Fig. 5
From: Presenilin1 inhibits glioblastoma cell invasiveness via promoting Sortilin cleavage
Sortilin is substrate for Presenilin1 and mediates the suppression of Presenilin1 on β-catenin in glioblastoma cell. A, B Western blot analysis the expression change of Sortilin inU87 and U251 cells transfected with Lv-PS1, Sh-PS1 or control. C, D Western-blot assays to investigate the expression levels of full length (FL-) Sortilin, cleavage heavy (CH-) Sortilin and cleavage light (CL-) Sortilin after down- or up-expression ofPresenilin1 in U87 and U251 cell. E, F Western-blot assays to show the expression ofp45-β-catenin, p33/37/41-β-catenin, stabilized β-catenin when down-regulation of Presenilin1 or combined with down-regulation of Sortilin in U87 and U251 cells. G, H Western-blot assays to show the expression ofp45-β-catenin, p33/37/41-β-catenin, stabilized β-catenin when up-regulation of Presenilin1 or combined with up-regulation of Sortilin in U87 and U251 cell. **p < 0.01, *p < 0.05