Fig. 5

SIRT1 inhibition inhibits the protection induced by mTOR/PARP-1 knockdown against light injury. a 661 W cells were pretreated with 150 μM EX527/vehicle for 6 h and cultured with fresh medium under normal conditions for 72 h. Cell mortality was then quantified by PI/Hoechst staining. b, c Cells with mTOR/PARP-1 knockdowns were pretreated with 150 μM EX527/vehicle for 6 h and exposed to 1500 lx light while culturing with fresh medium for 72 h. Cell mortality was quantified by PI/Hoechst staining. mTOR KD: mTOR knockdown; PARP-1 KD: PARP-1 knockdown; Lt: light exposure for 72 h; EX527: SIRT1 inhibitor. Scale bar = 100 μm. All experiments were repeated in triplicate, and the results are shown as the means ± SEM (**: P < 0.01, ns: not statistically significant)