Fig. 4
From: mTOR may interact with PARP-1 to regulate visible light-induced parthanatos in photoreceptors
SIRT1 inhibition blocks crosstalk between mTOR and PARP-1 signals under light exposure conditions. a-c Cell lysate samples were analyzed by Western blot with β-ACTIN used as an internal control. Scramble: cells transfected with scrambled shRNA were used as a negative control; mTOR KD: mTOR knockdown; PARP-1 KD: PARP-1 knockdown; Lt: 1500 lx light exposure for 72 h. EX527: a SIRT1 inhibitor. The target proteins levels were quantitatively analyzed relative to β-ACTIN. All experiments were repeated in triplicate, and the results are shown as the means ± SEM (*: P < 0.05, **: P < 0.01, ***: P < 0.001)