signaling to Ras . Mutations in the SH3C of Crk also lead to increased association of Abl with the SH3N, suggesting that the SH3N and SH3C functionally interact . This latter study also revealed that disruption of the boundary between the SH3 linker and the SH3C domain (aa 240–296 in chicken Crk) regulated the activation of FAK and increased the numbers of focal adhesions in cells. This paradigm for autoinhibition of the SH3 linker and SH3C domain suggested that regions independent of the Y221 might contribute to the negative regulation of Crk.
NMR studies on various Crk proteins that contain the SH3C domain and SH3 linker have begun to offer explanations as to the nature of its regulatory aspects. Like the Y221YAQP motif, the SH3C contributes to the negative regulation of Crk II and CrkL by intramolecular domain crosstalk. Although the Crk SH3C is highly conserved from C. elegans to mammals, there are notable differences in the Crk SH3C compared to canonical SH3 domains that bind PxxP motifs. First, although the amino acids that comprise the structural elements of the hydrophobic core are highly conserved, the amino acids that comprise the surface of the SH3C, in particular, where the PxxPxK is expected to bind, are highly divergent [165, 176]. The charged residues on the surface of the SH3 domain that interact with the P1, P3, and K6 residues are replaced by unusually polar and bulky amino acids, precluding a socket structure for the prolines and providing a clear explanation for why CrkSH3C does not bind PxxPx(K, R) ligands. Indeed, inspection of the solution NMR structure of the isolated murine SH3C does not show an obvious binding grove, suggesting that this domain may not bind to, or bind with low affinity, to target proteins . Indeed, the only known protein that has been shown to interact with the Crk SH3C domain is the nuclear export protein Crm1, which is posited to bind to a LALEVGELVKV sequence in the Crk SH3C, and has broad similarity to a nuclear export sequence (NES) . However, in the monomer Crk proteins, the LVK motif is buried in the hydrophobic core of the SH3C and not likely to form a contact surface. Interestingly, in the case of the CrkL SH3C, partial SH3 unfolding that may occur during monomer to dimer transition in the nucleus, specifically may expose this LVK sequence for Crm1 binding . This suggests that, in the context of nuclear Crk, regulated unfolding of the SH3C domain may provide a novel and unexpected regulatory mechanism for Crk efflux. Future studies aimed at identification of the molecular mechanisms of Crk unfolding, and whether this reflects an anti-apoptotic mechanism warrant further investigation .
To better understand the mechanism by which the SH3C and linker regulate the SH3N, Kalodimos and coworkers used NMR analysis on a SH3N-linker-SH3C fragment of chicken Crk  (Figure 5). In solution at pH 5.5, this fragment showed characteristics of two species in slow exchange originating from cis-trans isomerization at the proline from the PFY motif at the boundary of the linker-SH3C, a region previously identified as an important negative regulatory region in chicken Crk . Moreover, the cis and trans forms appear to have entirely different regulatory functions: the cis isomer is locked in a configuration that tethers the SH3N, preventing ligand binding, while the trans configuration appears extended and flexible (Figure 5). Support of this model comes from the fact that mutation at either Pro238 or F239 abrogates cis-trans isomerization, favoring the trans form and leading to increased association of Abl with the SH3N . By sequence analysis, this proline was expected to be a substrate for Cyclophilin A (CypA), a protein that has peptidyl prolyl isomerase activity and catalyzes the prolyl isomerization of peptide bonds. Interestingly, this suggests that enzymatic-catalyzed cis-trans proyl isomerization may influence Abl and Arg dependent phosphorylation and hence the rate at which Crk proteins are negatively regulated. Future studies should investigate the physiological significance of CypA on Crk function, as well as whether cyclosporin A (inhibitor of CypA) may be a specific inhibitor of Crk pathways in vivo. Another interesting aspect of this regulatory mechanism involving the PFY motif in chicken Crk is that F239, adjacent to P238, was absolutely required to achieve cis-trans isomerization, and mutation of F239 to Ile or Val abrogated this regulatory mechanism. This is most intriguing, given the fact that chicken is the only species of Crk with the PFY motif. This motif diverges to PIY in human and PVY in mouse (Figure 6).
Recently, Inagaki and colleagues performed NMR of the entire structure of Crk I and Crk II and the phosphorylated form of Crk II without the SH3C (amino acids 1–228)  (Figure 7A). Interestingly, while the SH2 and SH3 domains of Crk I are flexible , conversely, Crk II forms a compact structure. Solution structure of Crk II showed that the three SH domains were assembled to a central short sequence, residing in the inter SH3 region (amino acids 224–237) called the inter SH3 core region (ISC), and that this compact structure was stabilized mainly by the SH3C domain. These studies also found that the SH3N is semi-closed and covered by the bottom of the SH2 domain, mimicking the interaction of SH3 and PxxPxK. These structures in many respects provide confirmation of previously observed differences in the biological activity of Crk I versus Crk II, including the fact that the affinity of Crk II for the SH3N target polyproline motifs is lower than the affinity of Crk I. Upon phosphorylation, the entire Crk structure is dramatically shifted, and the SH2 binds to phospho-Y221, as reported previously. In phosphorylated Crk II, the SH3N surface was shown to be blocked by an internal sequence between the SH2 and SH3 domains (Arg122- Glu133), suggesting that phosphorylated Crk II cannot bind to either of the SH2 or SH3N targets (Figure 7A). Again, these observations are consistent with previous biochemical and cell biological studies, which predict that: (i) Crk I is constitutively active, (ii) Crk II is regulated by the intramolecular SH3N interaction, and (iii) phosphorylated Crk appears to be completely shut off for binding effector proteins (Figure 7B and additional File 1). Moreover, although details remain to be determined, it appears that the mode of negative regulation for mammalian and chicken Crk II proteins has somewhat diverged.
Complex Cell Biological Processes regulated by Crk II
Role of Crk in Rac1 activation, cell migration, and phagocytosis
A significant advance towards a molecular understanding of Crk biology was revealed following a screen for genes involved in cell-death pathways in C. elegans using classical worm genetics . Earlier studies in C. elegans elegantly identified genes that mediated the manifestations of apoptosis (Ced-3/Caspases and Ced-4/APAF-1) and in the suppression of apoptosis (Ced-9/Bcl-2) [183, 184]. Upon closer examination of the worm phenotypes, a third class of mutations was found, based on their inability to engulf apoptotic corpses, and as such gave a phenotype of persistent corpses. Four of these gene products, representing the dominant pathway for clearance in the worm, were identified as a module consisting of Ced-2, Ced-5, Ced-10, and Ced-12, which have the mammalian counterparts, Crk II, DOCK180, Rac1, and ELMO, respectively . In addition to the defects in cell clearance in the C. elegans model, a second related, if not overlapping, phenotype was noted in the Ced-2/Crk deficient worms that involved defects in the migration of cells into the distal tip . Defects in distal tip migration could be rescued by Ced10 (Rac1) or Ced5 (DOCK180), suggesting that Crk was positioned upstream of Rac1 in this pathway. Investigations by Ishimaru and Hanafusa, studying Drosophila metamorphosis, elegantly demonstrated that Drosophila Crk (Dcrk) cooperates with DOCK180 (called myoblast city or Mbc) and ELMO for border cell migration in oogenesis and hemocyte migration during embryogenesis, and further mapped this pathway downstream of PVR, the fly equivalent molecule of PDGF Receptor and VEGF-Receptor . In addition to these functions in physiological cell migration, Crk also appears to utilize cell migration and motile responses in pathophysiological functions that include pathogen uptake and virulence, as well as cancer metastasis.
Crk and bacterial infectious diseases
In recent years, a growing and unexpected body of evidence suggests that Crk may contribute to bacterial pathogenesis by influencing entry into cells or by serving as targets of bacterial toxins that disrupt essential cellular function through Crk-dependent mechanisms. A role for Crk in bacterial uptake pathways was first noted by Weidow and colleagues, who demonstrated that Yersinia pseudotuberculosis infection into human epithelial cells activated the p130Cas-Crk-DOCK180-Rac1 pathway via binding of the bacterial invasin to the β1 integrin receptor [63, 186]. This pathway appears be somewhat analogous to the phagocytic pathway for apoptotic cells, although the phagocytic pathway involves β3 and β5 integrins. More recent studies by Burton and colleagues found that Crk is also implicated in Shigella flexneri infection into NIH 3T3 cells using a curious pathway involving Abl-Arg-mediated Crk Y221 phosphorylation, whereby Crk Y221F mutation blocked S. Flexneri infection . In very provocative studies involving Pseudomonas aeruginosa infection  and Helicobacter pylori infection , Crk appears to acquire a gain-of-function activity by co-opting activities with bacterial virulence factors. In the former study, a novel role for Abl and Crk phosphorylation was identified and found to be essential for Pseudomonas aeruginosa internalization . In the case of H. pylori, Crk associates with tyrosine phosphorylated CagA, and the CagA/Crk complex regulates Erk signaling and Rac1-mediated cytoskeletal assemblages . Although in H. pylori-associated gastric cancer, CagA-SHP2 signaling is known to play a central role, the functional significance of the CagA/Crk complex for cellular infection should be evaluated. Finally, exoenzyme T (ExoT) of P. aeruginosa ADP-ribosylates Crk I and Crk II at specific residues in the SH2 domain (Arg20) that impairs binding of Crk to p130Cas . Although to date, there has been very little to report on the role that Crk proteins play in viral entry and pathogenesis, this may be an area of active ferment as a recent study showed that CrkL can bind to NS1 proteins in Influenza A infected cells .
Crk and human cancers
A number of studies have suggested that Crk may play an important role in human cancers. Immunohistochemical analysis of human cancers showed the over-expression of Crk in adenocarcinomas of lung, breast, and stomach, and also sarcomas, followed by the demonstration of increased levels of Crk mRNA in more aggressive phenotypes of lung adenocarcinoma [62, 190–194]. In an analysis of gene-expression profiles of 86 primary lung adenocarcinomas, increased Crk expression was shown to be a predictive factor, contributing to poor prognosis and shorter survival . Overexpression of Crk in tumor cells or in experimental model systems leads to increases in tyrosine phosphorylation of p130Cas and the activation of an intracellular loop that further enhances the activity of Crk and induces increased motility and the aggressive potential of cancer cells. Therefore, Crk proteins are not simply conduits for intracellular signal transduction but also can control the amplitude of signaling.
Based on these observations, several groups have begun to investigate the effects of Crk knockdown on the phenotypic behavior of human tumor cells. Simultaneous downregulation of both Crk I and Crk II by siRNA demonstrated the essential role of Crk in the malignant features of human ovarian cancer cells , synovial sarcoma cells , and brain tumors, such as glioblastoma cells . Recent studies also suggest that Crk II is a likely target for MiR-126, and that overexpression of MiR-126 in lung cancer cells decreased adhesion, spreading, and invasion . In Crk knockdown cells, formation of focal adhesion was decreased, and cells had cytoskeletal disorganization, whereby formation of laemellipodial structures at the leading edges of cells was impaired (Figure 8, additional file 2 and additional file 3). For example, knockdown of Crk in human synovial sarcoma cells showed a suppression of HGF-dependent activation of Rac1 and decreased motility . In glioblastoma cells, Crk knockdown affected the early attachment to laminin. In addition, Crk also regulated Rho-dependent phosphorylation of ezrin-radixin-moesin proteins that direct migration towards hyaluronic acid in the brain . Increased levels of Crk I mRNA are frequently observed in WHO grade III and IV malignant gliomas [191, 193]. Thus, Crk I expression may correlate with poor prognosis, and this is a novel example of splicing-dependent control of human cancer malignancy. As Crk knockdown in cell lines was not lethal, Crk may be an effective therapeutic target for decreasing the metastatic potential of human tumor cells (see additional file 2 and additional file 3).
The above studies suggest that Crk may have a central role in cell motility and metastasis in highly aggressive motile cancer cells. There are a plethora of studies showing that p130Cas-HEF and the FAK-Src circuitry is involved in tumorigenesis, and Crk may indeed be an attractive target due to its central integrative downstream role in signaling by these molecules. Recent studies now suggest that DOCK180 and/or ELMO may be oncogenes, or have oncogenic potential, and Crk may be an attractive drug target in tumor cells that use Rac1 activation to acquire motile or metastatic potential. DOCK180, for example, is overexpressed at the borders, but not at the centers, of human malignant glioma, and together with ELMO1, it regulates Rac activity to enhance tumor invasion . Although the relation of C3G and human cancer is controversial, an increase in C3G expression is observed in human small cell carcinoma , and a Crk-C3G-Rap1 pathway leading to B-RAF and Erk activation is involved in transformation downstream of Ret in papillary thyroid carcinoma . In addition, C3G activation of Rap1 regulates a number of cell-matrix or cell-cell interactions through integrins and cadherins , pointing to a role for C3G overexpression in the modulation of adherens junctions, leading to tumor cell dissemination [130, 198]. It will be important to experimentally observe whether siRNA toward Crk can influence the motile behavior of cells that express upregulated C3G and/or Rap-1. Finally, a most curious function for CrkL has been recently proposed, whereby a secreted form of CrkL was shown to bind to the plexin-semaphorin-integrin (PSI) domain of β1 integrin at the extracellular domain in order to promote cell growth and survival . These authors made the provocative suggestion that targeting extracellular Crk proteins may have therapeutic value.
Another area of clinical investigation is the role of tyrosine phosphorylation of CrkL and Crk II in CML [199–201]. This cancer results from the chromosomal translocation termed Philadelphia chromosome (Ph1) as t(9;22), generating the aberrant chimeric protein Bcr-Abl. Current understanding is that CrkL is not essential for the development of CML, because CrkL knockout mice developed CML by the introduction of Bcr-Abl. However, the full role of CrkL and Crk II in CML has yet to be explained.
Crk and pro-apoptotic pathways
In recent years, both Crk II and CrkL have been implicated in nuclear signal transduction events. For CrkL, elegant studies have shown that CrkL binds tyrosine phosphorylated Stat5 in Bcr-Abl expressing cells, and in a variety of cytokine-stimulated hematopoietic cells [202, 203]. In many, if not all, of these cases, the complex translocates to the nucleus to bind STAT5-responsive elements [204, 205]. Although Crk II can also bind to STAT5, this complex does not appear to enter the nucleus, nor do anti-Crk II antisera supershift STAT5 DNA binding complexes . While these studies may indicate that CrkL has a role in transcription of interferon regulated genes, interesting studies by Kornbluth and colleagues indicate that Crk may actively and directly participate in apoptosis in Xenopus by activating caspases . Depletion of Crk II from egg extracts prevented apoptosis, and these studies further demonstrated that Crk binds to the cell cycle regulatory protein Wee1, implying that this pro-apoptotic function of Crk II may be due to nuclear localization . Evidence in support of this model includes the fact that Crk has a putative NES in the SH3C domain, that when mutated increases cell death, and that ionizing radiation increases complex formation between Wee1 and Crk II. Furthermore, evidence for a direct role of Crk in apoptosis is supported by the findings that specific targeting of Crk to the nucleus by fusing NLS sequences, spontaneously activates apoptosis and potentiates stimuli that induce apoptosis . Recent structural studies with CrkL indicate that the Crk NES may only function in the dimeric state of the protein, mediated by a homotypic interaction in the SH3C domains . These studies predict that the Crk NES motif may become exposed conditionally under certain circumstances, an event that would actively promote efflux of a nuclear Crk pool into the cytoplasm. However, the presence of an NES sequence in Crk II does not explain how Crk II initially trafficks to the nucleus, since Crk does not appear to possess any NLS sequences. It will be equally important to characterize the physiological signals that result in Crk translocation into the nucleus, and whether there exists a regulatory signal that controls the interaction between Crk and Crm1.
Future directions and new frontiers
The Crk gene was identified about 20 years ago, and the characterization of Crk and its mode of cell signaling has arguably revolutionized the way we think about intracellular signal transduction. Despite significant advances and an enormous wealth of new information, the field is now faced with the challenging problem of how these complex signaling networks are controlled in time and space to produce biological responses. Efforts will need to be redirected from the relatively simple task of identifying specific effector proteins to an understanding of when and where these complexes are formed in cells. Development of FRET technology that reports specific protein-protein interactions, as well as the generation of a battery of phosphospecific antiseras against specific post-translational epitopes, should help provide a better understanding of Crk biology. This exciting field can expect substantial progress as the new observations continue. One thing is certain: the field of Crk biology will continue to grow over the next 20 years.
Mayer BJ, Hamaguchi M, Hanafusa H: A novel viral oncogene with structural similarity to phospholipase C. Nature. 1988, 332: 272-275.
Mayer BJ, Hamaguchi M, Hanafusa H: Characterization of p47gag-crk, a novel oncogene product with sequence similarity to a putative modulatory domain of protein-tyrosine kinases and phospholipase C. Cold Spring Harb Symp Quant Biol. 1988, 53 (Pt 2): 907-914.
Tsuchie H, Chang CH, Yoshida M, Vogt PK: A newly isolated avian sarcoma virus, ASV-1, carries the crk oncogene. Oncogene. 1989, 4: 1281-1284.
Matsuda M, Mayer BJ, Hanafusa H: Identification of domains of the v-crk oncogene product sufficient for association with phosphotyrosine-containing proteins. Mol Cell Biol. 1991, 11: 1607-1613.
Matsuda M, Mayer BJ, Fukui Y, Hanafusa H: Binding of transforming protein, P47gag-crk, to a broad range of phosphotyrosine-containing proteins. Science. 1990, 248: 1537-1539.
Reichman CT, Mayer BJ, Keshav S, Hanafusa H: The product of the cellular crk gene consists primarily of SH2 and SH3 regions. Cell Growth Differ. 1992, 3: 451-460.
Matsuda M, Tanaka S, Nagata S, Kojima A, Kurata T, Shibuya M: Two species of human CRK cDNA encode proteins with distinct biological activities. Mol Cell Biol. 1992, 12: 3482-3489.
Jove R, Hanafusa H: Cell transformation by the viral src oncogene. Annu Rev Cell Biol. 1987, 3: 31-56.
Wang LH, Hanafusa H: Avian sarcoma viruses. Virus Res. 1988, 9: 159-203.
Feller SM, Knudsen B, Hanafusa H: c-Abl kinase regulates the protein binding activity of c-Crk. Embo J. 1994, 13: 2341-2351.
Nada S, Yagi T, Takeda H, Tokunaga T, Nakagawa H, Ikawa Y, Okada M, Aizawa S: Constitutive activation of Src family kinases in mouse embryos that lack Csk. Cell. 1993, 73: 1125-1135.
Superti-Furga G, Fumagalli S, Koegl M, Courtneidge SA, Draetta G: Csk inhibition of c-Src activity requires both the SH2 and SH3 domains of Src. Embo J. 1993, 12: 2625-2634.
Sicheri F, Kuriyan J: Structures of Src-family tyrosine kinases. Curr Opin Struct Biol. 1997, 7: 777-785.
Rosen MK, Yamazaki T, Gish GD, Kay CM, Pawson T, Kay LE: Direct demonstration of an intramolecular SH2-phosphotyrosine interaction in the Crk protein. Nature. 1995, 374: 477-479.
Kobashigawa Y, Sakai M, Naito M, Yokochi M, Kumeta H, Makino Y, Ogura K, Tanaka S, Inagaki F: Structural basis for the transforming activity of human cancer-related signaling adaptor protein CRK. Nat Struct Mol Biol. 2007, 14: 503-510.
Greulich H, Reichman C, Hanafusa H: Delay in serum stimulation of Erk activity caused by oncogenic transformation. Oncogene. 1996, 12: 1689-1695.
Feller SM: Crk family adaptors-signalling complex formation and biological roles. Oncogene. 2001, 20: 6348-6371.
Feller SM, Knudsen B, Wong TW, Hanafusa H: Detection of SH3-binding proteins in total cell lysates with glutathione S-transferase-SH3 fusion proteins: SH3 blot assay. Methods Enzymol. 1995, 255: 369-378.
Sakai R, Iwamatsu A, Hirano N, Ogawa S, Tanaka T, Mano H, Yazaki Y, Hirai H: A novel signaling molecule, p130, forms stable complexes in vivo with v-Crk and v-Src in a tyrosine phosphorylation-dependent manner. Embo J. 1994, 13: 3748-3756.
Sattler M, Salgia R, Okuda K, Uemura N, Durstin MA, Pisick E, Xu G, Li JL, Prasad KV, Griffin JD: The proto-oncogene product p120CBL and the adaptor proteins CRKL and c-CRK link c-ABL, p190BCR/ABL and p210BCR/ABL to the phosphatidylinositol-3' kinase pathway. Oncogene. 1996, 12: 839-846.
Hempstead BL, Birge RB, Fajardo JE, Glassman R, Mahadeo D, Kraemer R, Hanafusa H: Expression of the v-crk oncogene product in PC12 cells results in rapid differentiation by both nerve growth factor- and epidermal growth factor-dependent pathways. Mol Cell Biol. 1994, 14: 1964-1971.
Ribon V, Saltiel AR: Nerve growth factor stimulates the tyrosine phosphorylation of endogenous Crk-II and augments its association with p130Cas in PC-12 cells. J Biol Chem. 1996, 271: 7375-7380.
Fukazawa T, Miyake S, Band V, Band H: Tyrosine phosphorylation of Cbl upon epidermal growth factor (EGF) stimulation and its association with EGF receptor and downstream signaling proteins. J Biol Chem. 1996, 271: 14554-14559.
Ojaniemi M, Vuori K: Epidermal growth factor modulates tyrosine phosphorylation of p130Cas. Involvement of phosphatidylinositol 3'-kinase and actin cytoskeleton. J Biol Chem. 1997, 272: 25993-25998.
Beitner-Johnson D, Blakesley VA, Shen-Orr Z, Jimenez M, Stannard B, Wang LM, Pierce J, LeRoith D: The proto-oncogene product c-Crk associates with insulin receptor substrate-1 and 4PS. Modulation by insulin growth factor-I (IGF) and enhanced IGF-I signaling. J Biol Chem. 1996, 271: 9287-9290.
Jin S, Zhai B, Qiu Z, Wu J, Lane MD, Liao K: c-Crk, a substrate of the insulin-like growth factor-1 receptor tyrosine kinase, functions as an early signal mediator in the adipocyte differentiation process. J Biol Chem. 2000, 275: 34344-34352.
Anderson D, Koch CA, Grey L, Ellis C, Moran MF, Pawson T: Binding of SH2 domains of phospholipase C gamma 1, GAP, and Src to activated growth factor receptors. Science. 1990, 250: 979-982.
Yokote K, Hellman U, Ekman S, Saito Y, Ronnstrand L, Heldin CH, Mori S: Identification of Tyr-762 in the platelet-derived growth factor alpha-receptor as the binding site for Crk proteins. Oncogene. 1998, 16: 1229-1239.
Salameh A, Galvagni F, Bardelli M, Bussolino F, Oliviero S: Direct recruitment of CRK and GRB2 to VEGFR-3 induces proliferation migration, and survival of endothelial cells through the activation of ERK AKT, and JNK pathways. Blood. 2005, 106: 3423-3431.
Garcia-Guzman M, Dolfi F, Zeh K, Vuori K: Met-induced JNK activation is mediated by the adapter protein Crk and correlates with the Gab1 – Crk signaling complex formation. Oncogene. 1999, 18: 7775-7786.
Noren NK, Foos G, Hauser CA, Pasquale EB: The EphB4 receptor suppresses breast cancer cell tumorigenicity through an Abl-Crk pathway. Nat Cell Biol. 2006, 8: 815-825.
Schraw W, Richmond A: Melanoma growth stimulatory activity signaling through the class II interleukin-8 receptor enhances the tyrosine phosphorylation of Crk-associated substrate, p130, and a 70-kilodalton protein. Biochemistry. 1995, 34: 13760-13767.
Barber DL, Mason JM, Fukazawa T, Reedquist KA, Druker BJ, Band H, D'Andrea AD: Erythropoietin and interleukin-3 activate tyrosine phosphorylation of CBL and association with CRK adaptor proteins. Blood. 1997, 89: 3166-3174.
Takahashi T, Kawahara Y, Taniguchi T, Yokoyama M: Tyrosine phosphorylation and association of p130Cas and c-Crk II by ANG II in vascular smooth muscle cells. Am J Physiol. 1998, 274: H1059-1065.
Zhang Z, Hernandez-Lagunas L, Horne WC, Baron R: Cytoskeleton-dependent tyrosine phosphorylation of the p130(Cas) family member HEF1 downstream of the G protein-coupled calcitonin receptor. Calcitonin induces the association of HEF1, paxillin, and focal adhesion kinase. J Biol Chem. 1999, 274: 25093-25098.
Oda A, Wakao H, Fujihara M, Ozaki K, Komatsu N, Tanaka S, Ikeda H, Miyajima A, Ikebuchi K: Thrombopoietin and interleukin-2 induce association of CRK with STAT5. Biochem Biophys Res Commun. 2000, 278: 299-305.
Kim JT, Joo CK: Involvement of cell-cell interactions in the rapid stimulation of Cas tyrosine phosphorylation and Src kinase activity by transforming growth factor-beta 1. J Biol Chem. 2002, 277: 31938-31948.
Zhang XF, Wang JF, Matczak E, Proper JA, Groopman JE: Janus kinase 2 is involved in stromal cell-derived factor-1alpha-induced tyrosine phosphorylation of focal adhesion proteins and migration of hematopoietic progenitor cells. Blood. 2001, 97: 3342-3348.
Chen K, Ochalski PG, Tran TS, Sahir N, Schubert M, Pramatarova A, Howell BW: Interaction between Dab1 and CrkII is promoted by Reelin signaling. J Cell Sci. 2004, 117: 4527-4536.
Sawasdikosol S, Ravichandran KS, Lee KK, Chang JH, Burakoff SJ: Crk interacts with tyrosine-phosphorylated p116 upon T cell activation. J Biol Chem. 1995, 270: 2893-2896.
Buday L, Khwaja A, Sipeki S, Farago A, Downward J: Interactions of Cbl with two adapter proteins, Grb2 and Crk, upon T cell activation. J Biol Chem. 1996, 271: 6159-6163.
Petruzzelli L, Takami M, Herrera R: Adhesion through the interaction of lymphocyte function-associated antigen-1 with intracellular adhesion molecule-1 induces tyrosine phosphorylation of p130cas and its association with c-CrkII. J Biol Chem. 1996, 271: 7796-7801.
Flinn HM, Ridley AJ: Rho stimulates tyrosine phosphorylation of focal adhesion kinase, p130 and paxillin. J Cell Sci. 1996, 109 (Pt 5): 1133-1141.
Rosado JA, Salido GM, Garcia LJ: Activation of m3 muscarinic receptors induces rapid tyrosine phosphorylation of p125(FAK), p130(cas), and paxillin in rat pancreatic acini. Arch Biochem Biophys. 2000, 377: 85-94.
Yang CC, Ogawa H, Dwinell MB, McCole DF, Eckmann L, Kagnoff MF: Chemokine receptor CCR6 transduces signals that activate p130Cas and alter cAMP-stimulated ion transport in human intestinal epithelial cells. Am J Physiol Cell Physiol. 2005, 288: C321-328.
Nojima Y, Mimura T, Morino N, Hamasaki K, Furuya H, Sakai R, Nakamoto T, Yazaki Y, Hirai H: Tyrosine phosphorylation of p130Cas in cell adhesion and transformation. Hum Cell. 1996, 9: 169-174.
Armulik A, Velling T, Johansson S: The integrin beta1 subunit transmembrane domain regulates phosphatidylinositol 3-kinase-dependent tyrosine phosphorylation of Crk-associated substrate. Mol Biol Cell. 2004, 15: 2558-2567.
Smith HW, Marra P, Marshall CJ: uPAR promotes formation of the p130Cas-Crk complex to activate Rac through DOCK180. J Cell Biol. 2008, 182: 777-790.
Eisenmann KM, McCarthy JB, Simpson MA, Keely PJ, Guan JL, Tachibana K, Lim L, Manser E, Furcht LT, Iida J: Melanoma chondroitin sulphate proteoglycan regulates cell spreading through Cdc42, Ack-1 and p130cas. Nat Cell Biol. 1999, 1: 507-513.
Okuda M, Takahashi M, Suero J, Murry CE, Traub O, Kawakatsu H, Berk BC: Shear stress stimulation of p130(cas) tyrosine phosphorylation requires calcium-dependent c-Src activation. J Biol Chem. 1999, 274: 26803-26809.
Sawada Y, Tamada M, Dubin-Thaler BJ, Cherniavskaya O, Sakai R, Tanaka S, Sheetz MP: Force sensing by mechanical extension of the Src family kinase substrate p130Cas. Cell. 2006, 127: 1015-1026.
Petit V, Boyer B, Lentz D, Turner CE, Thiery JP, Valles AM: Phosphorylation of tyrosine residues 31 and 118 on paxillin regulates cell migration through an association with CRK in NBT-II cells. J Cell Biol. 2000, 148: 957-970.
Gual P, Shigematsu S, Kanzaki M, Gremeaux T, Gonzalez T, Pessin JE, Le Marchand-Brustel Y, Tanti JF: A Crk-II/TC10 signaling pathway is required for osmotic shock-stimulated glucose transport. J Biol Chem. 2002, 277: 43980-43986.
Cho SY, Klemke RL: Extracellular-regulated kinase activation and CAS/Crk coupling regulate cell migration and suppress apoptosis during invasion of the extracellular matrix. J Cell Biol. 2000, 149: 223-236.
Gu J, Sumida Y, Sanzen N, Sekiguchi K: Laminin-10/11 and fibronectin differentially regulate integrin-dependent Rho and Rac activation via p130(Cas)-CrkII-DOCK180 pathway. J Biol Chem. 2001, 276: 27090-27097.
Oh J, Diaz T, Wei B, Chang H, Noda M, Stetler-Stevenson WG: TIMP-2 upregulates RECK expression via dephosphorylation of paxillin tyrosine residues 31 and 118. Oncogene. 2006, 25: 4230-4234.
Liu G, Li W, Gao X, Li X, Jurgensen C, Park HT, Shin NY, Yu J, He ML, Hanks SK, Wu JY, Guan KL, Rao Y: p130CAS is required for netrin signaling and commissural axon guidance. J Neurosci. 2007, 27: 957-968.
Tsuda M, Makino Y, Iwahara T, Nishihara H, Sawa H, Nagashima K, Hanafusa H, Tanaka S: Crk associates with ERM proteins and promotes cell motility toward hyaluronic acid. J Biol Chem. 2004, 279: 46843-46850.
Reddien PW, Horvitz HR: CED-2/CrkII and CED-10/Rac control phagocytosis and cell migration in Caenorhabditis elegans. Nat Cell Biol. 2000, 2: 131-136.
Albert ML, Kim JI, Birge RB: alphavbeta5 integrin recruits the CrkII-Dock180-rac1 complex for phagocytosis of apoptotic cells. Nat Cell Biol. 2000, 2: 899-905.
Stuart LM, Bell SA, Stewart CR, Silver JM, Richard J, Goss JL, Tseng AA, Zhang A, El Khoury JB, Moore KJ: CD36 signals to the actin cytoskeleton and regulates microglial migration via a p130Cas complex. J Biol Chem. 2007, 282: 27392-27401.
Li E, Stupack DG, Brown SL, Klemke R, Schlaepfer DD, Nemerow GR: Association of p130CAS with phosphatidylinositol-3-OH kinase mediates adenovirus cell entry. J Biol Chem. 2000, 275: 14729-14735.
Weidow CL, Black DS, Bliska JB, Bouton AH: CAS/Crk signalling mediates uptake of Yersinia into human epithelial cells. Cell Microbiol. 2000, 2: 549-560.
Burton EA, Plattner R, Pendergast AM: Abl tyrosine kinases are required for infection by Shigella flexneri. Embo J. 2003, 22: 5471-5479.
Sun H, Shen Y, Dokainish H, Holgado-Madruga M, Wong A, Ireton K: Host adaptor proteins Gab1 and CrkII promote InlB-dependent entry of Listeria monocytogenes. Cell Microbiol. 2005, 7: 443-457.
Suzuki M, Mimuro H, Suzuki T, Park M, Yamamoto T, Sasakawa C: Interaction of CagA with Crk plays an important role in Helicobacter pylori-induced loss of gastric epithelial cell adhesion. J Exp Med. 2005, 202: 1235-1247.
Freitas F, Jeschke M, Majstorovic I, Mueller DR, Schindler P, Voshol H, Van Oostrum J, Susa M: Fluoroaluminate stimulates phosphorylation of p130 Cas and Fak and increases attachment and spreading of preosteoblastic MC3T3-E1 cells. Bone. 2002, 30: 99-108.
Ohmori T, Yatomi Y, Okamoto H, Miura Y, Rile G, Satoh K, Ozaki Y: G(i)-mediated Cas tyrosine phosphorylation in vascular endothelial cells stimulated with sphingosine 1-phosphate: possible involvement in cell motility enhancement in cooperation with Rho-mediated pathways. J Biol Chem. 2001, 276: 5274-5280.
Endo A, Nagashima K, Kurose H, Mochizuki S, Matsuda M, Mochizuki N: Sphingosine 1-phosphate induces membrane ruffling and increases motility of human umbilical vein endothelial cells via vascular endothelial growth factor receptor and CrkII. J Biol Chem. 2002, 277: 23747-23754.
Akakura S, Singh S, Spataro M, Akakura R, Kim JI, Albert ML, Birge RB: The opsonin MFG-E8 is a ligand for the alphavbeta5 integrin and triggers DOCK180-dependent Rac1 activation for the phagocytosis of apoptotic cells. Exp Cell Res. 2004, 292: 403-416.
Hanayama R, Tanaka M, Miwa K, Shinohara A, Iwamatsu A, Nagata S: Identification of a factor that links apoptotic cells to phagocytes. Nature. 2002, 417: 182-187.
Cabodi S, Moro L, Baj G, Smeriglio M, Di Stefano P, Gippone S, Surico N, Silengo L, Turco E, Tarone G, Defilippi P: p130Cas interacts with estrogen receptor alpha and modulates non-genomic estrogen signaling in breast cancer cells. J Cell Sci. 2004, 117: 1603-1611.
Cowell LN, Graham JD, Bouton AH, Clarke CL, O'Neill GM: Tamoxifen treatment promotes phosphorylation of the adhesion molecules, p130Cas/BCAR1, FAK and Src, via an adhesion-dependent pathway. Oncogene. 2006, 25: 7597-7607.
Kirsch KH, Georgescu MM, Shishido T, Langdon WY, Birge RB, Hanafusa H: The adapter type protein CMS/CD2AP binds to the proto-oncogenic protein c-Cbl through a tyrosine phosphorylation-regulated Src homology 3 domain interaction. J Biol Chem. 2001, 276: 4957-4963.
Isakov N: A new twist to adaptor proteins contributes to regulation of lymphocyte cell signaling. Trends Immunol. 2008, 29: 388-396.
Guris DL, Fantes J, Tara D, Druker BJ, Imamoto A: Mice lacking the homologue of the human 22q11.2 gene CRKL phenocopy neurocristopathies of DiGeorge syndrome. Nat Genet. 2001, 27: 293-298.
Moon AM, Guris DL, Seo JH, Li L, Hammond J, Talbot A, Imamoto A: Crkl deficiency disrupts Fgf8 signaling in a mouse model of 22q11 deletion syndromes. Dev Cell. 2006, 10: 71-80.
Guris DL, Duester G, Papaioannou VE, Imamoto A: Dose-dependent interaction of Tbx1 and Crkl and locally aberrant RA signaling in a model of del22q11 syndrome. Dev Cell. 2006, 10: 81-92.
Park TJ, Boyd K, Curran T: Cardiovascular and craniofacial defects in Crk-null mice. Mol Cell Biol. 2006, 26: 6272-6282.
Imaizumi T, Araki K, Miura K, Araki M, Suzuki M, Terasaki H, Yamamura K: Mutant mice lacking Crk-II caused by the gene trap insertional mutagenesis: Crk-II is not essential for embryonic development. Biochem Biophys Res Commun. 1999, 266: 569-574.
D'Arcangelo G, Homayouni R, Keshvara L, Rice DS, Sheldon M, Curran T: Reelin is a ligand for lipoprotein receptors. Neuron. 1999, 24: 471-479.
Huang Y, Magdaleno S, Hopkins R, Slaughter C, Curran T, Keshvara L: Tyrosine phosphorylated Disabled 1 recruits Crk family adapter proteins. Biochem Biophys Res Commun. 2004, 318: 204-212.
Yip YP, Kronstadt-O'Brien P, Capriotti C, Cooper JA, Yip JW: Migration of sympathetic preganglionic neurons in the spinal cord is regulated by Reelin-dependent Dab1 tyrosine phosphorylation and CrkL. J Comp Neurol. 2007, 502: 635-643.
Feng L, Cooper JA: Dual Functions of Dab1 during Brain Development. Mol Cell Biol. 2009, 29: 324-332.
Matsuki T, Pramatarova A, Howell BW: Reduction of Crk and CrkL expression blocks reelin-induced dendritogenesis. J Cell Sci. 2008, 121: 1869-1875.
Park TJ, Curran T: Crk and Crk-like play essential overlapping roles downstream of disabled-1 in the Reelin pathway. J Neurosci. 2008, 28: 13551-13562.
Sakai R, Nakamoto T, Ozawa K, Aizawa S, Hirai H: Characterization of the kinase activity essential for tyrosine phosphorylation of p130Cas in fibroblasts. Oncogene. 1997, 14: 1419-1426.
Akagi T, Shishido T, Murata K, Hanafusa H: v-Crk activates the phosphoinositide 3-kinase/AKT pathway in transformation. Proc Natl Acad Sci USA. 2000, 97: 7290-7295.
Iwahara T, Akagi T, Fujitsuka Y, Hanafusa H: CrkII regulates focal adhesion kinase activation by making a complex with Crk-associated substrate, p130Cas. Proc Natl Acad Sci USA. 2004, 101: 17693-17698.
Yeo MG, Sung BH, Oh HJ, Park ZY, Marcantonio EE, Song WK: Focal adhesion targeting of v-Crk is essential for FAK phosphorylation and cell migration in mouse embryo fibroblasts deficient src family kinases or p130CAS. J Cell Physiol. 2008, 214: 604-613.
Defilippi P, Di Stefano P, Cabodi S: p130Cas: a versatile scaffold in signaling networks. Trends Cell Biol. 2006, 16: 257-263.
Birge RB, Fajardo JE, Mayer BJ, Hanafusa H: Tyrosine-phosphorylated epidermal growth factor receptor and cellular p130 provide high affinity binding substrates to analyze Crk-phosphotyrosine-dependent interactions in vitro. J Biol Chem. 1992, 267: 10588-10595.
Auvinen M, Paasinen-Sohns A, Hirai H, Andersson LC, Holtta E: Ornithine decarboxylase- and ras-induced cell transformations: reversal by protein tyrosine kinase inhibitors and role of pp130CAS. Mol Cell Biol. 1995, 15: 6513-6525.
Honda H, Oda H, Nakamoto T, Honda Z, Sakai R, Suzuki T, Saito T, Nakamura K, Nakao K, Ishikawa T, Katsuki M, Yazaki Y, Hirai H: Cardiovascular anomaly, impaired actin bundling and resistance to Src-induced transformation in mice lacking p130Cas. Nat Genet. 1998, 19: 361-365.
Cabodi S, Tinnirello A, Di Stefano P, Bisaro B, Ambrosino E, Castellano I, Sapino A, Arisio R, Cavallo F, Forni G, Glukhova M, Silengo L, Altruda F, Turco E, Tarone G, Defilippi P: p130Cas as a new regulator of mammary epithelial cell proliferation survival, and HER2-neu oncogene-dependent breast tumorigenesis. Cancer Res. 2006, 66: 4672-4680.
Schrecengost RS, Riggins RB, Thomas KS, Guerrero MS, Bouton AH: Breast cancer antiestrogen resistance-3 expression regulates breast cancer cell migration through promotion of p130Cas membrane localization and membrane ruffling. Cancer Res. 2007, 67: 6174-6182.
Ta HQ, Thomas KS, Schrecengost RS, Bouton AH: A novel association between p130Cas and resistance to the chemotherapeutic drug adriamycin in human breast cancer cells. Cancer Res. 2008, 68: 8796-8804.
Barkan D, Kleinman H, Simmons JL, Asmussen H, Kamaraju AK, Hoenorhoff MJ, Liu ZY, Costes SV, Cho EH, Lockett S, Khanna C, Chambers AF, Green JE: Inhibition of metastatic outgrowth from single dormant tumor cells by targeting the cytoskeleton. Cancer Res. 2008, 68: 6241-6250.
Kirsch K, Kensinger M, Hanafusa H, August A: A p130Cas tyrosine phosphorylated substrate domain decoy disrupts v-crk signaling. BMC Cell Biol. 2002, 3: 18-
Huang Z, Yazdani U, Thompson-Peer KL, Kolodkin AL, Terman JR: Crk-associated substrate (Cas) signaling protein functions with integrins to specify axon guidance during development. Development. 2007, 134: 2337-2347.
Nievers MG, Birge RB, Greulich H, Verkleij AJ, Hanafusa H, van Bergen en Henegouwen PM: v-Crk-induced cell transformation: changes in focal adhesion composition and signaling. J Cell Sci. 1997, 110 (Pt 3): 389-399.
Yeo MG, Sung BH, Oh HJ, Park ZY, Marcantonio EE, Song WK: Focal adhesion targeting of v-Crk is essential for FAK phosphorylation and cell migration in mouse embryo fibroblasts deficient src family kinases or p130CAS. J Cell Physiol. 2008, 214 (3): 604-13.
Pellicena P, Miller WT: Processive phosphorylation of p130Cas by Src depends on SH3-polyproline interactions. J Biol Chem. 2001, 276: 28190-28196.
Patwardhan P, Shen Y, Goldberg GS, Miller WT: Individual Cas phosphorylation sites are dispensable for processive phosphorylation by Src and anchorage-independent cell growth. J Biol Chem. 2006, 281: 20689-20697.
Cary LA, Han DC, Polte TR, Hanks SK, Guan JL: Identification of p130Cas as a mediator of focal adhesion kinase-promoted cell migration. J Cell Biol. 1998, 140: 211-221.
Polte TR, Hanks SK: Interaction between focal adhesion kinase and Crk-associated tyrosine kinase substrate p130Cas. Proc Natl Acad Sci USA. 1995, 92: 10678-10682.
Astier A, Avraham H, Manie SN, Groopman J, Canty T, Avraham S, Freedman AS: The related adhesion focal tyrosine kinase is tyrosine-phosphorylated after beta1-integrin stimulation in B cells and binds to p130cas. J Biol Chem. 1997, 272: 228-232.
Chodniewicz D, Klemke RL: Regulation of integrin-mediated cellular responses through assembly of a CAS/Crk scaffold. Biochim Biophys Acta. 2004, 1692: 63-76.
Sharma A, Mayer BJ: Phosphorylation of p130Cas initiates Rac activation and membrane ruffling. BMC Cell Biol. 2008, 9: 50-
Tazaki T, Miyazaki K, Hiyama E, Nakamoto T, Sakai R, Yamasaki N, Honda Z, Noda M, Miyasaka N, Sueda T, Honda H: Functional analysis of Src homology 3-encoding exon (exon 2) of p130Cas in primary fibroblasts derived from exon 2-specific knockout mice. Genes Cells. 2008, 13: 145-157.
Birge RB, Fajardo JE, Reichman C, Shoelson SE, Songyang Z, Cantley LC, Hanafusa H: Identification and characterization of a high-affinity interaction between v-Crk and tyrosine-phosphorylated paxillin in CT10-transformed fibroblasts. Mol Cell Biol. 1993, 13: 4648-4656.
Watanabe T, Tsuda M, Makino Y, Ichihara S, Sawa H, Minami A, Mochizuki N, Nagashima K, Tanaka S: Adaptor molecule Crk is required for sustained phosphorylation of Grb2-associated binder 1 and hepatocyte growth factor-induced cell motility of human synovial sarcoma cell lines. Mol Cancer Res. 2006, 4: 499-510.
Lamorte L, Kamikura DM, Park M: A switch from p130Cas/Crk to Gab1/Crk signaling correlates with anchorage independent growth and JNK activation in cells transformed by the Met receptor oncoprotein. Oncogene. 2000, 19: 5973-5981.
Tanaka S, Morishita T, Hashimoto Y, Hattori S, Nakamura S, Shibuya M, Matuoka K, Takenawa T, Kurata T, Nagashima K, et al.: C3G, a guanine nucleotide-releasing protein expressed ubiquitously, binds to the Src homology 3 domains of CRK and GRB2/ASH proteins. Proc Natl Acad Sci USA. 1994, 91: 3443-3447.
Knudsen BS, Feller SM, Hanafusa H: Four proline-rich sequences of the guanine-nucleotide exchange factor C3G bind with unique specificity to the first Src homology 3 domain of Crk. J Biol Chem. 1994, 269: 32781-32787.
Gotoh T, Hattori S, Nakamura S, Kitayama H, Noda M, Takai Y, Kaibuchi K, Matsui H, Hatase O, Takahashi H, et al.: Identification of Rap1 as a target for the Crk SH3 domain-binding guanine nucleotide-releasing factor C3G. Mol Cell Biol. 1995, 15: 6746-6753.
Altschuler DL, Ribeiro-Neto F: Mitogenic and oncogenic properties of the small G protein Rap1b. Proc Natl Acad Sci USA. 1998, 95: 7475-7479.
Yoshida Y, Kawata M, Miura Y, Musha T, Sasaki T, Kikuchi A, Takai Y: Microinjection of smg/rap1/Krev-1 p21 into Swiss 3T3 cells induces DNA synthesis and morphological changes. Mol Cell Biol. 1992, 12: 3407-3414.
Ishida D, Kometani K, Yang H, Kakugawa K, Masuda K, Iwai K, Suzuki M, Itohara S, Nakahata T, Hiai H, Kawamoto H, Hattori M, Minato N: Myeloproliferative stem cell disorders by deregulated Rap1 activation in SPA-1-deficient mice. Cancer Cell. 2003, 4: 55-65.
Minato N, Hattori M: Spa-1 (Sipa1) and Rap signaling in leukemia and cancer metastasis. Cancer Sci. 2009, 100 (1): 17-23. Epub 2008 Nov 24
Wang SF, Aoki M, Nakashima Y, Shinozuka Y, Tanaka H, Taniwaki M, Hattori M, Minato N: Development of Notch-dependent T-cell leukemia by deregulated Rap1 signaling. Blood. 2008, 111: 2878-2886.
Tanaka S, Ouchi T, Hanafusa H: Downstream of Crk adaptor signaling pathway: activation of Jun kinase by v-Crk through the guanine nucleotide exchange protein C3G. Proc Natl Acad Sci USA. 1997, 94: 2356-2361.
Tanaka S, Hanafusa H: Guanine-nucleotide exchange protein C3G activates JNK1 by a ras-independent mechanism. JNK1 activation inhibited by kinase negative forms of MLK3 and DLK mixed lineage kinases. J Biol Chem. 1998, 273: 1281-1284.
De Falco V, Castellone MD, De Vita G, Cirafici AM, Hershman JM, Guerrero C, Fusco A, Melillo RM, Santoro M: RET/papillary thyroid carcinoma oncogenic signaling through the Rap1 small GTPase. Cancer Res. 2007, 67: 381-390.
Martin-Encabo S, Santos E, Guerrero C: C3G mediated suppression of malignant transformation involves activation of PP2A phosphatases at the subcortical actin cytoskeleton. Exp Cell Res. 2007, 313: 3881-3891.
Gutierrez-Berzal J, Castellano E, Martin-Encabo S, Gutierrez-Cianca N, Hernandez JM, Santos E, Guerrero C: Characterization of p87C3G, a novel, truncated C3G isoform that is overexpressed in chronic myeloid leukemia and interacts with Bcr-Abl. Exp Cell Res. 2006, 312: 938-948.
Bos JL: Linking Rap to cell adhesion. Curr Opin Cell Biol. 2005, 17: 123-128.
Katagiri K, Hattori M, Minato N, Irie S, Takatsu K, Kinashi T: Rap1 is a potent activation signal for leukocyte function-associated antigen 1 distinct from protein kinase C and phosphatidylinositol-3-OH kinase. Mol Cell Biol. 2000, 20: 1956-1969.
Lafuente EM, van Puijenbroek AA, Krause M, Carman CV, Freeman GJ, Berezovskaya A, Constantine E, Springer TA, Gertler FB, Boussiotis VA: RIAM, an Ena/VASP and Profilin ligand, interacts with Rap1-GTP and mediates Rap1-induced adhesion. Dev Cell. 2004, 7: 585-595.
Hogan C, Serpente N, Cogram P, Hosking CR, Bialucha CU, Feller SM, Braga VM, Birchmeier W, Fujita Y: Rap1 regulates the formation of E-cadherin-based cell-cell contacts. Mol Cell Biol. 2004, 24: 6690-6700.
Ichiba T, Hashimoto Y, Nakaya M, Kuraishi Y, Tanaka S, Kurata T, Mochizuki N, Matsuda M: Activation of C3G guanine nucleotide exchange factor for Rap1 by phosphorylation of tyrosine 504. J Biol Chem. 1999, 274: 14376-14381.
Radha V, Rajanna A, Swarup G: Phosphorylated guanine nucleotide exchange factor C3G, induced by pervanadate and Src family kinases localizes to the Golgi and subcortical actin cytoskeleton. BMC Cell Biol. 2004, 5: 31-
York RD, Yao H, Dillon T, Ellig CL, Eckert SP, McCleskey EW, Stork PJ: Rap1 mediates sustained MAP kinase activation induced by nerve growth factor. Nature. 1998, 392: 622-626.
Simon MA, Dodson GS, Rubin GM: An SH3-SH2-SH3 protein is required for p21Ras1 activation and binds to sevenless and Sos proteins in vitro. Cell. 1993, 73: 169-177.
Chardin P, Camonis JH, Gale NW, van Aelst L, Schlessinger J, Wigler MH, Bar-Sagi D: Human Sos1: a guanine nucleotide exchange factor for Ras that binds to GRB2. Science. 1993, 260: 1338-1343.
Innocenti M, Tenca P, Frittoli E, Faretta M, Tocchetti A, Di Fiore PP, Scita G: Mechanisms through which Sos-1 coordinates the activation of Ras and Rac. J Cell Biol. 2002, 156: 125-136.
Pierpont EI, Pierpont ME, Mendelsohn NJ, Roberts AE, Tworog-Dube E, Seidenberg MS: Genotype differences in cognitive functioning in Noonan syndrome. Genes Brain Behav. 2009, 8 (3): 275-82. Epub 2008 Dec 11
Roberts AE, Araki T, Swanson KD, Montgomery KT, Schiripo TA, Joshi VA, Li L, Yassin Y, Tamburino AM, Neel BG, Kucherlapati RS: Germline gain-of-function mutations in SOS1 cause Noonan syndrome. Nat Genet. 2007, 39: 70-74.
Hasegawa H, Kiyokawa E, Tanaka S, Nagashima K, Gotoh N, Shibuya M, Kurata T, Matsuda M: DOCK180, a major CRK-binding protein, alters cell morphology upon translocation to the cell membrane. Mol Cell Biol. 1996, 16: 1770-1776.
Matsuda M, Ota S, Tanimura R, Nakamura H, Matuoka K, Takenawa T, Nagashima K, Kurata T: Interaction between the amino-terminal SH3 domain of CRK and its natural target proteins. J Biol Chem. 1996, 271: 14468-14472.
Brugnera E, Haney L, Grimsley C, Lu M, Walk SF, Tosello-Trampont AC, Macara IG, Madhani H, Fink GR, Ravichandran KS: Unconventional Rac-GEF activity is mediated through the Dock180-ELMO complex. Nat Cell Biol. 2002, 4: 574-582.
Lu M, Kinchen JM, Rossman KL, Grimsley C, deBakker C, Brugnera E, Tosello-Trampont AC, Haney LB, Klingele D, Sondek J, Hengartner MO, Ravichandran KS: PH domain of ELMO functions in trans to regulate Rac activation via Dock180. Nat Struct Mol Biol. 2004, 11: 756-762.
Park D, Tosello-Trampont AC, Elliott MR, Lu M, Haney LB, Ma Z, Klibanov AL, Mandell JW, Ravichandran KS: BAI1 is an engulfment receptor for apoptotic cells upstream of the ELMO/Dock180/Rac module. Nature. 2007, 450: 430-434.
deBakker CD, Haney LB, Kinchen JM, Grimsley C, Lu M, Klingele D, Hsu PK, Chou BK, Cheng LC, Blangy A, Sondek J, Hengartner MO, Wu YC, Ravichandran KS: Phagocytosis of apoptotic cells is regulated by a UNC-73/TRIO-MIG-2/RhoG signaling module and armadillo repeats of CED-12/ELMO. Curr Biol. 2004, 14: 2208-2216.
Makino Y, Tsuda M, Ichihara S, Watanabe T, Sakai M, Sawa H, Nagashima K, Hatakeyama S, Tanaka S: Elmo1 inhibits ubiquitylation of Dock180. J Cell Sci. 2006, 119: 923-932.
Kiyokawa E, Hashimoto Y, Kurata T, Sugimura H, Matsuda M: Evidence that DOCK180 up-regulates signals from the CrkII-p130(Cas) complex. J Biol Chem. 1998, 273: 24479-24484.
Tosello-Trampont AC, Kinchen JM, Brugnera E, Haney LB, Hengartner MO, Ravichandran KS: Identification of two signaling submodules within the CrkII/ELMO/Dock180 pathway regulating engulfment of apoptotic cells. Cell Death Differ. 2007, 14: 963-972.
Akakura S, Kar B, Singh S, Cho L, Tibrewal N, Sanokawa-Akakura R, Reichman C, Ravichandran KS, Birge RB: C-terminal SH3 domain of CrkII regulates the assembly and function of the DOCK180/ELMO Rac-GEF. J Cell Physiol. 2005, 204: 344-351.
Cote JF, Vuori K: Identification of an evolutionarily conserved superfamily of DOCK180-related proteins with guanine nucleotide exchange activity. J Cell Sci. 2002, 115: 4901-4913.
Cote JF, Vuori K: GEF what? Dock180 and related proteins help Rac to polarize cells in new ways. Trends Cell Biol. 2007, 17: 383-393.
Meller N, Merlot S, Guda C: CZH proteins: a new family of Rho-GEFs. J Cell Sci. 2005, 118: 4937-4946.
Sanders MA, Ampasala D, Basson MD: DOCK5 and DOCK1 Regulate Caco-2 Intestinal Epithelial Cell Spreading and Migration on Collagen IV. J Biol Chem. 2009, 284: 27-35.
Nishihara H, Maeda M, Oda A, Tsuda M, Sawa H, Nagashima K, Tanaka S: DOCK2 associates with CrkL and regulates Rac1 in human leukemia cell lines. Blood. 2002, 100: 3968-3974.
Laurin M, Fradet N, Blangy A, Hall A, Vuori K, Cote JF: The atypical Rac activator Dock180 (Dock1) regulates myoblast fusion in vivo. Proc Natl Acad Sci USA. 2008, 105: 15446-15451.
Girardin SE, Yaniv M: A direct interaction between JNK1 and CrkII is critical for Rac1-induced JNK activation. Embo J. 2001, 20: 3437-3446.
Oktay M, Wary KK, Dans M, Birge RB, Giancotti FG: Integrin-mediated activation of focal adhesion kinase is required for signaling to Jun NH2-terminal kinase and progression through the G1 phase of the cell cycle. J Cell Biol. 1999, 145: 1461-1469.
Ishimaru S, Ueda R, Hinohara Y, Ohtani M, Hanafusa H: PVR plays a critical role via JNK activation in thorax closure during Drosophila metamorphosis. Embo J. 2004, 23: 3984-3994.
Ren R, Ye ZS, Baltimore D: Abl protein-tyrosine kinase selects the Crk adapter as a substrate using SH3-binding sites. Genes Dev. 1994, 8: 783-795.
Antoku S, Saksela K, Rivera GM, Mayer BJ: A crucial role in cell spreading for the interaction of Abl PxxP motifs with Crk and Nck adaptors. J Cell Sci. 2008, 121: 3071-3082.
Lewis JM, Schwartz MA: Integrins regulate the association and phosphorylation of paxillin by c-Abl. J Biol Chem. 1998, 273: 14225-14230.
Taagepera S, McDonald D, Loeb JE, Whitaker LL, McElroy AK, Wang JY, Hope TJ: Nuclear-cytoplasmic shuttling of C-ABL tyrosine kinase. Proc Natl Acad Sci USA. 1998, 95: 7457-7462.
Zandy NL, Playford M, Pendergast AM: Abl tyrosine kinases regulate cell-cell adhesion through Rho GTPases. Proc Natl Acad Sci USA. 2007, 104: 17686-17691.
Sini P, Cannas A, Koleske AJ, Di Fiore PP, Scita G: Abl-dependent tyrosine phosphorylation of Sos-1 mediates growth-factor-induced Rac activation. Nat Cell Biol. 2004, 6: 268-274.
Shishido T, Akagi T, Chalmers A, Maeda M, Terada T, Georgescu MM, Hanafusa H: Crk family adaptor proteins trans-activate c-Abl kinase. Genes Cells. 2001, 6: 431-440.
Reichman C, Singh K, Liu Y, Singh S, Li H, Fajardo JE, Fiser A, Birge RB: Transactivation of Abl by the Crk II adapter protein requires a PNAY sequence in the Crk C-terminal SH3 domain. Oncogene. 2005, 24: 8187-8199.
Woodring PJ, Hunter T, Wang JY: Mitotic phosphorylation rescues Abl from F-actin-mediated inhibition. J Biol Chem. 2005, 280: 10318-10325.
Anafi M, Rosen MK, Gish GD, Kay LE, Pawson T: A potential SH3 domain-binding site in the Crk SH2 domain. J Biol Chem. 1996, 271: 21365-21374.
Donaldson LW, Gish G, Pawson T, Kay LE, Forman-Kay JD: Structure of a regulatory complex involving the Abl SH3 domain, the Crk SH2 domain, and a Crk-derived phosphopeptide. Proc Natl Acad Sci USA. 2002, 99: 14053-14058.
Huang X, Wu D, Jin H, Stupack D, Wang JY: Induction of cell retraction by the combined actions of Abl-CrkII and Rho-ROCK1 signaling. J Cell Biol. 2008, 183: 711-723.
Cipres A, Abassi YA, Vuori K: Abl functions as a negative regulator of Met-induced cell motility via phosphorylation of the adapter protein CrkII. Cell Signal. 2007, 19: 1662-1670.
Peterson ME, Long EO: Inhibitory receptor signaling via tyrosine phosphorylation of the adaptor Crk. Immunity. 2008, 29: 578-588.
Abassi YA, Vuori K: Tyrosine 221 in Crk regulates adhesion-dependent membrane localization of Crk and Rac and activation of Rac signaling. Embo J. 2002, 21: 4571-4582.
Escalante M, Courtney J, Chin WG, Teng KK, Kim JI, Fajardo JE, Mayer BJ, Hempstead BL, Birge RB: Phosphorylation of c-Crk II on the negative regulatory Tyr222 mediates nerve growth factor-induced cell spreading and morphogenesis. J Biol Chem. 2000, 275: 24787-24797.
Wu X, Knudsen B, Feller SM, Zheng J, Sali A, Cowburn D, Hanafusa H, Kuriyan J: Structural basis for the specific interaction of lysine-containing proline-rich peptides with the N-terminal SH3 domain of c-Crk. Structure. 1995, 3: 215-226.
Harkiolaki M, Gilbert RJ, Jones EY, Feller SM: The C-terminal SH3 domain of CRKL as a dynamic dimerization module transiently exposing a nuclear export signal. Structure. 2006, 14: 1741-1753.
Muralidharan V, Dutta K, Cho J, Vila-Perello M, Raleigh DP, Cowburn D, Muir TW: Solution structure and folding characteristics of the C-terminal SH3 domain of c-Crk-II. Biochemistry. 2006, 45: 8874-8884.
Cowburn D: Moving parts: how the adaptor protein CRK is regulated, and regulates. Nat Struct Mol Biol. 2007, 14: 465-466.
Sarkar P, Reichman C, Saleh T, Birge RB, Kalodimos CG: Proline cis-trans isomerization controls autoinhibition of a signaling protein. Mol Cell. 2007, 25: 413-426.
Ota S, Kizaka-Kondoh S, Hashimoto Y, Nishihara H, Nagashima K, Kurata T, Okayama H, Matsuda M: Constitutive association of EGF receptor with the CrkII-23 mutant that inhibits transformation of NRK cells by EGF and TGF-beta. Cell Signal. 1998, 10: 283-290.
Zvara A, Fajardo JE, Escalante M, Cotton G, Muir T, Kirsch KH, Birge RB: Activation of the focal adhesion kinase signaling pathway by structural alterations in the carboxyl-terminal region of c-Crk II. Oncogene. 2001, 20: 951-961.
Smith JJ, Richardson DA, Kopf J, Yoshida M, Hollingsworth RE, Kornbluth S: Apoptotic regulation by the Crk adapter protein mediated by interactions with Wee1 and Crm1/exportin. Mol Cell Biol. 2002, 22: 1412-1423.
Kar B, Reichman CT, Singh S, O'Connor JP, Birge RB: Proapoptotic function of the nuclear Crk II adaptor protein. Biochemistry. 2007, 46: 10828-10840.
Hengartner MO, Horvitz HR: The ins and outs of programmed cell death during C. elegans development. Philos Trans R Soc Lond B Biol Sci. 1994, 345: 243-246.
Metzstein MM, Stanfield GM, Horvitz HR: Genetics of programmed cell death in C. elegans: past, present and future. Trends Genet. 1998, 14: 410-416.
Hengartner MO: Apoptosis: corralling the corpses. Cell. 2001, 104: 325-328.
Bruce-Staskal PJ, Weidow CL, Gibson JJ, Bouton AH: Cas, Fak and Pyk2 function in diverse signaling cascades to promote Yersinia uptake. J Cell Sci. 2002, 115: 2689-2700.
Deng Q, Sun J, Barbieri JT: Uncoupling Crk signal transduction by Pseudomonas exoenzyme T. J Biol Chem. 2005, 280: 35953-35960.
Pielage JF, Powell KR, Kalman D, Engel JN: RNAi screen reveals an Abl kinase-dependent host cell pathway involved in Pseudomonas aeruginosa internalization. PLoS Pathog. 2008, 4: e1000031-
Heikkinen LS, Kazlauskas A, Melen K, Wagner R, Ziegler T, Julkunen I, Saksela K: Avian and 1918 Spanish influenza a virus NS1 proteins bind to Crk/CrkL Src homology 3 domains to activate host cell signaling. J Biol Chem. 2008, 283: 5719-5727.
Miller CT, Chen G, Gharib TG, Wang H, Thomas DG, Misek DE, Giordano TJ, Yee J, Orringer MB, Hanash SM, Beer DG: Increased C-CRK proto-oncogene expression is associated with an aggressive phenotype in lung adenocarcinomas. Oncogene. 2003, 22: 7950-7957.
Takino T, Nakada M, Miyamori H, Yamashita J, Yamada KM, Sato H: CrkI adapter protein modulates cell migration and invasion in glioblastoma. Cancer Res. 2003, 63: 2335-2337.
Linghu H, Tsuda M, Makino Y, Sakai M, Watanabe T, Ichihara S, Sawa H, Nagashima K, Mochizuki N, Tanaka S: Involvement of adaptor protein Crk in malignant feature of human ovarian cancer cell line MCAS. Oncogene. 2006, 25: 3547-3556.
Wang L, Tabu K, Kimura T, Tsuda M, Linghu H, Tanino M, Kaneko S, Nishihara H, Tanaka S: Signaling adaptor protein Crk is indispensable for malignant feature of glioblastoma cell line KMG4. Biochem Biophys Res Commun. 2007, 362: 976-981.
Nishihara H, Tanaka S, Tsuda M, Oikawa S, Maeda M, Shimizu M, Shinomiya H, Tanigami A, Sawa H, Nagashima K: Molecular and immunohistochemical analysis of signaling adaptor protein Crk in human cancers. Cancer Lett. 2002, 180: 55-61.
Crawford M, Brawner E, Batte K, Yu L, Hunter MG, Otterson GA, Nuovo G, Marsh CB, Nana-Sinkam SP: MicroRNA-126 inhibits invasion in non-small cell lung carcinoma cell lines. Biochem Biophys Res Commun. 2008, 373: 607-612.
Jarzynka MJ, Hu B, Hui KM, Bar-Joseph I, Gu W, Hirose T, Haney LB, Ravichandran KS, Nishikawa R, Cheng SY: ELMO1 and Dock180, a bipartite Rac1 guanine nucleotide exchange factor, promote human glioma cell invasion. Cancer Res. 2007, 67: 7203-7211.
Hirata T, Nagai H, Koizumi K, Okino K, Harada A, Onda M, Nagahata T, Mikami I, Hirai K, Haraguchi S, Jin E, Kawanami O, Shimizu K, Emi M: Amplification, up-regulation and over-expression of C3G (CRK SH3 domain-binding guanine nucleotide-releasing factor) in non-small cell lung cancers. J Hum Genet. 2004, 49: 290-295.
Fukuyama T, Ogita H, Kawakatsu T, Fukuhara T, Yamada T, Sato T, Shimizu K, Nakamura T, Matsuda M, Takai Y: Involvement of the c-Src-Crk-C3G-Rap1 signaling in the nectin-induced activation of Cdc42 and formation of adherens junctions. J Biol Chem. 2005, 280: 815-825.
Feller SM, Posern G, Voss J, Kardinal C, Sakkab D, Zheng J, Knudsen BS: Physiological signals and oncogenesis mediated through Crk family adapter proteins. J Cell Physiol. 1998, 177: 535-552.
Oda T, Heaney C, Hagopian JR, Okuda K, Griffin JD, Druker BJ: Crkl is the major tyrosine-phosphorylated protein in neutrophils from patients with chronic myelogenous leukemia. J Biol Chem. 1994, 269: 22925-22928.
Salgia R, Uemura N, Okuda K, Li JL, Pisick E, Sattler M, de Jong R, Druker B, Heisterkamp N, Chen LB, et al.: CRKL links p210BCR/ABL with paxillin in chronic myelogenous leukemia cells. J Biol Chem. 1995, 270: 29145-29150.
Ozaki K, Oda A, Wakao H, Rhodes J, Druker BJ, Ishida A, Wakui M, Okamoto S, Morita K, Handa M, et al.: Thrombopoietin induces association of Crkl with STAT5 but not STAT3 in human platelets. Blood. 1998, 92: 4652-4662.
Rhodes J, York RD, Tara D, Tajinda K, Druker BJ: CrkL functions as a nuclear adaptor and transcriptional activator in Bcr-Abl-expressing cells. Exp Hematol. 2000, 28: 305-310.
Lekmine F, Sassano A, Uddin S, Majchrzak B, Miura O, Druker BJ, Fish EN, Imamoto A, Platanias LC: The CrkL adapter protein is required for type I interferon-dependent gene transcription and activation of the small G-protein Rap1. Biochem Biophys Res Commun. 2002, 291: 744-750.
Uddin S, Lekmine F, Sassano A, Rui H, Fish EN, Platanias LC: Role of Stat5 in type I interferon-signaling and transcriptional regulation. Biochem Biophys Res Commun. 2003, 308: 325-330.
Smith JJ, Evans EK, Murakami M, Moyer MB, Moseley MA, Woude GV, Kornbluth S: Wee1-regulated apoptosis mediated by the crk adaptor protein in Xenopus egg extracts. J Cell Biol. 2000, 151: 1391-1400.
We would like to thank Sriram Ganapathy and Stephan Feller for critical comments on the manuscript. The authors would like to dedicate this article for our mentor and colleague, Saburo Hanafusa (1929–2009).
The authors declare that they have no competing interests.
RB wrote the background and significance and the sections on the biological significance of Crk. CK and FI wrote parts of the sections on Crk structure. ST wrote the sections on Crk and Cancers. All authors read and approved the final manuscript.
Electronic supplementary material
Additional file 1: A rotational view of the Crk II structure is shown in the supplemental data. The organization of the SH2 and SH3 domains are indicated in order to illustrate negative regulation. (GIF 3 MB)
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Birge, R.B., Kalodimos, C., Inagaki, F. et al. Crk and CrkL adaptor proteins: networks for physiological and pathological signaling. Cell Commun Signal 7, 13 (2009). https://doi.org/10.1186/1478-811X-7-13
- Focal Adhesion Kinase
- Tyrosine Phosphorylation
- Chronic Myelogenous Leukemia
- Noonan Syndrome
- Tyrosine Kinase Oncogene