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Peroxiredoxin 6 promotes upregulation of the prion protein (PrP) in neuronal cells of prion-infected mice
Cell Communication and Signaling volume 10, Article number: 38 (2012)
It has been widely established that the conversion of the cellular prion protein (PrPC) into its abnormal isoform (PrPSc) is responsible for the development of transmissible spongiform encephalopathies (TSEs). However, the knowledge of the detailed molecular mechanisms and direct functional consequences within the cell is rare. In this study, we aimed at the identification of deregulated proteins which might be involved in prion pathogenesis.
Apolipoprotein E and peroxiredoxin 6 (PRDX6) were identified as upregulated proteins in brains of scrapie-infected mice and cultured neuronal cell lines. Downregulation of PrP gene expression using specific siRNA did not result in a decrease of PRDX6 amounts. Interestingly, selective siRNA targeting PRDX6 or overexpression of PRDX6 controlled PrPC and PrPSc protein amounts in neuronal cells.
Besides its possible function as a novel marker protein in the diagnosis of TSEs, PDRX6 represents an attractive target molecule in putative pharmacological intervention strategies in the future.
Transmissible spongiform encephalopathies (TSEs) are fatal neurodegenerative disorders, which include scrapie in sheep, bovine spongiform encephalopathy (BSE) in cattle, and Creutzfeldt-Jacob disease (CJD) in humans. The molecular hallmark of these disorders is a structural conversion in folding of the normal cellular prion protein (PrPC) into a disease-associated, protease-resistant isoform (PrPSc). Neuropathological characteristics of these diseases include neuronal loss, vacuolar degeneration, astrogliosis and amyloid plaque formation caused by accumulation of PrPSc. However, the mechanism whereby PrPC → PrPSc conversion triggers cellular neurotoxicity and neurodegeneration is not well understood.
PrPC is a multifunctional plasma membrane glycosylphosphatidylinositol (GPI)-anchored protein on a wide range of different cell types where it is involved in adhesion, signal transduction, differentiation, survival or stress protection[4–6]. Obviously, neurodegenerative disorders interconnect several cellular signal transduction pathways to cause oxidative stress in the brain, including increased oxidative damage, impaired mitochondrial function, defects of the proteasome system, the presence of aggregated proteins, and many more. There are a number of cellular antioxidant defenses to convert reactive oxygen species (ROS) into unreactive compounds, e.g. superoxide dismutase (SOD) and the peroxiredoxin (PRDX) family. Proteins of the PRDX family are widely expressed and exhibit crucial protective functions in neurological disorders such as Parkinson's and Alzheimer's diseases. Accordingly, upregulation of PRDX protein was observed in the brain of patients with Parkinson and Alzheimer’s disease, and also during experimental prion infection in mice[9–11]. The PRDX family of mammals comprises six isoforms (PRDX1-6), which are classified into the three subgroups typical 2-Cys PRDX (PRDX1–4), atypical 2-Cys PRDX (PRDX5) and 1-Cys PRDX (PRDX6). Peroxiredoxin 6 is the only 1-Cys member and exhibits bifunctional activities as a glutathione (GSH) peroxidase and calcium-independent phospholipase A2 (PLA2), which might contribute to neurological disorders. In experimental in vivo models for neurological disorders such as Huntington′s disease and scrapie, PRDX1 was slightly enhanced. However, the function of distinct PRDX isoforms in prion diseases has not been investigated.
Upregulation of PRDX6 in scrapie-infected brains
For a better understanding of the proteomic alterations during in vivo prion pathogenesis, C57Bl/6 mice were intracerebrally inoculated with a 10% brain homogenate containing the prion strain 139A (Additional file1). Mice were sacrificed after 40, 80, 120 and 150 days, and brain homogenates were prepared. To confirm prion infection, PrP expression was analyzed by Western blot (Figure1A). PrPC was detected in non-infected brain homogenates in equal amounts 40, 80, 120 and 150 days post infection (p.i.) (Figure1A, lanes 1, 3, 5, 7), while total PrP of scrapie-infected brain homogenates slightly increased during infection (Figure1A, lanes 9, 11, 13, 15), caused by the accumulation of PrPSc as demonstrated by proteinase K (PK)-digestion (Figure1A, lanes 10, 12, 14, 16).
To identify differentially regulated proteins in scrapie-infected mice, equal protein amounts of brain homogenates were separated by two-dimensional gel electrophoresis followed by Coomassie blue staining (Figure1B). Only two up-regulated protein spots were reproducibly detected in four individual mice infected by scrapie, which were then identified by mass spectrometry (Table1). Apolipoprotein E was found in three out of four investigated samples, while peroxiredoxin 6 (PRDX6) was identified with an overall sequence coverage of 71.4% in all four tested samples (Table1). Upregulation of apolipoprotein E has already been described in activated astrocytes in Alzheimer′s and prion disease and is considered as one of the strongest genetic risk factors in Alzheimer disease. Contrastingly, only marginal information is available on the expression of peroxiredoxins in prion disease. Peroxiredoxin protein was preferentially upregulated in astrocytes of prion-infected mouse brains, but it remained unknown whether all PRDX family members or a single isoform accumulated. Furthermore, PRDX6 protein expression in astrocytes has already been associated with Alzheimer disease where it might function as an antioxidant enzyme suggesting that PRDX6 might be involved in neurological diseases.
To investigate this in more detail, we followed PRDX6 expression during scrapie infection in mice and compared it with PRDX1-4 amounts by Western blot analyses (Figure1C). In contrast to non-infected mice (Figure1C, lane 1–4), the level of PRDX6 steadily increased within 150 days post infection (Figure1C, lane 5–8). This appears to be highly specific, since amounts of PRDX1-4 or PRDX2 did not change during infection (Figure1C, lane 5–8). Although we cannot exclude the possibility that PRDX1, 3 or 4 of the 2-Cys PRDX subgroup were slightly regulated, it is obvious that PRDX6 was strongly affected in scrapie-infected mice brains. Quantification of PRDX6 protein expression in brains of four individual mice at each time point after infection indicated that the observed effect was statistically significant and reproducibly observed in scrapie-infected mice (Figure1D).
Expression of peroxiredoxin 6 in PrP deficient and PrPC expressing neuronal precursor cells
To investigate PRDX6 expression in more detail, endogenous PRDX6 expression in PrP-deficient and PrPC-expressing cells was analyzed. The immortalized neuronal precursor cell line PrP0/0 ML derived from PrP0/0 ZrchI mice was stably transfected with wild type murine PrP (PrP A109). Western blots of these cell lines were performed for detection of PrP and PRDX6. As expected, PrP A109 cells expressed PrPC, whereas the PrP0/0 ML cells did not (Figure2A, upper panel). Corresponding to in vivo studies, PRDX6 expression was increased in PrP A109 cells (Figure2A, middle panel) while detection of β-actin indicated equal protein loading (Figure2A, lower panel). Analyzing mRNA synthesis of prdx6 and prnp, no significant alterations were observed indicating that PRDX6 protein expression was not transcriptionally dependent on PrP (Figure2B). To clarify whether PrP protein expression led to PRDX6 accumulation, PrP was downregulated using specific siRNAs and the amount of PRDX6 protein was analyzed by Western blotting. Interestingly, successful downregulation of PrP (Figure2C, lane 4) did not result in a detectable decrease in PRDX6 protein amount (Figure2C, lane 4). This finding was further supported by the inhibition of protein translation using cycloheximide. Twenty-four hours incubation of the cells with cycloheximide led to a drastic decrease in PrP expression, while leaving PRDX6 protein amounts unaffected (Figure2D, lanes 8–14). Although downregulation of PrP was not complete, these data imply that enhanced PrP expression does not induce PRDX6 expression and that PRXD6 is highly stable in neuronal cells.
PRDX6 induces upregulation of PrP in neuronal cells
Next, we aimed at the investigation of PRDX6 expression in scrapie-infected neuronal cells. Uninfected PrPC expressing N2a58# cells were compared to scrapie-infected N2a58/22L cells. Correspondingly to scrapie-infected mice, an upregulation of PRDX6 was observed in scrapie-infected N2a58/22L cells as shown by Western blot analysis (Figure3A, upper panel). Signal intensities from four independent experiments were quantified and expressed as fold PRXD6 expression normalized to β-actin expression (Figure3A, lower panel). Increased PRXD6 protein amounts were also detected by immunofluorescence analyses underlining enhanced protein occurrence in the cytoplasm, while PrP was mainly localized at the plasma membrane (Figure3B).
Since PRDX6 was upregulated in PrPC-expressing and PrPSc-infected cells, but not in cells in which PrPC expression was downregulated, we tested the hypothesis if PRDX6 is involved in PrP upregulation in turn. In N2a58# and N2a58/22L cells PRDX6 was successfully downregulated using PRDX6 specific siRNA that did not affect PRDX1-4 expression. Interestingly, PrPC in N2a58# cells was slightly decreased and PK-resistant PrPSc was strongly reduced in N2a58/22L upon siRNA treatment to inhibit PRXD6 expression (Figure3C). These data led to the suggestion that there is a functional connection between PrP and PRDX6 expression. Therefore, flag-tagged PRDX6 was overexpressed in N2a58# and N2a58/22L cells and the amount of PrPC and PrPSc was examined. Overexpression of PRDX6-flag had no influence on expression of PRDX1-4 (Figure3D). However, PRDX6-flag resulted in a slightly increased amount of PrPC in uninfected N2a58# (Figure3D, lanes 1–4) and subsequently to an obvious accumulation of PK-sensitive PrPC and PK-resistant PrPSc in infected N2a58/22L (Figure3D, lanes 5–8). PRDX6 exhibits a calcium-independent phospholipase A2 (iPLA2) activity. In N2a58/22L cells, iPLA2 activity was significantly increased compared to N2a58# cells. Importantly the difference was completely diminished after down-regulation of PRDX6 in both cell lines (Figure3E). In conclusion, these results suggest that the expression level and activity of PRDX6 might be involved in the control of the level of PrPC and subsequently PrPSc conversion.
In this study, enhanced amounts of PRDX6 was selectively identified in brains of prion-infected mice and neuronal cell lines concomitant with an increased amount of PrPC and consequently of PrPSc. This interaction appears very complex, since PrPC expression in PrP knock-out cells has also been observed to increase the amount of PRDX6 in turn, but downregulation of PrP did not alter PRDX6 appearance. This effect could be explained by the observation that the PRDX6 protein was more stable than PrP. Hence, the molecular basis for this phenomenon remains unknown, but might indicate a complex “tandem”-regulation of PRDX6 and PrP.
PRDX6 is a moonlighting protein containing peroxidase and PLA2 activities. Specific pharmacological inhibitors for cellular studies are not available, but it is tempting to speculate whether PRDX6 activities are involved in PrP regulation. In fact, data are accumulating that PLA2 contributes to prion diseases. Functionally, PLA2 is an important promoter of phospholipid metabolism and cleaves membrane phospholipids to produce arachidonic acid and lysophopholipids as major products. Under normal conditions, arachidonic acid is either re-incorporated into phospholipids, converted to inflammatory mediators in the brain or modulates neuronal functions. It has been demonstrated that PrPSc and the neurotoxic PrP106-126 prion peptide stimulated the N-methyl-D-aspartate (NMDA) receptor, which is accompanied by the release of arachidonic acid, suggesting an involvement of PLA2 in prion pathogenesis. This has been supported by neuronal cell culture studies showing that PLA2 is activated by glycosylphosphatidylinositols (GPIs) isolated from PrPC and PrPSc. Interestingly, treatment of CJD using the non-specific PLA2 inhibitor quinacrine resulted in an inhibition of PrPSc formation and reduced toxicity of PrP106-126. Together with our study, those data point to PRDX6 activities as new important players in the pathogenesis of prion diseases.
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We are grateful to Edgar Holznagel for valuable discussions and Kay-Martin Hanschmann for statistical analysis.
The authors declare that they have no competing interests.
WW: performed the experiments and wrote the manuscript. AR: conducted and interpreted mass spectrometry analysis. PH: performed the animal experiments. JL: participated in the design of the study and the interpretation of the results. SW: conceived of the study, and participated in design and coordination and wrote the manuscript. All authors read and approved the final manuscript.
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Wagner, W., Reuter, A., Hüller, P. et al. Peroxiredoxin 6 promotes upregulation of the prion protein (PrP) in neuronal cells of prion-infected mice. Cell Commun Signal 10, 38 (2012) doi:10.1186/1478-811X-10-38
- Peroxiredoxin 6
- Prion protein