Environmental control of invasiveness and metastatic dissemination of tumor cells: the role of tumor cell-host cell interactions
© Calorini and Bianchini; licensee BioMed Central Ltd. 2010
Received: 17 May 2010
Accepted: 7 September 2010
Published: 7 September 2010
Recent advances in tumor biology led to the realization that, in order to understand the mechanisms involved in proliferation and invasion of tumor cells, an analysis of the complex interactions that tumor cells establish with host cells of tumor microenvironment is required. The bidirectional interactions between tumor cells and components of tumor microenvironment, in particular endothelial cells, cells of monocyte/macrophage lineage and fibroblasts/myofibroblasts, play a critical role in most of the events that characterize tumor progression and metastasis. Interactions between these "reactive" normal cells and the genetically altered tumor cells, by either cell-to-cell contacts or soluble mediators, control the most aspects of tumor formation and progression. This review addresses some of the experimental evidences documenting that tumor cells may influence host cells of their own microenvironment by triggering changes that facilitate their local as well as distant dissemination. Therefore, it focuses on macrophages and fibroblasts that, upon stimulation by tumor cells, change their state towards a tumor-promoting-like phenotype.
Cancer as a non-homogeneous mass
Most primary tumors can be treated successfully by surgery alone or in combination with chemotherapy, immunotherapy or radiotherapy. However, the treatment of disseminating tumors, once they have spread to secondary sites, is a much more difficult task. Effective treatment of multiple metastatic lesions by surgery or radiotherapy is usually impossible due to their distribution in vital organs. Tumor cell invasiveness and metastasis occur by a complex series of events in which malignant cells invade host tissues, penetrate into body cavities, lymphatic and/or blood circulatory systems. Subsequently they disseminate to distant sites where they invade into new surrounding tissues and proliferate to form secondary tumors [1–3]. Metastatic diffusion of cancer cells remains the most important clinical problem, and malignancy is represented by the ability of tumor cells to invade adjacent host tissue at the primary site and then to diffuse and colonize secondary organs.
Epidemiological and experimental evidences suggest that a wide variability exists in the metastatic spread of different human malignancies, but even cancers of the same histological type often produce quite diverse disease progression and survival outcomes for individual patients . This suggests that even in the same histological class of cancer, the expected incidence and localization of metastatic lesions is not completely certain [4, 5].
The capacity of cancers to evolve and change during their development has been termed "tumor progression" by L. Foulds (1954) . The biological characteristics that define tumor progression have been extensively described, although the underlying mechanisms are still not completely defined. Tumor cells, during their sometimes decade-long development, accumulate increasingly genetic alterations, which are typically generated by random mutational events, finally allowing them to assume all the characteristics of an invasive and metastatic cancer. In concert with this "genetic instability", a key role in favouring genetic/epigenetic changes in tumor cells is played by local as well as systemic host factors [7–9]. It has been demonstrated that metastatic dissemination can be influenced by diet [10–13], the neuro-endocrine state  and inflammatory-reparative processes [15–17]. Among the local factors, particular attention has been devoted to the interactions that tumor cells establish with various noncancerous types of cells that reside in or are attracted to the tumor microenvironment. In particular, the interaction of tumor cells with platelets , lymphocytes , polymorphonuclear cells [20, 21], fibroblasts [22–25] and monocytes/macrophages was proved to be relevant to tumor progression [26, 27]. Under certain conditions, stromal cells may inhibit tumor growth, but in other cases, they can stimulate the growth and invasiveness of tumor cells. Therefore, host cells, mainly in proximity to tumor cells, may markedly alter tumor growth and invasiveness. A bidirectional interaction between tumor cells and host cells, is now recognized as crucial for the decision of whether tumor cells progress toward metastatic dissemination or whether they remain dormant [28, 29]. Numerous bioactive agents such as proteins of the extracellular matrix, growth factors, cytokines, chemokines and other molecules secreted by host cells contribute to the evolution of tumor cells, including the generation of a metastatic phenotype. It is hence important to recognize that tumors, like normal tissues, are dependent on the formation of a reactive stroma. Nicolson has postulated that the acquisition of a malignant phenotype in tumor cells may be related in part to a phenotypic switch promoted by the host environment and related to quantitative transcriptional or translational changes, resulting in a transient alteration in the concentrations of biologically active products . Thus, both irreversible genetic alterations and transient phenotypic properties contribute to the generation of a malignant phenotype.
Although additional interactions will almost certainly be discovered and the significance of these interactions will be elucidated, it is already at present well accepted that the reactive stroma of cancers is usually associated with increased numbers of fibroblasts, enhanced capillary density and the deposition of a new extracellular matrix that is rich in type-1-collagen and fibrin. Circulating monocytes are also recruited to the reactive stroma in response to the tumoral chemotactic factors and the wound healing-like processes occuring during tumor growth. Tumor-associated macrophages derived from differentiated monocytes and resident macrophages represent the major component of host leukocytes that infiltrate tumor tissues.
This review focuses on the cancer progression towards invasiveness and metastatic spread, taking into consideration the biological role expressed by the so-called "tumor-associated macrophages" (TAM) and "cancer-associated fibroblasts" (CAF).
Tumor cell - macrophage interactions
The monocyte/macrophage lineage constitutes a large portion of tumor infiltrating host cells. They enter into the tumor mass via blood vessels throughout the life span of tumors, from early-stage nodules just beginning to vascularise to late-stage tumors that are invasive and metastatic [31, 32]. A number of tumoral chemoattractants ensure this recruitment, including colony-stimulating factor-1 (CSF-1, also known as M-CSF) [33, 34], CC chemokines  and vascular endothelial growth factor (VEGF) .
Two major lines of evidence connect macrophages and cancer: Firstly the association of chronic inflammation, that leads to macrophage accumulation, with cancer initiation and promotion [15–17]; secondly a high density of TAM correlates with poor prognosis in over 80% of studies . TAM accumulate in critical areas of tumors, such as the hypoxic areas, and hypoxia triggers a pro-angiogenic program in these cells . Hypoxia characterizes the microenvironment of many solid tumors and it has been shown to affect many biological properties of host cells as well as tumor cells that are implicated in tumor growth and metastatic dissemination, e.g. the switch from oxidative to glycolytic metabolism, the production of vascular endothelial growth factors and protease activities [39, 40].
Macrophages are remarkable for the diverse activities in which they can engage on different occasions. Many of these activities appear to be opposing each other: pro-inflammatory vs anti-inflammatory, immunogenic vs tolerogenic, and tissue destructive vs tissue reparative processes. In particular, we know that macrophages from healthy or inflamed tissues are capable of lysing tumor cells, presenting tumor-associated antigens to T-cells and expressing stimulatory cytokines for T- and NK-cells . On the other hand, macrophages isolated from experimental as well as spontaneous tumors show a reduced level of cytotoxic activities [26, 27]. Clearly, macrophages are multifunctional cells that "adapt" themselves to the stimuli that prevail at the site to which they have been attracted . Quiescent macrophages of tissues (resident macrophages) respond to immune or bacterial stimuli by expressing new functional activities, resulting in their capacity to recognize and destroy transformed cells (activated macrophages). During this transition, macrophages may express a number of discrete phenotypic changes characterized by specific functional activities. It is possible, that the contrasting effects exerted by TAM on the growth and metastatic diffusion of tumor cells may reflect different states of activation acquired by macrophages in the tumoral microenvironment. The plasticity of macrophages may be exploited by tumor cells to elicit distinct functions at different stages of tumor progression. It is also possible that multiple subpopulations of TAM exist within a tumor mass, and these may change during tumor development and on the basis of their location.
The contrasting effects of TAM are well exemplified by the macrophage L-arginine metabolism. Macrophages utilize L-arginine to synthesize nitric oxide (NO) with the help of inducible NO synthase (iNOS), and to produce L-ornithine through arginase activity. While NO may contribute to macrophage-driven tumoricidal activities, the polyamines derived from L-ornithine are essential nutrients for tumor cell proliferation . Thus, the balance between iNOS and arginase activity in TAMs might be critical for tumor progression [48, 49]. In addition, arginase can down-regulate NO production by decreasing the intracellular arginine concentration, and low concentrations of NO may act as part of a signalling cascade for neovascularisation . Thus, it was suggested that NO can have pro- or anti-tumor actions, depending on the local concentration of the molecule.
A tumor mass cannot grow beyond 2-3 mm3 in size without angiogenesis. Neovascularization provides an increased supply of nutrients and oxygen, and facilitates the dissemination of tumor cells to distant organs. Most solid tumors pass through two phases of growth: the avascular phase and the vascular phase, when new capillaries penetrate the tumor and it begins to massively grow and invade. Using transgenic mice susceptible to mammary cancer, Lin et al. (2006) demonstrated that a reduction of macrophage infiltration delayed the angiogenic switch and the malignant transition of tumor cells . On the other hand, overexpression of CSF-1 in wild-type mice that leads to an early induction of macrophage infiltration into premalignant lesions accompanied by neoangiogenesis, accelerates their transition to malignancy . Vascular endothelial growth factor (VEGF), a key player in the angiogenesis process, is expressed by both tumor cells and TAM in several histological types of human tumors . In addition, TAM-derived inflammatory cytokines (IL-1β, TNFα) may stimulate tumor cells to enhance the production of VEGF , and to produce angiogenin, a potent proangiogenic protein . VEGF may become also available in the tumor microenvironment through the release of matrix metalloprotease-9 (MMP-9) by TAM .
Recently, Giordano et al. demonstrated that TAM are the most important cell type producing semaphorin 4 D within tumor stroma, a molecule required for angiogenesis and vessel maturation . Therefore, TAM are critical in tumor angiogenesis, an essential step in tumor progression and metastatic dissemination .
Moreover, using a intravital multiphoton microscopy, Wyckoff et al. (2007) observed that tumor cell intravasation in mammary tumors occurs in association with perivascular macrophages , and Ojalvo et al. (2010) demonstrated that this subset of the macrophage population is particularly enriched for molecules involved in Wnt signaling .
Furthermore, tumor cells co-cultivated with macrophages display a higher invasiveness through a TNFα-dependent MMP induction in macrophages . Direct evidence for the role of MMPs in tumor invasiveness has been provided by many studies and cancer cells might stimulate TAM to produce MMPs in a paracrine manner through the secretion of various stimuli, such as interleukins, growth factors and an extracellular matrix metalloproteinase inducer (CD147). The invasiveness of tumor cells is also stimulated by epidermal growth factor (EGF) synthesized by TAM in response to tumor-derived CSF-1, leading to the induction of several genes involved in the migration of tumor cells .
The leading edge of a tumor mass is the site where TAM direct the invasion of tumor cells into host tissues. With melanoma cells, we found that the areas of greatest macrophage density were peritumoral [63, 64], and using a suitable in vitro model we demonstrated that upon contact with melanoma cells, inflammatory macrophages express increased levels of COX-2 , uPAR and MMP-9 . It is also possible that MMPs secreted by TAM can be recruited to cancer cell membranes, and are then used as tools by the tumor cells to progress through a specific site . Hiratsuka et al. (2002) proved that MMP-9 expressed in alveolar endothelial cells and macrophages renders the pulmonary metastatic site fertile for secondary growth of malignant cells, a mechanism dependent upon the activation of the VEGF-VEGFR signaling cascade . TAM also produce, under the influence of tumor cells, the urokinase plasminongen activator (uPA) and receptor (uPAR), that may cause degradation of ECM to promote invasion and spread of tumor cells [68, 69]. The uPA/uPAR system does not only support the invasion of tumor cells, it also modulates cell adhesion by interactions of uPAR with vitronectin and integrins. Therefore, the uPA/uPAR system is endowed with the structural and functional properties required to promote most important mechanisms of tumor cell migration [68, 69].
Genetic experiments provide a causal link between CSF-1-dependent TAM and malignancy in mammary and lung cancer . In particular, crossing transgenic mice susceptible to mammary cancer and mice containing a recessive null mutation in the CSF-1 gene, Lin et al. demonstrated that TAM are necessary for distant organ colonization, the final step of metastatic dissemination .
Important recent studies suggest that macrophages are recruited into the target organs and facilitate metastatic cell seeding. Moreover, blocking macrophage lodgement at the metastatic site limits the growth of metastatic cells, even if metastatic lesions have readily been established .
Thus, TAM are powerful tumor promoters, capable to stimulate angiogenesis, invasiveness and subsequent metastatic growth, but also able to set up the sites for metastatic cell seeding.
Tumor cell - fibroblast interactions
Regarding secreted factors that affect tumor cell invasiveness, myofibroblasts are a source of extracellular matrix-degrading proteases such as the MMPs. In particular, MMP-3, also known as stromelysin 1, is highly expressed in fibroblasts/myofibroblasts and participates in the cleavage of E-cadherin, thereby prompting epithelial cancer cells to perform an epithelial-mesenchymal transition (EMT) . Cancer cells undergoing EMT loose cell-cell contacts, acquire mesenchymal properties and develop invasive and migratory abilities. Consequently, EMT of cancer cells is recognized as an important determinant of tumor progression .
By injecting tumor cell/fibroblast cell suspension into immunodeficent mice, Orimo et al. found that CAF isolated from a human breast carcinoma and expressing a myofibroblast phenotype, promote the growth of carcinoma cells through a stromal cell-derived factor-1 [SDF-1]/CXCR4-dependent mechanism. These CAF did not show aneuploidy or in vivo tumorigenic activity . Moreover, mammary carcinoma-associated fibroblasts stimulate a high vasculature by recruiting endothelial progenitor cells in tumor xenografts. Thus, SDF-1 secreted by mammary myofibroblasts may stimulate the growth of CXCR4-expressing carcinoma cells as well as angiogenesis . A genome analysis of the stroma of an elevated number of invasive breast carcinoma indicates that the hot spots for mutations in the stroma are not the same as those identified in the epithelium . Therefore, it is possible that an independent pathway of mutation of gene expression works in stromal cells. Recently, Studebaker et al. showed that IL-6 secreted by CAF enhances the growth and invasiveness of estrogen receptor α-positive breast carcinoma cells through its effectors, Notch-3, Jagged-1 and carbonic anhydrase IX .
Moreover, using an in vitro model of skin carcinogenesis, Cat et al.  demonstrated that tumor cell-derived TGF-β stimulates reactive oxygen species-dependent expression of α-SMA in skin fibroblasts, and their differentiation into myofibroblasts. This was associated with an increased release of hepatocyte growth factor (HGF), VEGF and IL-6. In view of the notion that skin fibroblasts possess a reduced capacity to secrete IL-6, while senescent fibroblasts strongly up-regulate IL-6 and stimulate malignancy in epithelial cells , it was suggested that CAF may represent a subset of senescent fibroblasts. Importantly, a very recent report demonstrates that CAF isolated from dysplastic skin and skin carcinoma express a NF-κB-dependent proinflammatory gene signature responsible for macrophage recruitment, neovascularisation, cancer cell proliferation and invasion. This was also manifest in CAF of mouse and human mammary and pancreatic tumors .
It is possible that the most important feature in progression of prostate tumors is the ability of tumor cells to stimulate stromal cells to release biological agents for their growth and dissemination. Mutual interactions between carcinoma cells and CAF were reported by Nakamura et al.: tumoral IL-1, basic fibroblast growth factor (bFGF) and platelet-derived growth factor (PDGF) stimulate HGF expression in CAF, and in turn, stromal HGF leads to an invasive phenotype in carcinoma cells . Hill et al., using a mouse model of prostate carcinoma, showed that tumor cells upregulate p53 in stromal fibroblasts, a process found to induce a selection of a subpopulation of p53 null fibroblasts. In turn, selection of a p53 null subpopulation of stromal fibroblasts contributed to the progression of carcinoma cells .
The origins of CAF were revised by Orimo and Weinberg, who suggested three major alternative hypotheses: a) genetic alteration, b) an activation without genetic alteration of normal tissue fibroblasts, and c) the activation of bone marrow-derived mesenchymal stem cells (MSC) . MSC infiltrate wounds and tumors in high numbers, and, when co-injected into immunodeficient hosts together with weakly metastatic human breast carcinoma cells, they induced in these cancer cells an increase in metastatic potential by a CCL5/CCR5-dependent mechanism .
Despite these initial findings, additional efforts at determining the molecular mechanisms that lead to the appearance of differentiated fibroblasts and their multiple contributions in tumor progression are still urgently required.
Tumor stroma is a specialized form of tissue composed of host cells and signals of different origin, which is associated with tumor cell growth, primarily of epithelial origin. Tumor stroma possesses unique structural features that differ from the native stroma. It is also characterised by a great degree of tumor dependency ("There is no tumor stroma without a tumor") and displays a substantial degree of plasticity, with the specific outcomes controlled by tumor cells themselves. Indeed, tumor cells do not only display heterogeneity and induce the expression of signaling molecules that favour their survival and invasiveness into local and distant host tissues, but also influence host stromal elements to produce relevant effectors that act as tumor promoters. Moreover, tumor cell-derived signals recruit and activate some host cells, among which monocytes/macrophages and fibroblasts are the most abundant population within the tumor microenvironment. As was discussed above, both types of cells, macrophages and fibroblasts, are involved in a intricate liaison with tumor cells, that usually leads to tumor progression and activation of the metastatic cascade. Thus, the investigation of the mechanisms that allow macrophages and fibroblasts to contribute to tumor progression, could lead to new approaches for the anti-cancer therapies that are urgently required.
This study was funded by grants from Progetto di Ricerca di Ateneo Quota ex-60%, Istituto Toscano Tumori, Ente Cassa di Risparmio di Firenze and Lucca. The authors wish to thank Eugenio Torre for his valuable assistance in the preparation of histology slides and the CCS staff for editorial support.
- Gupta GP, Massague J: Cancer metastasis: building a framework. Cell. 2006, 127: 679-695. 10.1016/j.cell.2006.11.001.View ArticlePubMedGoogle Scholar
- Sahai E: Illuminating the metastatic process. Nat Rev Cancer. 2007, 7: 737-749. 10.1038/nrc2229.View ArticlePubMedGoogle Scholar
- Nguyen DX, Bos PD, Massagué J: Metastasis: from dissemination to organ-specific colonization. Nat Rev Cancer. 2009, 9: 274-284. 10.1038/nrc2622.View ArticlePubMedGoogle Scholar
- Heppner GH, Miller FR: The cellular basis of tumor progression. Int Rev Cytol. 1998, 177: 1-56. 10.1016/S0074-7696(08)62230-5.View ArticlePubMedGoogle Scholar
- Fidler IJ: Tumor heterogeneity and the biology of cancer invasion and metastasis. Cancer Res. 1978, 38: 2651-2660.PubMedGoogle Scholar
- Foulds L: The experimental study of tumor progression: a review. Cancer Res. 1954, 14: 327-339.PubMedGoogle Scholar
- Nicolson GL: Tumor progression, oncogenes and the evolution of metastatic phenotypic diversity. Clin Exp Metastasis. 1984, 2: 85-105. 10.1007/BF00052411.View ArticlePubMedGoogle Scholar
- Miller FR, Heppner GH: Cellular interactions in metastasis. Cancer Metastasis Rev. 1990, 9: 21-34. 10.1007/BF00047586.View ArticlePubMedGoogle Scholar
- Witz IP: Tumor-microenvironment interactions: dangerous liaisons. Adv Cancer Res. 2008, 100: 203-29. full_text.View ArticlePubMedGoogle Scholar
- Rose DP, Connolly JM, Meschter CL: Effect of dietary fat on human breast cancer growth and lung metastasis in nude mice. J Natl Cancer Inst. 1991, 83: 1491-1495. 10.1093/jnci/83.20.1491.View ArticlePubMedGoogle Scholar
- Rose DP, Connolly JM, Liu XH: Effects of linoleic acid on the growth and metastasis of two human breast cancer cell lines in nude mice and the invasive capacity of these cell lines in vitro. Cancer Res. 1994, 54: 6557-6562.PubMedGoogle Scholar
- Mannini A, Calzolari A, Calorini L, Mugnai G, Ruggieri S: The inhibition of lung colonization of B16-F10 melanoma cells in EFA-deficient animals is related to enhanced apoptosis and reduced angiogenesis. Clin Exp Metastasis. 2006, 23: 1591-65. 10.1007/s10585-006-9022-1.View ArticleGoogle Scholar
- Bingham S, Riboli E: Diet and cancer - the European Prospective Investigation into Cancer and Nutrition. Nat Rev Cancer. 2004, 4: 3:206-215. 10.1038/nrc1298.View ArticlePubMedGoogle Scholar
- Giraldi T, Perissin L, Zorzet S, Rapozzi V: Metastasis and neuroendocrine system in stressed mice. Ann N Y Acad Sc. 1992, 650: 297-301. 10.1111/j.1749-6632.1992.tb49140.x.View ArticleGoogle Scholar
- Coussens LM, Werb Z: Inflammation and cancer. Nature. 2002, 420: 860-867. 10.1038/nature01322.PubMed CentralView ArticlePubMedGoogle Scholar
- Robinson SC, Coussens LM: Soluble mediators of inflammation during tumor development. Adv Cancer Res. 2005, 93: 159-187. 10.1016/S0065-230X(05)93005-4.View ArticlePubMedGoogle Scholar
- Mantovani A, Allavena P, Sica A, Balkwill F: Cancer-related inflammation. Nature. 2008, 454: 436-44. 10.1038/nature07205.View ArticlePubMedGoogle Scholar
- Gasic GJ: Role of plasma, platelets and endothelial cells in tumor metastasis. Cancer Metastasis Rev. 1986, 3: 99-116.View ArticleGoogle Scholar
- de Visser KE, Korets LV, Coussens LM: De novo carcinogenesis promoted by chronic inflammation is B lymphocyte dependent. Cancer Cell. 2005, 7: 411-423. 10.1016/j.ccr.2005.04.014.View ArticlePubMedGoogle Scholar
- Di Carlo E, Forni G, Lollini P, Colombo MP, Modesti A, Musiani P: The intriguing role of polymorphonuclear neutrophils in antitumor reactions. Blood. 2001, 97: 339-345. 10.1182/blood.V97.2.339.View ArticlePubMedGoogle Scholar
- De Larco JE, Wuertz BR, Furcht LT: The potential role of neutrophils in promoting the metastatic phenotype of tumors releasing interleukin-8. Clin Cancer Res. 2004, 10: 4895-4900. 10.1158/1078-0432.CCR-03-0760.View ArticlePubMedGoogle Scholar
- Silzle T, Randolph GJ, Kreutz M, Kunz-Schughart LA: The fibroblast: sentinel cell and local immune modulator in tumor tissue. Int J Cancer. 2004, 108: 173-180. 10.1002/ijc.11542.View ArticlePubMedGoogle Scholar
- Kalluri R, Zeisberg M: Fibroblasts in cancer. Nat Rev Cancer. 2006, 6: 392-401. 10.1038/nrc1877.View ArticlePubMedGoogle Scholar
- Shimoda Masayuki, Mellodyb Kieran, Orimo Akira: Carcinoma-associated fibroblasts are a rate-limiting determinant for tumour progression. Semin Cell Dev Biol. 2010, 21: 19-25. 10.1016/j.semcdb.2009.10.002.PubMed CentralView ArticlePubMedGoogle Scholar
- Grégoire M, Lieubeau B: The role of fibroblasts in tumor behaviour. Cancer Metastasis Rev. 1995, 14: 339-350. 10.1007/BF00690602.View ArticlePubMedGoogle Scholar
- Qian BZ, Pollard JW: Macrophage diversity enhances tumor progression and metastasis. Cell. 2010, 141: 39-51. 10.1016/j.cell.2010.03.014.View ArticlePubMedGoogle Scholar
- Mantovani A: La mala educación of tumor-associated macrophages: Diverse pathways and new players. Cancer Cell. 2010, 17: 111-112. 10.1016/j.ccr.2010.01.019.View ArticlePubMedGoogle Scholar
- Rubin H: Contact interactions between cells that suppress neoplastic development: can they also explain metastatic dormancy?. Adv Cancer Res. 2008, 100: 159-202. full_text.View ArticlePubMedGoogle Scholar
- Aguirre-Ghiso JA: Models, mechanisms and clinical evidence for cancer dormancy. Nat Rev Cancer. 2007, 7: 834-846. 10.1038/nrc2256.PubMed CentralView ArticlePubMedGoogle Scholar
- Nicolson GL: Cancer progression and growth: relationship of paracrine and autocrine growth mechanisms to organ preference of metastasis. Exp Cell Res. 1993, 204: 171-80. 10.1006/excr.1993.1022.View ArticlePubMedGoogle Scholar
- Normann SJ: Macrophage infiltration and tumor progression. Cancer Metastasis Rev. 1985, 4: 277-291. 10.1007/BF00048093.View ArticlePubMedGoogle Scholar
- Bugelski PJ, Corwin SP, North SM, Kirsh RL, Nicolson GL, Poste G: Macrophage content of spontaneous metastases at different stages of growth. Cancer Res. 1987, 4141-4145. 47PubMedGoogle Scholar
- Nowicki A, Szenajch J, Ostrowska G, Wojtowicz A, Wojtowicz K, Kruszewski AA, Maruszynski M, Aukerman SL, Wiktor-Jedrzejczak W: Impaired tumor growth in colony-stimulating factor 1 (CSF-1)-deficient, macrophage-deficient op/op mouse: evidence for a role of CSF-1-dependent macrophages in formation of tumor stroma. Int J Cancer. 1996, 65: 112-119. 10.1002/(SICI)1097-0215(19960103)65:1<112::AID-IJC19>3.0.CO;2-I.View ArticlePubMedGoogle Scholar
- Lin EY, Nguyen AV, Russel RG, Pollard JW: Colony-stimulating factor promotes progression of mammary tumors to malignancy. J Exp Med. 2001, 193: 727-740. 10.1084/jem.193.6.727.PubMed CentralView ArticlePubMedGoogle Scholar
- Mantovani A, Allavena P, Sozzani S, Vecchi A, Locati M, Sica A: Chemokines in the recruitment and shaping of the leukocyte infiltrate of tumors. Semin Cancer Biol. 2004, 14: 155-160. 10.1016/j.semcancer.2003.10.001.View ArticlePubMedGoogle Scholar
- Barleon B, Sozzani S, Zhou D, Weich HA, Mantovani A, Marmé D: Migration of human monocytes in response to vascular endothelial growth factor (VEGF) is mediated via the VEGF receptor flt-1. Blood. 1996, 87: 3336-3343.PubMedGoogle Scholar
- Lin EY, Gouon-Evans V, Nguyen AV, Pollard JW: The macrophage growth factor CSF-1 in mammary gland development and tumor progression. J Mammary Gland Biol Neoplasia. 2002, 7: 147-162. 10.1023/A:1020399802795.View ArticlePubMedGoogle Scholar
- Murdoch C, Giannoudis A, Lewis CE: Mechanisms regulating the recruitment of macrophages into hypoxic areas of tumors and other ischemic tissues. Blood. 2004, 104: 2224-2234. 10.1182/blood-2004-03-1109.View ArticlePubMedGoogle Scholar
- Chan DA, Giaccia AJ: Hypoxia, gene expression, and metastasis. Cancer Metastasis Rev. 2007, 26: 333-339. 10.1007/s10555-007-9063-1.View ArticlePubMedGoogle Scholar
- Keith B, Simon MC: Hypoxia-inducible factors, stem cells, and cancer. Cell. 2007, 129: 465-472. 10.1016/j.cell.2007.04.019.PubMed CentralView ArticlePubMedGoogle Scholar
- Fidler IJ, Schroit AJ: Recognition and destruction of neoplastic cells by activated macrophages: discrimination of altered self. Biochim Biophys Acta. 1998, 948: 151-73.Google Scholar
- Gordon S, Taylor PR: Monocyte and macrophage heterogeneity. Nat Rev Immunol. 2005, 5: 953-964. 10.1038/nri1733.View ArticlePubMedGoogle Scholar
- Mantovani A, Sozzani S, Locati M, Allavena P, Sica A: Macrophage polarization:tumor-associated macrophages as a paradigm for polarized M2 mononuclear phagocytes. Trends Immunol. 2002, 23: 549-555. 10.1016/S1471-4906(02)02302-5.View ArticlePubMedGoogle Scholar
- Allavena P, Sica A, Garlanda C, Mantovani A: The Yin-Yang of tumor-associated macrophages in neoplastic progression and immune surveillance. Immunol Rev. 2008, 222: 155-161. 10.1111/j.1600-065X.2008.00607.x.View ArticlePubMedGoogle Scholar
- Biswas SK, Gangi L, Paul S, Schioppa T, Saccani A, Sironi M, Bottazzi B, Doni A, Vincenzo B, Pasqualini F, Vago L, Nebuloni M, Mantovani A, Sica A: A distinct and unique transcriptional program expressed by tumor-associated macrophages (defective NF-kappaB and enhanced IRF-3/STAT1 activation). Blood. 2006, 107: 2112-2122. 10.1182/blood-2005-01-0428.View ArticlePubMedGoogle Scholar
- Sica A, Bronte V: Altered macrophage differentiation and immune dysfunction in tumor development. J Clin Invest. 2007, 117: 1155-1166. 10.1172/JCI31422.PubMed CentralView ArticlePubMedGoogle Scholar
- Auvinen M, Paasinen A, Andersson LC, Holtta E: Ornithine decarboxylase activity is critical for cell transformation. Nature. 1992, 360: 355-358. 10.1038/360355a0.View ArticlePubMedGoogle Scholar
- Mills CD, Shearer J, Evans R, Caldwell MD: Macrophage arginine metabolism and the inhibition or stimulation of cancer. J Immunol. 1992, 149: 2709-2714.PubMedGoogle Scholar
- Chang CI, Liao JC, Kuo L: Macrophage arginase promotes tumor cell growth and suppresses nitric oxide-mediated tumor cytotoxicity. Cancer Res. 2001, 61: 1100-1106.PubMedGoogle Scholar
- Fukumura D, Jain RK: Role of nitric oxide in angiogenesis and microcirculation in tumors. Cancer Metastasis Rev. 1998, 17: 77-89. 10.1023/A:1005908805527.View ArticlePubMedGoogle Scholar
- Lin EY, Li JF, Gnatovskiy L, Deng Y, Zhu L, Grzesik DA, Qian H, Xue XN, Pollard JW: Macrophages regulate the angiogenic switch in a mouse model of breast cancer. Cancer Res. 2006, 66: 11238-11246. 10.1158/0008-5472.CAN-06-1278.View ArticlePubMedGoogle Scholar
- Lin EY, Pollard JW: Tumor-associated macrophages press the angiogenic switch in breast cancer. Cancer Res. 2007, 67: 5064-6. 10.1158/0008-5472.CAN-07-0912.View ArticlePubMedGoogle Scholar
- Leek RD, Harris AL, Lewis CE: Cytokine networks in solid human tumors: regulation of angiogenesis. J Leukoc Biol. 1994, 56: 423-35.PubMedGoogle Scholar
- Torisu H, Ono M, Kiryu H, Furue M, Ohmoto Y, Nakayama J, Nishioka Y, Sone S, Kuwano M: Macrophage infiltration correlates with tumor stage and angiogenesis in human malignant melanoma: possible involvement of TNFalpha and IL-1alpha. Int J Cancer. 2000, 85: 182-8.View ArticlePubMedGoogle Scholar
- Etoh T, Shibuta K, Barnard GF, Kitano S, Mori M: Angiogenin expression in human colorectal cancer: the role of focal macrophage infiltration. Clin Cancer Re. 2000, 6: 3545-51.Google Scholar
- Bergers G, Brekken R, McMahon G, Vu TH, Itoh T, Tamaki K, Tanzawa K, Thorpe P, Itohara S, Werb Z, Hanahan D: Matrix metalloproteinase-9 triggers the angiogenic switch during carcinogenesis. Nat Cell Biol. 2000, 2: 737-44. 10.1038/35036374.PubMed CentralView ArticlePubMedGoogle Scholar
- Sierra JR, Corso S, Caione L, Cepero V, Conrotto P, Cignetti A, Piacibello W, Kumanogoh A, Kikutani H, Comoglio PM, Tamagnone L, Giordano S: Tumor angiogenesis and progression are enhanced by Sema4 D produced by tumor-associated macrophages. J Exp Med. 2008, 205: 1673-1685. 10.1084/jem.20072602.PubMed CentralView ArticlePubMedGoogle Scholar
- Carmeliet P, Jain RK: Angiogenesis in cancer and other diseases. Nature. 2000, 407: 249-257. 10.1038/35025220.View ArticlePubMedGoogle Scholar
- Wyckoff JB, Wang Y, Lin EY, Li JF, Goswami S, Stanley ER, Segall JE, Pollard JW, Condeelis J: Direct visualization of macrophage-assisted tumor cell intravasation in mammary tumors. Cancer Res. 2007, 67: 2649-2656. 10.1158/0008-5472.CAN-06-1823.View ArticlePubMedGoogle Scholar
- Ojalvo LS, Whittaker CA, Condeelis JS, Pollard JW: Gene expression analysis of macrophages that facilitate tumor invasion supports a role for Wnt-signaling in mediating their activity in primary mammary tumors. J Immunol. 2010, 184: 702-712. 10.4049/jimmunol.0902360.PubMed CentralView ArticlePubMedGoogle Scholar
- Pollard JW: Tumour-educated macrophages promote tumour progression and metastasis. Nat Rev Cancer. 2004, 4: 71-78. 10.1038/nrc1256.View ArticlePubMedGoogle Scholar
- Goswami S, Sahai E, Wyckoff JB, Cammer M, Cox D, Pixley FJ, Stanley ER, Segall JE, Condeelis JS: Macrophages promote the invasion of breast carcinoma cells via a colony-stimulating factor-1/epidermal growth factor paracrine loop. Cancer Res. 2005, 65: 5278-5283. 10.1158/0008-5472.CAN-04-1853.View ArticlePubMedGoogle Scholar
- Massi D, Marconi C, Franchi A, Bianchini F, Paglierani M, Ketabchi S, Miracco C, Santucci M, Calorini L: Arginine metabolism in tumor-associated macrophages in cutaneous malignant melanoma: evidence from human and experimental tumors. Hum Pathol. 2007, 38: 1516-1525. 10.1016/j.humpath.2007.02.018.View ArticlePubMedGoogle Scholar
- Bianchini F, Massi D, Marconi C, Franchi A, Baroni G, Santucci M, Mannini A, Mugnai G, Calorini L: Expression of cyclo-oxygenase-2 in macrophages associated with cutaneous melanoma at different stages of progression. Prostaglandins Other Lipid Mediat. 2007, 83: 320-328. 10.1016/j.prostaglandins.2007.03.003.View ArticlePubMedGoogle Scholar
- Bianchini F, D'Alessio S, Fibbi G, Del Rosso M, Calorini L: Cytokine-dependent invasiveness in B16 murine melanoma cells: role of uPA system and MMP-9. Oncol Rep. 2006, 15: 709-714.PubMedGoogle Scholar
- Egeblad M, Werb Z: New functions for the matrix metalloproteinases in cancer progression. Nat Rev Cancer. 2002, 2: 161-174. 10.1038/nrc745.View ArticlePubMedGoogle Scholar
- Hiratsuka S, Nakamura K, Iwai S, Murakami M, Itoh T, Kijima H, Shipley JM, Senior RM, Shibuya M: MMP9 induction by vascular endothelial growth factor receptor-1 is involved in lung-specific metastasis. Cancer Cell. 2002, 2: 289-300. 10.1016/S1535-6108(02)00153-8.View ArticlePubMedGoogle Scholar
- Del Rosso M, Fibbi G, Pucci M, D'Alessio S, Del Rosso A, Magnelli L, Chiarugi V: Multiple pathways of cell invasion are regulated by multiple families of serine proteases. Clin Exp Metastasis. 2002, 19: 193-207. 10.1023/A:1015531321445.View ArticlePubMedGoogle Scholar
- Sidenius N, Blasi F: The urokinase plasminogen activator system in cancer: recent advances and implication for prognosis and therapy. Cancer Metastasis Rev. 2003, 22: 205-222. 10.1023/A:1023099415940.View ArticlePubMedGoogle Scholar
- Lin EY, Nguyen AV, Russell RG, Pollard JW: Colony-stimulating factor 1 promotes progression of mammary tumors to malignancy. J Exp Med. 2001, 193: 727-740. 10.1084/jem.193.6.727.PubMed CentralView ArticlePubMedGoogle Scholar
- Lin EY, Jones JG, Li P, Zhu L, Whitney KD, Muller WJ, Pollard JW: Progression to malignancy in the polyoma middle T oncoprotein mouse breast cancer model provides a reliable model for human diseases. Am J Pathol. 2003, 163: 2113-2126.PubMed CentralView ArticlePubMedGoogle Scholar
- Cecconi O, Calorini L, Mannini A, Mugnai G, Ruggieri S: Enhancement of lung-colonizing potential of murine tumor cell lines co-cultivated with activated macrophages. Clin Exp Metastasis. 1997, 15: 94-101. 10.1023/A:1018440508189.View ArticlePubMedGoogle Scholar
- Calorini L, Mannini A, Bianchini F, Mugnai G, Balzi M, Becciolini A, Ruggieri S: Biological properties associated with the enhanced lung-colonizing potential in a B16 murine melanoma line grown in a medium conditioned by syngeneic Corynebacterium parvum-elicited macrophages. Clin Exp Metastasis. 1999, 17: 889-895. 10.1023/A:1006783431599.View ArticlePubMedGoogle Scholar
- Calorini L, Bianchini F, Mannini A, Mugnai G, Balzi M, Becciolini A, Ruggieri S: IFN gamma and TNF alpha account for a pro-clonogenic activity secreted by activated murine peritoneal macrophages. Clin Exp Metastasis. 2002, 19: 259-264. 10.1023/A:1015583322354.View ArticlePubMedGoogle Scholar
- Qian B, Deng Y, Im JH, Muschel RJ, Zou Y, Li J, Lang RA, Pollard JW: A distinct macrophage population mediates metastatic breast cancer cell extravasation, establishment and growth. PLoS One. 2009, 4: e6562-10.1371/journal.pone.0006562.PubMed CentralView ArticlePubMedGoogle Scholar
- Micke P, Ostman A: Tumour-stroma interaction: cancer-associated fibroblasts as novel targets in anti-cancer therapy?. Lung Cancer. 2004, 45 (Suppl 2): S163-75. 10.1016/j.lungcan.2004.07.977.View ArticlePubMedGoogle Scholar
- Kalluri R, Zeisberg M: Fibroblasts in cancer. Nat Rev Cancer. 2006, 6: 392-401. 10.1038/nrc1877.View ArticlePubMedGoogle Scholar
- Dvorak HF: Tumors: wounds that do not heal. Similarities between tumor stroma generation and wound healing. N Engl J Med. 1986, 315: 1650-1659. 10.1056/NEJM198612253152606.View ArticlePubMedGoogle Scholar
- Dingemans KP, Zeeman-Boeschoten IM, Keep RF, Das PK: Transplantation of colon carcinoma into granulation tissue induces an invasive morphotype. Int J Cancer. 1993, 54: 1010-1016. 10.1002/ijc.2910540625.View ArticlePubMedGoogle Scholar
- Hinz B, Phan SH, Thannickal VJ, Galli A, Bochaton-Piallat ML, Gabbiani G: The myofibroblast: one function, multiple origins. Am J Pathol. 2007, 170: 1807-1816. 10.2353/ajpath.2007.070112.PubMed CentralView ArticlePubMedGoogle Scholar
- Shimoda M, Mellody KT, Orimo A: Carcinoma-associated fibroblasts are a rate-limiting determinant for tumour progression. Semin Cell Dev Biol. 2010, 21: 19-25. 10.1016/j.semcdb.2009.10.002.PubMed CentralView ArticlePubMedGoogle Scholar
- Lochter A, Galosy S, Muschler J, Freedman N, Werb Z, Bissell MJ: Matrix metalloproteinase stromelysin-1 triggers a cascade of molecular alterations that leads to stable epithelial-to-mesenchymal conversion and a premalignant phenotype in mammary epithelial cells. J Cell Biol. 1997, 139: 1861-1872. 10.1083/jcb.139.7.1861.PubMed CentralView ArticlePubMedGoogle Scholar
- Polyak K, Weinberg RA: Transitions between epithelial and mesenchymal states: acquisition of malignant and stem cell traits. Nat Rev Cancer. 2009, 9: 265-273. 10.1038/nrc2620.View ArticlePubMedGoogle Scholar
- Orimo A, Gupta PB, Sgroi DC, Arenzana-Seisdedos F, Delaunay T, Naeem R, Carey VJ, Richardson AL, Weinberg RA: Stromal fibroblasts present in invasive human breast carcinomas promote tumor growth and angiogenesis through elevated SDF-1/CXCL12 secretion. Cell. 2005, 121: 335-348. 10.1016/j.cell.2005.02.034.View ArticlePubMedGoogle Scholar
- Fukino K, Shen L, Matsumoto S, Morrison CD, Mutter GL, Eng C: Combined total genome loss of heterozygosity scan of breast cancer stroma and epithelium reveals multiplicity of stromal targets. Cancer Res. 2004, 64: 7231-7236. 10.1158/0008-5472.CAN-04-2866.View ArticlePubMedGoogle Scholar
- Studebaker AW, Storci G, Werbeck JL, Sansone P, Sasser AK, Tavolari S, Huang T, Chan MW, Marini FC, Rosol TJ, Bonafé M, Hall BM: Fibroblasts isolated from common sites of breast cancer metastasis enhance cancer cell growth rates and invasiveness in an interleukin-6-dependent manner. Cancer Res. 2008, 68: 9087-9095. 10.1158/0008-5472.CAN-08-0400.View ArticlePubMedGoogle Scholar
- Cat B, Stuhlmann D, Steinbrenner H, Alili L, Holtkötter O, Sies H, Brenneisen P: Enhancement of tumor invasion depends on transdifferentiation of skin fibroblasts mediated by reactive oxygen species. J Cell Sci. 2006, 119: 2727-38. 10.1242/jcs.03011.View ArticlePubMedGoogle Scholar
- Krtolica A, Parrinello S, Lockett S, Desprez PY, Campisi J: Senescent fibroblasts promote epithelial cell growth and tumorigenesis: a link between cancer and aging. Proc Natl Acad Sci USA. 2001, 98: 12072-12077. 10.1073/pnas.211053698.PubMed CentralView ArticlePubMedGoogle Scholar
- Erez N, Truitt M, Olson P, Hanahan D: Cancer-Associated Fibroblasts are activated in incipient neoplasia to orchestrate tumor-promoting inflammation in an NF-kappaB-dependent manner. Cancer Cell. 2010, 17: 135-47. 10.1016/j.ccr.2009.12.041.View ArticlePubMedGoogle Scholar
- Chung LW, Baseman A, Assikis V, Zhau HE: Molecular insights into prostate cancer progression: the missing link of tumor microenvironment. J Urol. 2005, 173: 10-20. 10.1097/01.ju.0000141582.15218.10.View ArticlePubMedGoogle Scholar
- Chung LW, Chang SM, Bell C, Zhau HE, Ro JY, von Eschenbach AC: Co-inoculation of tumorigenic rat prostate mesenchymal cells with non-tumorigenic epithelial cells results in the development of carcinosarcoma in syngeneic and athymic animals. Int J Cancer. 1989, 43: 1179-1187. 10.1002/ijc.2910430636.View ArticlePubMedGoogle Scholar
- Tuxhorn JA, Ayala GE, Smith MJ, Smith VC, Dang TD, Rowley DR: Reactive stroma in human prostate cancer: induction of myofibroblast phenotype and extracellular matrix remodeling. Clin Cancer Res. 2002, 8: 2912-2923.PubMedGoogle Scholar
- Olumi AF, Grossfeld GD, Hayward SW, Carroll PR, Tlsty TD, Cunha GR: Carcinoma-associated fibroblasts direct tumor progression of initiated human prostatic epithelium. Cancer Res. 1999, 59: 5002-5011.PubMedGoogle Scholar
- Nakamura T, Matsumoto K, Kiritoshi A, Tano Y, Nakamura T: Induction of hepatocyte growth factor in fibroblasts by tumor-derived factors affects invasive growth of tumor cells: in vitro analysis of tumor-stromal interactions. Cancer Res. 1997, 57: 3305-3313.PubMedGoogle Scholar
- Hill R, Song Y, Cardiff RD, Van Dyke T: Selective evolution of stromal mesenchyme with p53 loss in response to epithelial tumorigenesis. Cell. 2005, 123: 1001-1011. 10.1016/j.cell.2005.09.030.View ArticlePubMedGoogle Scholar
- Orimo A, Weinberg RA: Heterogeneity of stromal fibroblasts in tumors. Cancer Biol Ther. 2007, 6: 618-619.View ArticlePubMedGoogle Scholar
- Karnoub AE, Dash AB, Vo AP, Sullivan A, Brooks MW, Bell GW, Richardson AL, Polyak K, Tubo R, Weinberg RA: Mesenchymal stem cells within tumour stroma promote breast cancer metastasis. Nature. 2007, 449: 557-63. 10.1038/nature06188.View ArticlePubMedGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.