Figure 1

Upregulation of PRDX6 in scrapie-infected mice. (A) C57Bl/6 mice were inoculated intracerebrally with PBS (not infected, lanes 1–8) or 10% brain homogenate of the prion strain 139A (scrapie-infected, lanes 9–16). 40, 80, 120 and 150 days post infection (p.i.) brain homogenates were prepared and incubated with 20 μg/ml proteinase K (+, PK) or left untreated (−) prior to Western blotting. PrP was detected using the 8H4 monoclonal antibody showing the typical migration pattern of PrP and PK-resistant PrP^res. β-actin was shown as a loading control. (B) 150 μg protein of brain homogenates were separated by two-dimensional gel electrophoresis followed by Coomassie blue staining. Enlarged sections indicate differentially expressed proteins (spots 1 and 2) observed in four tested homogenates. (C) 50 μg protein of brain homogenates were separated by SDS-PAGE followed by Western blot. PRDX6, PRDX1-4, PRDX2 and β-actin were detected using specific antibodies. (D) Quantification of protein expression was performed using four independent Western blots detecting PRDX6 (grey bars) and β-actin (black bars) in brain homogenates of four different mice, respectively. PRDX6 expression was by normalized to β-actin (**, p < 0.01, n.s., non-specific)