Fig. 5
From: Androgen promotes differentiation of PLZF+ spermatogonia pool via indirect regulatory pattern
WT1 is an intermediate molecule to transmit androgen signal to downstream target β1-integrin. The representative view of WT1 binding sites (visualized by Integrative Genomics Viewer [http://software.broadinstitute.org/software/igv/]) at β1-integrin promoter region (a). ChIP-qPCR validates the binding of WT1 in the promoter region of β1-integrin: fold enrichment by antibodies against WT1 relative to control IgG was presented as mean value ± SD of two replicates (b). Knockdown of WT1 in Sertoli cells decreased β1-integrin expression, 1, Wt-1 siRNA; 2, scambled siRNA; 3, untransfected control, n = 5 (c), and transfection of Wt1 cDNA enhanced β1-integrin expression, n = 3 (d). Androgen down-regulated expression of WT1 and β1-integrin in co-culture system, n = 4 (e). Knockdown of β1-integrin in the co-culture system suppressed expression level of PLZF, 1, Itgb1 siRNA; 2, scambled siRNA; 3, untransfected control n = 3 (f). An illustration hypothesizes the potential signal pathway from AR to β1-integrin in Sertoli cells (g)