Fig. 3
From: The Janus-faced role of TRPM2-S in retroperitoneal liposarcoma via increasing ROS levels
TRPM2-S promotes proliferation via activating ERK and AKT. a Western blotting indicated TRPM2-S expression in pcDNA3.1-TRPM2-S-Flag cells was obviously higher than in empty vector RPLS cell lines. b TRPM2-S inhibited the intracellular flow of Ca2+. c Growth curves of live-cell imaging showed TRPM2-S-expressing 94T778 and SW872 cells have a higher proliferative capacity than negative control cells. d Representative images of EdU assays (left panels). EdU showed that a significant proliferation rate was observed in TRPM2-S-expressing RPLS cell lines (right panels). e Western blotting revealed that p-Akt, p-GSK3β, β-Catenin, and p-ERK were upregulated, whereas FOXO3a and PTEN were downregulated in TRPM2-S-expressing cells compared with control cells