Fig. 3

The changes in metabolic enzymes contribute to the low levels of pyruvate. a Cells were treated with 1 μM ML265 for 24 h and subjected to western blot. b PKM2 overexpressed cells and their counterparts were subjected to western blot. c The oxygen consumption rates (OCR) of PKM2-overexpressed cells and control cells were detected at different time points. OCR under oligomycin, carbonyl cyanide 4-(trifluoromethoxy) phenylhydrazone (FCCP), and antimycin A/rotenone treatments, respectively. d Cells were treated with 10 μM Gossypol-Acetic Acid (GAA) for 24 h and subjected to western blot. e LDHA knockdown cells and their counterparts were subjected to western blot. f The OCR of LDHA knockdown cells and control cells were detected at different time points. g Cells were treated with 1 μM ML265 and 10 μM GAA for 24 h, then subjected to western blot. h Cells were treated with 1 μM ML265 and 10 μM GAA for 24 h, the concentrations of the metabolites in GC cells were measured by Mass spectrometry (MS) and normalized to their protein level. i The OCR of cells were detected at different time points after they treated with 1 μM ML265 and 10 μM GAA for 24 h. Data represent the Mean ± SEM, n ≥ 3. *p < 0.05, **p < 0.01, NS not significant