Fig. 5
From: Melanoma stimulates the proteolytic activity of HaCaT keratinocytes
Identification of gelatinases present in CAKs media and MMP14 activity. Zymography analysis of media collected from CAKs (A). Western Blotting analysis of MMP9 level in CAKs media (B). MMP14 activity assay performed on cell lysates (C). Cells were cultured in the presence of melanoma on Transwell inserts (INS) or with melanoma-conditioned media (CM). Control (CTRL) constitutes of cells cultured in kDMEM: mDMEM (1:1 ratio) media analogously to tested cells. In the case of Western Blotting analysis results were normalized to Ponceau S staining. The mean of at least three biological repetitions ± SD is shown. Asterisks indicate statistically significant differences between control cells and CAKs or between different types of CAKs. The significance level was set at p ≤ 0.05 (*), p ≤ 0.01 (**), and p ≤ 0.001 (***)