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Calcineurin stimulation by Cnb1p overproduction mitigates protein aggregation and α-synuclein toxicity in a yeast model of synucleinopathy

Cell Communication and Signaling volume 21, Article number: 220 (2023) Cite this article

Abstract

The calcium-responsive phosphatase, calcineurin, senses changes in Ca2+ concentrations in a calmodulin-dependent manner. Here we report that under non-stress conditions, inactivation of calcineurin signaling or deleting the calcineurin-dependent transcription factor CRZ1 triggered the formation of chaperone Hsp100p (Hsp104p)-associated protein aggregates in Saccharomyces cerevisiae. Furthermore, calcineurin inactivation aggravated α-Synuclein-related cytotoxicity. Conversely, elevated production of the calcineurin activator, Cnb1p, suppressed protein aggregation and cytotoxicity associated with the familial Parkinson’s disease-related mutant α-Synuclein A53T in a partly CRZ1-dependent manner. Activation of calcineurin boosted normal localization of both wild type and mutant α-synuclein to the plasma membrane, an intervention previously shown to mitigate α-synuclein toxicity in Parkinson’s disease models. The findings demonstrate that calcineurin signaling, and Ca2+ influx to the vacuole, limit protein quality control in non-stressed cells and may have implications for elucidating to which extent aberrant calcineurin signaling contributes to the progression of Parkinson’s disease(s) and other synucleinopathies.

Video Abstract

Background

The calcineurin phosphatase (CN), also called PP2B, is an evolutionary conserved protein belonging to the PPP3 class of serine-threonine phosphatases [1], which requires Ca2+ for its activity. Even minor changes in the exquisitely low cytosolic Ca2+ levels detected in unstimulated cells trigger distinct spatial and temporal cellular responses orchestrated and integrated by CN [1,2,3,4,5,6]. Apart from Ca2+ homeostasis, diverse pathways, including cell cycle control, pH homeostasis, and stress-activated transcriptional regulation are controlled by CN through its specific interaction with different substrates [6], relying on different substrate-binding docking motifs [3, 6,7,8].

CN is a heteromer encoded by CNA1 (or CNA2/CMP2) and the regulatory subunit encoded by CNB1. This complex acts downstream of the calmodulin effector molecule in response to Ca2+ binding. The association between calmodulin and CN releases the autoinhibitory domain from CN in the holoenzyme complex. The activated CN complex adjusts several fundamental cellular activities/features, including development, behavior, lifespan, and adaptive responses that are either dependent or independent of the downstream transcription factor CRZ1 [9,10,11,12,13,14,15,16]. CN-dependent Ca2+ homeostasis also involves multiple organelles and its dysregulation is known to cause problems also in protein homeostasis (proteostasis), including α-synuclein (α-Syn) proteinopathy related to Parkinson’s disease [17,18,19,20]. In addition, Habernig et al. [21], recently demonstrated that the cytotoxicity of α-Syn could be suppressed by administering extracellular Ca2+ and that this limited the oligomerization and toxicity of α-Syn by boosting lysosomal protease cathepsin D activity in a CN-dependent manner.

So far, the relationship between CN/Ca2+ signaling and Protein Quality Control (PQC) is not well understood. To elucidate the possible participation of CN and CN-dependent regulators, such as Ca2+ transporters/channels and downstream substrates of CN in PQC, we investigated to which extent such factors affected the generation and management of cellular protein aggregates in the yeast S. cerevisiae. We show that reduced CN signaling, through the deletion of the regulatory subunit CNB1, or deleting both the catalytic subunits (CNA1 and CMP2), during steady-state, non-stress conditions in Ca2+-replete medium triggers the formation of Hsp100p (Hsp104p)-associated protein aggregates and aggravates the cytotoxicity of both wild-type alpha-synuclein (α-Syn) and mutant α-Syn-A53T, associated with early-onset familial Parkinson’s disease. In contrast, enhanced Cnb1p production not only suppressed Hsp104p-associated protein aggregation but also supported growth in the presence of the hypertoxic α-Syn-A53T mutant allele. These effects were dependent, in part, on the transcription factor CRZ1 acting downstream of CN. The data suggest an intimate relationship between CN signaling and proper PQC and that CN/Ca2+ signaling limits proteostasis during normal steady-state growth under non-stress conditions and counteracts proteotoxicity associated with synucleinopathies.

Results

Altering calcineurin signaling in non-stressed cells affects protein aggregation

To study the consequences of calcineurin (CN) inhibition on protein quality control in yeast cells growing under non-stress conditions in Ca2+-replete media, we assessed the aggregation of cytosolic proteins using the yeast disaggregase Hsp104p fused to GFP as a reporter [22, 23]. In addition, we used the misfolding-prone allele of the yeast guanylate kinase enzyme, guk1-7p, tagged with mScarlett [24, 25] as a more direct reporter for misfolding [26]. Growing WT cells were treated with a 1 µg/ml concentration of the immunosuppressant drugs FK506 and CsA (Cyclosporin A) for 30 mins to inactivate CN. Cells treated with these drugs displayed increased protein aggregation as numerous Hsp104p-GFP foci formed in the cytosol in an enlarged fraction of cells (Fig. 1a). Next, we tested the effect of genetic deletions of individual subunits of the dimeric CN holoenzyme to assess the contribution of each subunit to proteostasis. For this, we created mutants defective in the catalytic subunits (cna1∆, cna2∆/cmp2∆) and the regulatory subunit (cnb1∆) carrying genomic Hsp104p-GFP. We found that deleting the redundant catalytic phosphatase subunits individually had little effect on aggregation while deleting both substantially increased the level of Hsp104p-GFP-associated aggregates (Fig. 1a). To further test whether reduced CN activity is encumbering PQC, we tested the Calmodulin allele cmd1-3, which encodes a mutant form of calmodulin that fails to bind Ca2+ and, therefore, cannot stimulate calcineurin activity [3, 6, 27, 28]. As expected, the strain showed much higher levels of cytosolic Hsp104p-GFP-associated protein aggregates than the WT strain (Fig. 1a). In addition, cells devoid of the regulatory subunit CNB1 displayed a more than three-fold increase in the fraction of cells containing Hsp104p-associated aggregates (Fig. 1a) as well as a six-fold increase in guk1-7p aggregates (Fig. 1b). Additionally, the guk1-7p and the Hsp104p associated aggregates were found to co-localize in cnb1∆ (Fig. 1b).

Fig. 1
figure 1

Effects of altered calcineurin activity on protein quality control. a Yeast cells expressing Hsp104p-GFP from its endogenous locus, treated as indicated with 1 µg/ml concentration of calcineurin inhibiting drugs FK506 and CsA for 30 min. Representative fluorescence microscopy images are displayed. The percentage of cells showing Hsp104p-GFP associated aggregates in WT, cna1∆, cna2∆, cnb1∆, and the double mutant cna1∆ cna2∆ are shown. b Representative images WT and cnb1∆ cells expressing Hsp104p-GFP (green) and guk1-7p-mScarlett (red). The cnb1∆ mutant accumulated more of Hsp104p-GFP (green) and guk1-7p-mScarlett associated aggregates of the reporter proteins than WT as quantified along with the microscopic images. c Quantification of the percent cells containing Hsp104p-GFP associated aggregates in WT cells and cells devoid of cnb1∆, hsp42∆, and cnb1∆ and hsp42∆ are shown. d Immunoblot analysis of Cnb1p-GFP levels regulated by the native or GPD promoter. Blot was probed with anti-GFP antibody and as a loading control a yeast anti-tubulin antibody. e Suppression of Hsp104p-GFP associated aggregates formation upon CNB1 overexpression. Total number of cells with aggregates shown for WT, GPD-CNB1, cmk2∆ and cnb1∆ cmk2∆ cells. f Immunoblot analysis of protein extracts from WT cells and the indicated deletion mutants to measure relative CN activation via the reporter plasmid pAMS366, expressing GFP under the control of 4X-CDRE [29]. Quantification of GFP vs. yeast tubulin protein levels as indicated (n = 3). g Quantification of Hsp104p-GFP associated aggregates indicated for various mutants in the young and aged population of WT, GPD-CNB1, cmk2∆ and cnb1∆ cmk2∆ cells. Quantitative data shown are averages of 3 independent samples and error bars indicate SD, where 200 cells were counted for each independent experiment. The scale bar represents 5 μm. *p < 0.05, **p < 0.005,***p < 0.0005, unpaired two-tailed t test

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It has been shown that the deposition of aberrant proteins into peripheral aggregates, namely IPODs and CytoQs, require the aggregase Hsp42p for their formation [24, 30,31,32] and we found that Hsp104p-associated aggregates formed upon defects in CN signaling (cnb1∆) required the presence of HSP42 (Fig. 1c). The data suggest that a constitutive level of CN signaling is required in cells neither starving for Ca2+ nor being subjected to Ca2+ overload, to avoid the formation of Hsp42p-dependent aggregates (IPODs and CytoQs) formation.

We next asked whether CN signaling is limiting for PQC in otherwise wild-type cells by expressing CNB1 under the control of the strong GPD promoter. Replacing the promoter resulted in a 3.5-fold increase in Cnb1p-GFP protein levels as detected by immunoblotting (Fig. 1d) and this overproduction markedly reduced Hsp104p-associated protein aggregation in wild-type cells (Fig. 1e). Another way to increase CN signaling is to delete CMK2, encoding a Calmodulin-dependent protein kinase acting as a negative regulator of CN signaling [33]. Deleting CMK2 resulted in a reduction in protein aggregation, albeit more modest than by Cnb1p overproduction (Fig. 1e). The reduction in aggregation achieved by deleting CMK2 required the presence of CNB1 (Fig. 1e) demonstrating that the effects of deleting this negative regulator act through CN signaling. To measure cellular calcineurin activity, we used a reporter plasmid containing 4X-CDRE-GFP (Calcineurin Dependent Response Element) [29] and observed a nearly 8-fold increase in CDRE-GFP levels in cmk2∆ and a more than 2.2-fold increased by overexpressing CNB1 (Fig. 1f). As expected, the mutants cnb1∆ and cmd1-3 caused a drastic decrease in CDRE-GFP levels (Fig. 1f) confirming that the deletions and genetic modifications made generated the expected outcome in terms of CN signaling. Our observations collectively indicate that constitutive CN signaling under normal, non-stress, growth conditions is required to avoid the formation of Hsp42p-dependent aggregates and that boosting such CN signaling by overproducing Cnb1p can further improve PQC.

To investigate a possible role of CN activity also on aggregate formation during ageing we isolated young and old cells of wild-type and CN-affected mutant strains, as previously described [34, 35]. As seen in Fig. 1g, aged cells lacking cnb1∆ accumulated more aggregates than the WT control but the difference between the strains was more evident in young cells. Similarly, constitutive overexpression of CNB1 suppressed the Hsp104p-GFP-associated aggregate formation in young cells but failed to do so in aged cells (Fig. 1g). In contrast, cells lacking cmk2∆ also suppressed age-associated aggregate formation (Fig. 1g). The data suggest that CN activation by Cnb1p overproduction and cmk2∆ leads to quantitatively different effects on CN activity that may cause qualitatively different outcomes on CN output functions and/or effects on somewhat different pathways (see Fig. 1f and below).

Cellular Ca2+ channels influence proteostasis differently

Basal [Ca2+] and CN signaling in the cell are maintained with the help of different Ca2+ regulating channels and pumps [36, 37]. Having established that some basal CN activity is critical for proper PQC we next determined which Ca2+ channel(s)/pump(s) are required in yeast cells to avoid protein aggregation under non-stress conditions. Specifically, Mid1p and Cch1p are two channels that work in parallel to provide Ca2+ from the cell exterior and we found that deleting both, as expected, resulted in an increased aggregation of cytosolic proteins, whereas individual deletions in the corresponding genes did not (Fig. 2a). It is unclear why mid1∆ resulted in a reduced aggregation but this could be due to a compensatory up-regulation of calcineurin activity and/or other not yet identified Ca2+ influx pathways contributing to Ca2+ homeostasis. The ER/Golgi-resident Ca2+/Mn2+ ATPase, Pmr1p, and the Golgi resident ATPase, Cod1p (Spf1p), contribute to Ca2+ homeostasis by sequestering Ca2+ into the lumen of ER/Golgi, an activity required for proper protein folding and transport through the secretory pathway [38,39,40]. We found that the deletion of the corresponding genes caused a reduction in cytosolic Hsp104p-GFP-associated aggregation (Fig. 2a). Cytosolic Ca2+ is regulated also by vacuolar pumps, including the Ca2+/H+ antiporter Vcx1p. Vcx1p is a low affinity/high capacity vacuolar antiporter in yeast cells [41]. Compared to wild-type cells, cells lacking VCX1 display a significantly lower release of Ca2+ from intracellular stores when permeabilized in a medium lacking Ca2+ [42]. The Ca2+ ATPase Pmc1p, and the channel protein Yvc1p, release excess Ca2+ from the vacuole [41, 43]. Among these three proteins, the absence of vacuolar pumps Pmc1p and Yvc1p had no noticeable effect on cytosolic protein aggregation (Fig. 2a). In contrast, the absence of Vcx1p reduced aggregation to levels lower than that observed in wild-type cells (Fig. 2a). We noticed that the cells lacking both vcx1∆ and pmc1∆ resulted in more cells with cytosolic protein aggregates, likely due to excess Ca2+ in the cytosol. A similar increase in aggregation was observed when WT cells were treated with high levels of external Ca2+ (50 mM CaCl2 (Fig. 2a)).

Fig. 2
figure 2

Influence of different Ca2+ channels on proteostasis. a Quantification of the percent cells containing Hsp104p-GFP associated aggregates in WT cells and various strains deficient in calcium regulatory channels as indicated. Results in WT cells treated with 50 mM CaCl2 for 30 min are also shown. b Immunoblot analysis of protein extracts from WT, cnb1Δ, crzΔ, and vcx1Δ cells expressing pAMS366 4X-CDRE-GFP [29]. Quantification of GFP vs. yeast tubulin protein levels is indicated (n = 3, ± SD). c Quantification of the percent cells containing Hsp104p-GFP associated aggregates in the young and aged population of WT and vcx1Δ cells. Data shown are the average of 3 independent ± SD. 200 cells from each independent experiments were counted and error bars indicate SD. *p < 0.05, **p < 0.005,***p < 0.0005, unpaired two-tailed t test

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Since the deletion vcx1∆ resulted in reduced aggregation, we investigated if this vacuolar Ca2+/H+ antiporter affected CN signaling by measuring relative 4XCDRE-GFP levels corresponding to CN-activation. Interestingly, genetic ablation of VCX1 resulted in marked activation of the CN-phosphatase (Fig. 2b). We also investigated the role of VCX1 during ageing and observed that the cells lacking VCX1 accumulated significantly fewer aggregates than the WT control irrespective of their age. Therefore, the data suggest that calcineurin activation achieved by depriving cells of VCX1 boosts proteostasis during ageing (Fig. 2c).

Calcineurin affects aggregate formation and aggregate clearance partly through the downstream target Crz1p

CN affects phosphorylation and activity of many downstream targets. Therefore, we tested whether the absence of any of these targets created a phenocopy of the absence of CN signaling (cnb1∆) to elucidate which CN-dependent pathway(s) are important for the proper management of protein aggregates. The CN-substrate proteins tested included Hph1p, Hph2p, Slm1p, Slm2p, Aly1p, Aly2p, and Crz1p. Among these, Aly1p and Slm1p/2p are involved in maintaining cytoskeleton integrity, membrane structure/function, and proper protein trafficking [44,45,46,47,48], whereas Hph1p/2p (Frt1p/2p) are ER-resident proteins involved in various stress responses, V-ATPase assembly, vacuole acidification, and Sec62p/63p-mediated translocation of specific proteins [49]. Lastly, CRZ1 is a Ca2+/CN-requiring transcription factor that, when dephosphorylated by CN, activates an array of genes by binding to a conserved CN-dependent response element (CDREs) on various stress response genes [50,51,52].

When testing mutants lacking any of these CN-dependent genes for aggregate formation in non–stress, Ca2+ replete, conditions we found that mutants lacking hph1∆, hph2∆ and crz1∆ displayed elevated formation of aggregates, of which cells lacking crz1∆ had the strongest increase (Fig. 3a). In contrast, aggregation in slm1∆ and aly2∆ cells was indistinguishable from that of wild-type cells, whereas slm2∆ or aly1∆ cells displayed a somewhat reduced aggregation (Fig. 3a). Increased aggregation in cells lacking hph1∆ or hph2∆ may be the result of failures in vacuolar pH control as such failures have been shown previously to boost protein aggregation in the cytosol [53].

Fig. 3
figure 3

Calcineurin affects aggregate formation and aggregate clearance partly through the downstream target CRZ1. a Quantification of total cells containing Hsp104p-GFP aggregates in WT cells and in mutants lacking known direct substrates of the CN phosphatase as indicated. b Quantification of total cells containing Hsp104p-GFP associated aggregates in WT cells and in mutants with altered CN activity having cnb1Δ or crz1Δ as indicated. c Quantification of the total cells containing Hsp104p-GFP associated aggregates in WT cells and in the vcx1Δ cells when having altered CN activity having cnb1Δ or crz1Δ as indicated. d and e Quantification of the total cells containing Hsp104p-GFP associated aggregates in WT cells and in various mutants after letting them recover at 30 °C for 60 min following a heat shock at 38℃ (60 min). Data shown are the average of 3 independent ± SD. 200 cells from each independent experiment were counted and error bars indicate SD. *p < 0.05, **p < 0.005,***p < 0.0005, unpaired two-tailed t test

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To test if the reduction in protein aggregation achieved by Cnb1p overproduction and a CMK2 deletion (Fig. 3b) acted through the downstream transcription factor CRZ1, we overproduced Cnb1p and deleted CMK2 in crz1∆ cells. Interestingly, we found that the CRZ1 deletion abolished the effect of the CMK2 deletion whereas Cnb1p overproduction could still reduce aggregation in crz1∆ cells (Fig. 3b and e). These results indicate that Cnb1p overproduction can affect PQC in a manner that is partly independent of CRZ1. In support of this notion, deleting CRZ1 in cnb1∆ cells resulted in an additive increase in protein aggregation (Fig. 3b). We were intrigued by the reduction of protein aggregation in cells lacking VCX1 (Fig. 2a), and we therefore also determined if this reduction was dependent on CN signaling by removing CNB1 or CRZ1 in vcx1∆ cells. Indeed, the reduced Hsp104p-GFP associated aggregation seen in vcx1∆ cells was partly counteracted by removing CNB1 or CRZ1 (Fig. 3c), suggesting that the effect of Vcx1p deficiency on proteostasis is, at least partly, dependent on CN signaling.

We next tested whether the alterations observed in aggregate formation correlated with an altered capacity of disaggregation in cells with altered CN activity and, if so, this was dependent on CRZ1. For this, exponentially growing, unperturbed cells were shifted to 38℃ for 60 min and next allowed to recover at 30℃ for 60 min. In all mutant and wild-type strains analyzed, 100% of cells contained Hsp104p-associated aggregates after 60 min at 38 °C and disaggregation was scored by assessing the fraction of cells having cleared themselves of aggregates following 60 min recovery at 30 °C. First, we found that activating CN signaling by deleting CMK2 accelerated aggregate clearance while Cnb1p-overproducing cells behaved like wild-type cells (Fig. 3d and e). Consistent with a role for CN in aggregate clearance, cnb1∆ cells displayed a markedly retarded disaggregation. Similar to the reduced aggregate formation in the absence of stress, accelerated disaggregation achieved by deleting CMK2 required the presence of CRZ1 (Fig. 3d). In further agreement with the involvement of Crz1p in disaggregation, crz1∆ cells, like cnb1∆ cells, displayed a markedly reduced ability to clear themselves of aggregates (Fig. 3d). Deleting CNB1 in cmk2∆ cells strongly suppressed the accelerated clearance of aggregates, suggesting, again, that the beneficial effects on aggregate management achieved by deleting CMK2 act through CN signaling. In contrast, the compromised disaggregation observed in crz1∆ cells was strongly suppressed by overproducing Cnb1p, supporting the idea that CNB1 overexpression and CMK2 deletion act on partially different pathways to reduce Hsp104p-associated protein aggregates and that these pathways to a different degree depend on CRZ1 (Fig. 3e).

Overproducing calcineurin B represses α-Syn A53T toxicity

As shown herein, CN signaling greatly impacts on the cell’s ability to deal with protein aggregates. To elucidate a potential relevance, if any, of these findings in disease models in yeast, we analyzed the toxicity of the human disease protein, alpha-synuclein (α-Syn) of the Parkinson’s disease, in yeast cells with altered CN signaling. A significant hallmark of this disease is the aggregation of α-Syn, which is normally localized to the plasma membrane where it acts as an inhibitor of phospholipase D [54, 55]. Previous studies have demonstrated that the α-Syn protein causes cytotoxicity by interfering with metal ion transport, cell membrane properties, protein quality control, autophagy, and trafficking [21, 56,57,58,59,60,61,62,63].

We tested the toxicity of wild-type α-Syn and α-SynA53T carrying a missense mutation associated with the familial form of Parkinson's generating postsynaptic deficits [64], using high copy number plasmids expressing GFP tagged and untagged α-Syn and α-Syn-A53T [54, 64,65,66,67,68,69,70,71,72,73,74]. The plasmids were propagated in the WT, cnb1∆, GPD-CNB1, and crz1∆ GPD-CNB1 strains. A drop test growth analysis revealed that α-Syn is not particularly toxic in a WT yeast strain, whereas the mutant α-Syn-A53T is (Fig. 4a). In contrast, cells lacking cnb1∆ were extremely sensitive to both α-Syn and α-Syn-A53T (Fig. 4a). Conversely, cells overexpressing CNB1 strongly suppressed the toxic effect of α-Syn-A53T (Fig. 4a). Additionally, we tested if suppression of α-Syn-A53T toxicity mediated by Cnb1p overproduction required CRZ1. As shown, resistance towards α-Syn-A53T achieved by overproducing Cnb1p was partly dependent on the presence of CRZ1 (compare Fig. 4a, right panel with Fig. 4b, right panel).

Fig. 4
figure 4

CN stimulation by calcineurin B overproduction represses α-Syn-A53T toxicity. a and b Representative drop test of from the indicated strains carrying α-Syn or α-Syn-A53T on a high copy number plasmid. A five-fold dilution for the control and indicated strains were spotted onto plates containing SD − Ura, and imaged after 72 h. c Yeast cells expressing GFP-fusions of α-Syn or α-Syn-A53T were visualized by fluorescence microscopy (right panel). α-Syn-A53T-GFP was localized in a puncta at the cell membrane and some scattered aggregates in the cytoplasm in wild-type cells (strain BY4741). In the cells overexpressing CNB1, α-Syn-A53T-GFP was evenly localized at the cell membrane only. In the crz1∆ cells overexpressing CNB1, α-Syn-A53T-GFP displayed an even distribution at the cell membrane and also formed large scattered inclusions on the cytoplasmic face of the cell membrane. Fraction of the cells plotted for the indicated strains expressing α-Syn or α-Syn-A53T-GFP (green) aggregates localizing to the cell membrane or dislocating to cytosolic puncta. d Percent fraction of the mutant strains indicated displaying nuclear localization of the transcription factor Crz1p-GFP in the strains expressing empty vector, α-Syn or α-Syn-A53T. Localization data shown in (c and d) are an average of 5 independent experiments ± SD. 200 cells from each independent experiment was counted and SD is depicted by the error bars. The scale bar represents 2 μm. *p < 0.05, **p < 0.005,***p < 0.0005, unpaired two-tailed t test

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Previously, several studies utilizing yeast models of proteinopathy have shown that α-Syn predominantly localizes to the plasma membrane [54, 55, 75]. Primarily, α-Syn could be found in two states: a soluble unfolded monomeric form and a membrane-associated multimeric form, whereas the pathological state of the protein is either an oligomeric form and/or amyloid fibrils [76]. We found that WT cells expressing α-Syn-A53T-GFP displayed aggregated membrane-associated puncta along with some cytoplasmic aggregates whereas this localization was somewhat disrupted in the cells harboring cnb1∆ (Fig. 4c). Interestingly, cells overexpressing CNB1 displayed an enhanced localization of α-Syn-A53T-GFP to the cytosolic membrane and a concomitant decrease in its aggregation (Fig. 4c); phenotypes that were dependent on CRZ1 (Fig. 4c). Following up on these results, we found that Cnb1p overproduction in cells carrying either of the α-Syn proteins boosted the nuclear localization of Crz1p-GFP [77] whereas this pattern was disrupted in cells lacking CNB1 (Fig. 4d). Thus, the wild-type and A53T α-Syn alleles activate CN signaling to different extents in a manner influencing the degree of toxicity. Notably, the activation of CN signaling and Crz1p localization to the nucleus in the presence of α-Syn proteins was, in wild type cells, only able to overcome the toxicity of the less toxic α-Syn allele, whereas ectopic overproduction of Cnb1p overcame the toxicity of both alleles.

Discussion

In this study, we found that the CN phosphatase and its regulator, calcineurin B, prevents Hsp42p-dependent, Hsp104p-associated, aggregate formation in non-stressed, Ca2+-replete cells (Fig. 1). The fact that the aggregates formed in cells with compromised CN signalling required HSP42 for their formation indicates that they are sorted spatially to the IPOD and Q-body (or Stress Foci) deposits/quality control compartments [24, 30,31,32]. We show also that activation of CN signalling, either by overproducing Cnb1p or deleting CMK2, a negative regulator of CN activity [33], causes a dramatic reduction in the cells containing Hsp104p-associated and guanylate kinase (guk1-7p)-associated aggregates, suggesting that CN signalling is limiting for protein quality control in non-stressed yeast cells. Similarly, suppression of aggregate formation and activation of CN signalling could be achieved by deleting the vacuolar Ca2+ influx pump VCX1 suggesting that vacuolar Ca2+ storage is limiting CN activity, which, in turn, causes protein aggregation in the cytosol of non-stressed cells (Fig. 2). We, therefore, conclude that the balance between vacuolar Ca2+ storage capacity vs. free cytosolic [Ca2+] plays a pivotal role in determining the overall efficiency of the PQC machinery in non-stressed cells under calcium-replete conditions through the CN-dependent regulatory pathway (Fig. 5).

Fig. 5
figure 5

An overview of Calcineurin dependent bottlenecks regulating proteostasis in unstressed yeast. Activated calcineurin dephosphorylates the downstream transcription factor Crz1p stimulating the expression of target genes related to calcium homeostasis. Among these are channels that transport calcium into the ER, the vacuole or outside to restore homeostasis. Under calcium-replete conditions, normal calcineurin activity and balanced proteostasis are maintained by plasma membrane (Cch1p and Mid1p), vacuolar (Vcx1p and Pmc1p) and ER pumps (Pmr1p). The calmodulin-dependent kinase CMK2 inhibits Crz1p activity by phosphorylating multiple amino acid residues. Upon stimulation by calcium, mutational perturbation of calcium signaling or aberrant proteostasis, calcium accumulates in the cytosol along with Hsp42p-dependent protein aggregates (PA). Calcineurin phosphatase stimulation, via an unknown effector (X), α-synuclein (α-Syn) toxicity and protein misfolding (pM) for native protein (pN)the latter of which suppresses protein aggregation. Where, enforced calcineurin activation by Cnb1p overproduction boosts proteostasis and suppresses α-Syn toxicity, suggesting that calcineurin activity is limiting for proteostasis under normal conditions. The T-shaped bars represent inhibition

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Deleting a well-studied CN client transcription factor, CRZ1, resulted in increased protein aggregation (Fig. 3a), which could not be repressed by deleting CMK2. Thus, the reduction in protein aggregation achieved by deleting CMK2 in wild-type cells appears to act through CRZ1. In addition, accelerated clearance of protein aggregates achieved by deleting CMK2 similarly required the CRZ1 regulator (Fig. 3d). However, activation of CN signalling through overproduction of Cnb1p required CRZ1 neither to reduce protein aggregation nor to accelerate aggregate clearance, suggesting that CNB1 overexpression could function independently of CRZ1 (Fig. 3b and e). Thus, it appears that signalling through CNB1 functions in both CRZ1-dependent and -independent manners in cellular PQC.

The reduced aggregate formation observed in cells lacking the vacuolar influx pump VCX1 was suppressed when CNB1 was deleted (Fig. 3c), demonstrating that vacuolar Ca2+ influx is affecting PQC, at least partly, through CN signaling. It is possible also that the beneficial effects on PQC achieved by deleting VCX1 acts through a post-transcriptional upregulation of another vacuolar Ca2+ channel, PMC1 [33, 50, 78, 79]. Indeed, the reduced aggregation in vcx1∆ cells were completely abolished when PMC1 was concurrently deleted (Fig. 2a). Such an enhanced aggregation could be due to Ca2+ overload in the cytoplasm resulting from the lack of vacuolar Ca2+ sequestration when both these vacuolar channels are absent, similar to a situation when cells were challenged with a high concentration of extracellular Ca2+ (Fig. 2a) (see also [42]).

S. cerevisiae has been adopted as a model for studying cellular and molecular aspects of Parkinson’s disease-associated α-Syn misfolding, aggregation, and toxicity. A distinct hallmark of this protein is that it forms inclusions bearing α-Syn protein monomers [54, 55] and several studies have shown that yeast cells overexpressing α-Syn display higher cytosolic Ca2+ levels [18, 21, 80, 81]. Therefore, we tested WT α-Syn and the missense mutation A53T associated with the familial form of Parkinson’s disease expressed from a high copy number plasmid [54, 82] in various yeast mutants known to affect calcium homeostasis and signalling. Interestingly, only the cells overexpressing CNB1 strongly suppressed the toxic effects of both α-Syn and its variant A53T. Conversely, yeast cells harbouring cnb1∆ displayed enhanced sensitivity to both α-Syn and its variant A53T. Suppression of α-Syn and A53T α-Syn toxicity achieved by overexpressing CNB1 correlated with a normal membrane localization of the disease protein and, simultaneously, a reduced cytosolic aggregation (Fig. 4c). This indicates that in a yeast model of Parkinson’s disease and overall proteostasis, enhanced CN signalling by Cnb1p overproduction affects properties of α-Syn that have previously been linked to the disease-associated pathological effects (Fig. 5). Our findings suggest that elucidating to which extent aberrant CN signaling is contributing to the progression of Parkinson’s disease, and perhaps other proteinopathies, may be warranted.

Materials and methods

Yeast strain construction

The various knockout mutants used were constructed in BY4741 by integrating the gene::HphNT1 hygromycin cassette amplified using the pYM20 integrative module [83] enlisted in Table 1. The GPD promoter was amplified from the pYM-N15 plasmid [83] and incorporated upstream of the CNB1 ORF in the WT BY4741 and in CNB1-GFP. The C-terminal EGFP-HIS3-MX cassette was amplified from the pYM-28 plasmid [83] and was incorporated in frame with the ORF of CRZ1 and the HSP104 respectively. For removing GFP from α-Syn-GFP and A53T-GFP variants ClaI-XhoI restriction digestion was performed and the linearized pRS426 GPD harboring the α-Syn and A53T variants of α-Syn on the plasmids were re-ligated.

Table 1 S. cerevisiae strains used in this study
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Protein aggregation visualization and aggregate clearance analysis

As previously described [24], cells were grown in medium supplemented with 2% glucose at permissive temperature (30 °C/22 °C) until the mid-exponential phase (OD600 0.5). Where indicated, Cyclosporin A from (Sigma) and FK506 (LC Laboratories, Woburn MA) were used at 1 μg/mL and CaCl2 (Sigma-Aldrich, St. Louis, MO) was used at 50 mM). Protein aggregation was also induced by shifting the cells to 38 °C for 60 min or by allowing cells to age replicatively at 30 °C. Cells were fixed in 3.7% final concentration formaldehyde and washed with PBS. Z-stack images were acquired using a conventional fluorescence microscope; a Zeiss Axio Observer Z1 inverted microscope equipped with an Axiocam 506 camera and a Plan-Apochromat 100x/1.40 NA Oil DIC M27 objective. Images were quantified by manually counting the fraction of cells containing aggregates in the ImageJ software. All the data are based on the average of at least three individual experiments, with error bars representing SD.

Statistical analysis

Data from quantifications are presented as the average of ≥ 3 biological experiments ± SEM unless indicated otherwise. Aggregate quantifications of cells are based on counting 200 or more cells per strain at each condition for three biological replicates. Data analysis and visualization were performed with GraphPad Prism 8.3.1. In figures, asterisks denote statistical significance determined by the indicated statistical test with ns p > 0.05, *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p < 0.0001.

Co-localization analysis

Cells with the indicated fluorescent protein reporters were grown in either YPD or SCD as indicated supplemented with 2% glucose at a permissive temperature (30˚C) until the mid-exponential phase (OD600 0.5).

Gel electrophoresis and immunoblotting

Cells were harvested in exponential phase at an OD600 of 0.8, as previously described [29]. A total of 6.0 OD600 cells were then lysed in 0.2 M NaOH, supplemented by complete™ Protease Inhibitor Cocktail (Roche) and Halt™ Phosphatase Inhibitor Cocktail (Fischer scientific), incubated for 20 min on ice, pelleted, resuspended in 50–70 μl sample buffer (1 × Laemmli buffer (80 mM Tris–HCL pH 6.8, 10 mM EDTA pH 8.0, 8% SDS, 20% Glycerol, 0.004% Bromophenol blue), 8 M Urea, 2.5% β-Mercaptoethanol) and heated for 5.0 min at 90 °C. About 15 μl of the protein sample was loaded per lane of a 12% Criterion TGX Precast Midi Protein Gel (Bio-Rad, Hercules, CA, USA), blotted onto Trans-Blot Turbo Midi Nitrocellulose Transfer Packs (Bio-Rad, Hercules, CA, USA), and probed overnight with primary antibodies: Membranes were probed with anti-GFP (ab6556; Abcam). As secondary antibodies, goat anti-mouse IRDye 800CW and goat anti-rabbit IRDye 680 (LI-COR; 1/20000 dilution) were used. Membranes were scanned using the LI-COR Odyssey Infrared scanner. Images were then quantified by the ImageJ software.

Drop tests on agar plates

Strains with the indicated plasmids were grown in the indicated media (YPD or SCD) supplemented with amino acids along with 2% glucose at the permissive temperature (30 °C/22 °C) until the mid-exponential phase (OD600 0.7). The culture ODs were adjusted to 0.5 on YPD and SCD (OD600 ~ 1.0) for serial dilutions and then spotted on agar plates for colony formation. The cell suspensions were spotted onto the respective drop-out media plates and incubated at the indicated temperatures for 2–3 days.

Old cell isolation

Old cells were obtained using the magnetic beads biotin-streptavidin system according to established protocols [35]. Cells were grown to exponential phase at 30 °C, approximately 3 × 107 cells were harvested, washed in PBS and then labelled with EZ link Sulfo-NHS-LC biotin (Thermo Scientific) at a final concentration of 0.5 mg/mL in 1 ml. Excess biotin was washed away, and cells were resuspended in 1L growth medium and cultured for about 15 h overnight at 30 °C, using an orbital shaker (180 rpm). When the cultures had reached an OD600 of about 0.5, cells were washed with PBS and incubated with 0.05 mg/ml MagnaBind streptavidin beads (Thermo Scientific). Biotin-labelled cells were then isolated using a magnetic sorter and continuous washes with PBS containing 0.5% glucose. Cells were resuspended in a 7 ml growth medium and used directly for subsequent experiments. The median age of the old cells was determined by counting bud scars in z-stack images upon staining cells with 10 µg/mL Wheat Germ Agglutinin (WGA, Thermo Scientific).

Determination of calcineurin activity

Calcineurin activity was determined using a reporter plasmid pAMS366- 4xCDRE-GFP described in [29]. Briefly, at about an OD600 of 2.0, yeast cells were harvested and then lysed in 0.2 M NaOH supplemented by complete™ Protease Inhibitor Cocktail (Roche) and Halt™ Phosphatase Inhibitor Cocktail (Fischer scientific), incubated for 20 min on ice, pelleted, resuspended in 50–70 μl sample buffer (1 × Laemmli buffer (80 mM Tris–HCL pH 6.8, 10 mM EDTA pH 8.0, 8% SDS, 20% Glycerol, 0.004% Bromophenol blue), 8 M Urea, 2.5% β-Mercaptoethanol), and heated for 5.0 min at 90 °C. The lysates were snap-chilled and spun for one minute and about 15 μl of the sample was loaded on the SDS-PAGE.

Availability of data and materials

All data are contained within this article.

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Acknowledgements

We would like to thank Lukasz Syga for providing the guk1-7 mScarlett integration template, Sabrina Buttner for mutant cna1∆cna2∆, Martha Cyert (Stanford University, USA) for kindly sharing pOM4-CRZ1-3XGFP plasmid, and Tiago Fleming Outeiro for α-Syn-GFP and A53T-GFP bearing plasmids.

Funding

Open access funding provided by University of Gothenburg. This work was supported by the Swedish Research Council Natural Sciences to T.N and M. M and Knut and Alice Wallenberg Foundation to T. N (Project numbers KAW Research Project 2019.0091, KAW Scholar 2019.0280, VR 2019–03937 and VR 2020–05422 to MM.

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Authors and Affiliations

  1. Institute for Biomedicine, Sahlgrenska Academy, Centre for Ageing and Health – AgeCap, University of Gothenburg, Gothenburg, 405 30, Sweden

    Srishti Chawla, Kara L. Schneider, Navinder Kumar & Thomas Nystrom

  2. Department of Life Sciences, Chalmers University of Technology, Gothenburg, Sweden

    Srishti Chawla & Mikael Molin

  3. Center for Bionics and Pain Research, Sahlgrenska University Hospital, Mölndal, 431 30, Sweden

    Doryaneh Ahmadpour

  4. Max Planck Institute for Biology of Ageing, Cologne, 50931, Germany

    Arthur Fischbach

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  1. Srishti Chawla
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Contributions

SC and TN designed the experiments. SC, NK, DA, KLS and AF performed the experiments. SC, NK, DA, KLS, MM and TN analysed the experiments. SC and TN wrote the manuscript with inputs from all the authors. SC,MM and TN edited the manuscript with inputs from all the authors.

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Correspondence to Srishti Chawla or Thomas Nystrom.

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Chawla, S., Ahmadpour, D., Schneider, K.L. et al. Calcineurin stimulation by Cnb1p overproduction mitigates protein aggregation and α-synuclein toxicity in a yeast model of synucleinopathy. Cell Commun Signal 21, 220 (2023). https://doi.org/10.1186/s12964-023-01242-w

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  • DOI: https://doi.org/10.1186/s12964-023-01242-w

Keywords

Cell Communication and Signaling

ISSN: 1478-811X