Perspective on nanochannels as cellular mediators in different disease conditions
Cell Communication and Signaling volume 16, Article number: 76 (2018)
Tunnelling nanotubes (TNTs), also known as membrane nanochannels, are actin-based structures that facilitate cytoplasmic connections for rapid intercellular transfer of signals, organelles and membrane components. These dynamic TNTs can form de novo in animal cells and establish complex intercellular networks between distant cells up to 150 μm apart. Within the last decade, TNTs have been discovered in different cell types including tumor cells, macrophages, monocytes, endothelial cells and T cells. It has also been further elucidated that these nanotubes play a vital role in diseased conditions such as cancer, where TNT formation occurs at a higher pace and is used for rapid intercellular modulation of chemo-resistance. Viruses such as HIV, HSV and prions also hijack the existing TNT connections between host cells for rapid transmission and evasion of the host immune responses. The following review aims to describe the heterogeneity of TNTs, their role in different tissues and disease conditions in order to enhance our understanding on how these nanotubes can be used as a target for therapies.
Tunnelling nanotubes (TNTs) are intercellular cytoplasmic channels that enable direct physical interactions, facilitating the transport of cellular cargo between a range of different cell types. These have been referred to in recent literature by a number of names including “nanochannels”, “cytoplasmic extensions”, “intercellular bridges”, “nanotubular highways” or “epithelial bridges”. These are generally fine, long, non-adherent and are composed of F-actin-based extensions and function to promote intercellular cargo transfer between proximal and distant cells . Generally, there are two important types of nanotube: close-ended and open-ended tunnelling nanotube. Junctional borders are present in close ended cell-to-cell bridges i.e., innate nanotubes which connect T-cells and the MLV-induces cellular bridges. Through these cellular bridge cargo has to cross the plasma membrane boundary. According to this finding HIV-1 fusion inhibitor T20 can block the receptor-dependent transmission of HIV-1 Gag . On the other hand, tunneling nanotubes (TNTs) are product of de novo formation of membranous nanotubes between cells which helps in cell-to-cell communication. TNTs are F-actin rich structures which is involved in mediating membrane continuity. It is also shown to be involved in intercellular transport of various cellular components . TNTs are involved in several diseases such as cancer, viral infection, Parkinson’s disease [4,5,6].
While similar actin-based extensions such as lamellipodia, invadopodia and filopodia exist, they differ in their properties such as diameter, adherence and cell-cell cytoplasmic interaction . Furthermore, TNTs have been observed to be not affected by trypsin digestion and are devoid of microtubules . The walls of the nanotubes are continuous with the plasma membrane and are hence composed of the same bilipid membrane layer and the lumen of the nanotube connects the cytoplasm of the connecting cells.
Studies have demonstrated the transfer of everything from prions [8, 9], retroviruses [10, 11], apoptotic signals , calcium signals  to full length RNA, proteins and organelles  through these nanochannels. Data from studies in in vitro and ex vivo experiments suggest that uninfected healthy cells have a minimal number of nanotube connections with adjacent cells, but they were observed to be significantly higher in disease conditions such as cancer, viral infections and prion-associated diseases, among others [8, 9, 15]. There are several ways such as cell-cell communication, organelle exchange, cargo delivery and tissue repair in which TNTs play important role. Using pharmacological modulator several pathological pathways can be targeted for disease management. Understanding morphology and structural organization of TNTs can help us in finding the key molecular target for treating disease. Usually TNTs are involved in immune responses. Several study suggest the localization and transfer of class 1 MHC through TNTs. To escape the immune cells, viruses usually spread from cell-to-cell through TNTs. It has been observed that Human T-cell Leukemia virus migrates from infected to uninfected cells through TNTs and avoid recognition by immune cells. It has been found that HTLV-1 protein p8 increases the viral transmission. Treatment of MT-2 cells by cytarabine reduced TNTs formation induced by HTLV-1 p8 protein. Targeting these nanotubes can aid in treating disease caused by this virus. TNTs are also found to be involved in lung adenocarcinoma tumor cells [1, 16]. Increased number of TNT formation has been observed in co-culture of rat primary astrocytes and C6 glioma cells due to oxidative stress . There are several diseases associated with oxidative stress such as diabetes, Parkinson’s and cancer. In stress condition TNTs can transfer mitochondria which serves as the rescue mechanism .
This review highlights the functional role of TNTs in cancer development, progression and metastasis, along with its role in viral infection.
TNTs facilitate cell-cell communication in cancer cells
These nanotubes play a vital role in the intercellular communication in cancer cells and have been characterized in multiple cancer subtypes as a facilitator of cancer cell to cancer cell communication, as well as cancer cell to tumor matrix communication. It has been postulated that this intercellular communication with the tumor matrix may play a direct role in the progression and development of solid tumors . TNTs have been detected to play a role in multiple cancer types including lung adenocarcinomas , prostate cancer [7, 14], colon cancer  and glioblastoma .
Nanochannels between cancer cells were first described in 2004 paper using imaging techniques such as SEM (scanning electron microscopy) and TEM (transmission electron microscopy) where organelle transport was evidenced in HEK, NRK and PC12 cell lines . A study in 2012 on the role of nanochannels using immunofluorescence imaging and confocal microscopy in pleural mesothelioma cells provided evidence that suggested direct bidirectional transfer of not only chemical messengers, but also proteins and organelles such as mitochondria and Golgi vesicles . In this study, TNTs formed by migrating cancer cells were observed in time-lapse confocal imaging and were found to extend nanotubes during migration to fill the gaps created during a scratch wound assay which indicates a role of nanochannels in cancer invasion . This is supported by the discovery of fascin, an actin filament bundling protein that has been identified as a component of nanotubes and has been previously implicated in the initiation of metastases .
One study identified these nanochannels as facilitators of cross-talk between tumor cells and endothelial cells during the process of metastasis. The cancer cells were observed to regulate the endothelium through the transfer of microRNAs, which was evidenced by a higher expression of pathogenic endothelium markers that was reversed upon pharmacological inhibition of nanochannel formation . The research suggested that cancer cells are able to dynamically modulate healthy cells through horizontal communication using nanochannel conduits. TNTs have also been studied in prostate cancer cells where it was demonstrated through immunofluorescence imaging that alpha-tubulin also acts as a microtubule component in nanotubes that are more than 1 μ in diameter . Microtubule is one of the structure which has been seen in TNTs and is suggested by several research group. TNTs are transient structures which last from few minutes to hours. Study done by Differential interference contrast (DIC) imaging found that hippocampal neurons from E18 rats make TNTs in which these transient structures have microtubules but lack F-actin . Davis et al. demonstrated that macrophages form different types of TNTs which contain microtubules and F-actin. These TNTs are involved in large material transport such as lysosomes and mitochondria . Here, we believe that component of TNTs are based on cell type and the function it performs during cell-to-cell communication . However, there is need for identifying exact component of cell-specific TNTs.
A study on nanotubular membrane extensions in colon cancer concluded that these TNTs are extremely sensitive to mechanical stress, but also very dynamic in nature. They have the ability to retract and form channels again through extension of filopodia-like structures that make contact with the neighbouring cells . The elastic properties of cancerous TNTs were further assessed on glioblastoma cells where existing nanotubes between cancer cells were observed under lateral tension and force. It was found that these nanochannels are highly elastic, and were observed to bifurcate instead of breaking when stress was applied at one point indicating much higher resilience than expected .
Studies have also demonstrated that malignancy can be induced by cancer cells in neighbouring healthy cells using nanochannels through the transfer of microRNAs between them. TNTs were observed to transport oncogenic microRNAs which have been identified to be upregulated in cancer, specifically miR-19a in osteosarcoma and miR-199 in ovarian cancer, to non-malignant cells . Exosomes, another form of cell-cell communication, have been observe to co-localise near existing nanochannels and stimulate an increased rate of TNT formation .
The propagation of chemo-resistance through nanotubes between cancer cells is a compelling biological phenomenon that has been observed in few studies. This hypothesis of chemo-resistance development by cancer cells through intercellular transfer was first put forward in 1991 in Frankfurt et al., but TNTs as conduits were not implicated until much later . The formation of nanochannel structures between cancer cells have been widely studied and seems to rely on the mTOR pathway, evidenced through the marked decrease in TNT formation upon suppressing the mTOR signalling by pharmacological inhibitors such as Metformin and Everolimus. This study was performed on different ovarian cancer cell lines, and also identified hypoxic microenvironment conditions as a catalyst for initiation of TNT formation. Hypoxia induces the cellular proliferation differently in chemoresistant (C200 and SKOV3) ovarian cancer cells and chemosensitive (A2780) cancer cells. Chemoresistant cellular model showed increased TNT formation and TNT mediated communication under hypoxic condition. Study found that chemoresistant SKOV3 cells showed increase in TNT numbers by 72 h with respect to normoxic conditions. This suggests TNTs might be involved in giving drug resistance to ovarian cancer cells .
Study published in 2013 further investigated the role of nanochannels between cancer and stromal cells in chemo-resistance cancer. They studied the TNTs formed in vitro between anchorage dependent spheroids as well as tumor explant cultures. In particular, they highlighted the transport of mitochondria through nanotubes between endothelial and cancer cells (ovarian and breast cancer cells) that corresponded with an acquired chemo-resistance in the recipient cancer cells . The study also described nanotubes formed between mesenchymal stem cells and cancer cells, with similar mitochondrial transfer. Thayanithy et al. also directly observed the intercellular transfer of miRNAs between cancer cells (osteosarcoma and ovarian cancer cell lines), including those that are implicated in conferring chemo-resistance . Figure 1 summarizes the different types of cell-cell communication mediated at nano-level interaction.
Viral hijacking of host cell TNTs
Membrane nanochannels have been directly observed to be present between a variety of cell types within our body. Recent research has also identified the key role played by nanochannels during viral infections [31,32,33]. Studies show that viral particles are capable of utilising the physical conduits between infected and non-infected cells in order to spread faster and infect a larger number of host cells. Viral transmission through intercellular conduits such as TNTs is one of the mechanism for virus particles to overcome the rate-limiting step of cell infection by diffusion through the matrix. Travelling through cells also ensures their survival by providing protection from antibodies and complement proteins in the extra-cellular serum. HTLV-1 virus escape from immune recognition by escaping through intercellular transmission . There can be several mode for viral transmission from cell-to-cell. Here, we want to highlight the fact based on few studies that cell-to-cell transmission through TNTs is one of the rate limiting step. In general, transmission through TNTs is one of the ways viruses escape from recognition by immune system . Study on gap junction (GJ) channels (Cx43) and TNTs showed that these two structures are important for efficient cell-to-cell communication and viral spread. Study also identified Cx43 protein localization in intracellular compartment at the base of the TNT. Study concluded that in macrophage Cx43 expresses and localized into the TNT in the presence of HIV infection . Another study found the presence of HIV particles within the TNTs and proposed that HIV highjacks the TNT communication for spreading from cell-to-cell . Despite of these evidences more researches are required in this area.
According to a study published in Nature Cell Biology , HIV transmission through physical intercellular connections is 100–1000 times more efficient than cell-free transmission. Membrane nanotubes between T cells are formed when they come in contact with one another, and the transmission of HIV viral particles through the T cell nanochannels was found to be dependent on CD4 and Env. HIV transmission was monitored through GFP-tagged Gag viral protein, and the rate of transmission through nanochannels was determined to be approximately 0.03 μm/s. This implies that TNTs are a major pathway for the transmission of HIV, and identifies them as a possible target for therapeutic strategies against HIV .
Another paper published also implicated the Env viral protein in playing a key role in the process of filopodial-bridge between an infected and an uninfected target cell . The viral envelope glycoprotein (Env) is involved in the recognition of receptors on uninfected target cells, following which virus moves along the outer surface of filopodial-bridge towards target cells . HIV infection in macrophages has also been observed to increase the initiation of nanotube formation, following which the viral particles hijack the nanotubes for efficient cell to cell transmission. The study also demonstrated that the formation of nanotubes corresponds with the time course of HIV viral replication .
In addition, Herpes Simplex Virus (HSV) was also observed to spread through thin intercellular cytoplasmic bridges up to 100 μm long, whose physical characteristics resemble TNTs. The study tagged an essential viral protein VP16 with a GFP tag and tracked the transmission of the viral particles through nanotubes, going from infected cells toward adjacent target uninfected host cells . A study on conserved alphaherpesvirinae proteins also revealed that the viruses in question are able to induce cytoskeletal rearrangements to form TNTs which they subsequently used for intercellular transmission. This viral subfamily includes the herpes simplex virus (HSV), varicella-zoster virus (VZV), porcine pseudorabies virus as well as other important animal virus that are responsible for a number of diseases. Intercellular viral spread was monitored in the presence and absence of nanochannel inhibitors and this pathway for transmission was determined to enhance the spread of the virus significantly .
A study in 2015 on Influenza A determined that the viral proteins could be transmitted through intercellular TNTs . Neuraminidase inhibitors failed to inhibit the viral spread to uninfected cells, however neuraminidase in conjunction with microtubule inhibiting drugs such as Paclitaxel had a significant impact on viral infection in vitro .
A recent study published in 2017 also highlights the role of TNTs in the spread of influenza virus. Lung epithelial cells are physically connected to each other through extensive nanochannel networks, which was exploited by the influenza virus for efficient cell-cell transmission . Confocal and scanning electron microscopy was also used to observe TNT formation before viral infection and viral transmission after infection. In the study, the viral transmission was not affected by neutralising antibodies or antiviral drugs and it was also determined that the networks could be used for complete viral genome and protein transmission . In the presence of neutralizing antibodies viral genome transfer through actin-based TNT structures . In the presence of antiviral drug, Zanamivir, genome transfer happens through TNTs from infected to naïve cells . Study using the inhibitor such as Nocodazole (microtubule inhibitor) found that TNT formation was attenuated in A549 cells. This also affected the cell-to-cell spread of viral genome . In Table 1 we have summarized the important development in this field.
TNTs mediate intercellular communication between immune cells
TNTs play a very big role in the cellular communication between a number of cell types in the human body, but nanochannel communications between cells of the immune system in particular have been widely studied in recent times [3, 24, 42, 43]. Nanotubular highways between different immune cells was first characterised by Önfelt et al. , and found TNTs between human peripheral blood NK cells, macrophages, and EBV-transformed B cells. According to the study, nanochannels can be created as immunological synapse move apart. They used single photon-excitation resonance scanning confocal microscopy to observe nanotubes in vitro between immune cells. Using GFP-tagged proteins, they also determined that cell surface proteins could be transferred to different cells through nanotubes over tens of microns and Class 1 MHC molecules were found to localise in these structures . Nanotubular networks can arise from transient intercellular contacts between immune cells. Time-lapse imaging showed the formation of long nanotube connecting the cells over 140 μm in peripheral blood NK cells and 721.21 transfectant cells expressing GFP-tagged HLA-C. Study found that average length of nanotube in all intercellular connection was 30 μm with dynamic change in length as cells moved apart and breakage of nanotube after few minutes (4 min). It has been found that in murine macrophage cells (J774) nanotube structure breaks after sometime which led to nanotubular connection between two cells only. According to the time-lapse experiment shown by Onfelt et al., class 1 MHC was seen on the nanotube which grew with a speed of around 0.2 μm/s and lasted for 15 min. These data suggests that class 1 MHC might be present on the tip of close-ended nanotubes. Another study in this line showed the transfer of class 1 MHC through TNTs in HeLa cells and blocking actin polymerization led to the reduced HLA-A2 transfer .
Multiple immune cells can be connected simultaneously by these TNTs, which in turn establishes a complex intercellular network between immune cells. The same group published another paper describing the two distinct types of nanotubes that were formed between macrophages based on their physical properties . Thin nanotubes were less than 0.7 μm in diameter, composed entirely of F-actin and bacteria could be trapped and transported within them, thick nanotubes were more than 0.7 μm in diameter and were comprised of both F-actin and microtubules. Thicker TNTs between macrophages were used by the cells for the transport of intracellular organelles such as mitochondria and lysosomes. It was also determined that transport through the nanochannel requires ATP as it was inhibited by the addition of azide . Active transport happens through TNTs and depletion of ATP leads to decreased intercellular transfer of vesicles . Transport of endocytic vesicles through TNTs are actomyosin-dependent. Study on PC12 and NRK cells showed that there is unidirectional movement of vesicles with a speed in the range of actin-dependent transport [3, 7]. Report shows that reduction in ATP production in mitochondria, activation of AMPK and inhibition of gluconeogenesis by metformin affects the TNTs . It has been shown that F-actin depolymerization drugs and depletion of ATP results in complete inhibition of organelle transfer [3, 24, 46].
Multiple studies also determined the relationship between the specific types of TNTs with directionality. Thin nanotubes containing only actin as the structural component were observed to have unidirectional transfer of intracellular cargo, whereas thicker nanotubes with both actin and microtubule elements as structural components were observed to have bi-directional transfer . Another study found nanotube bridges between cancerous monocytic cells, with as many as 75 TNTs per cell in areas with cells in close proximity . In monocytes and dendritic cells, the nanochannels were used to transport calcium fluxes instantly from cell to cell. In addition, GFP linked proteins were shown to be able to travel between B cells through nanochannels, showing that the plasma membrane between B cells are continuous. Myeloid cells therefore have been shown to form extensive TNTs for propagation of intercellular signals, however non-myeloid cells such as fibroblasts do not exhibit similar properties .
Another study determined the role of nanotubes in early events associated with BCR signal transduction. They demonstrated the dynamic nature of TNTs and their ability to move laterally on the surface of the cell, which had been also characterized by early experiments on nanochannels in cancer cells. They also identified CD45 and Syk as molecular markers for induction of TNT formation in B cells as evidenced by their inducible co-localisation with the nanotubes . A 2001 study also observed the formation of TNTs in human neutrophils in vitro when cultured in specific conditions, specifically requiring Na + free medium which inhibited the neutrophils from spreading. Thick nanochannels had unattached tips radiating out from the cell body, which grew up to 70 μm in just 20 mins. Upon addition of Na + ions, spreading was restored and nanochannel formation was reduced. TNT formation in neutrophils was observed to be an alternative mechanism for cell adhesion and communication in the study . Figure 2 summarizes the cell-to-cell interaction mediated by nanochannel.
Endothelial cells form TNTs for intercellular transport
TNTs are also known to play a significant role in maintaining the function of endothelial cells along with their surrounding tissues. Endothelial cells that are present in the extra-cellular matrix surrounding tumor tissue was found to form extensive TNTs with the tumor cells, and transfer of organelles between the different cell types modulate chemo-resistance in tumor cells, as well as endothelial phenotype [22, 30]. In a recent 2015 study , TNTs were found between microvascular endothelial cells (HMECs) that transported organelles such as mitochondria and lysosomes, as well as lipid droplets through membrane connections through distances as long as over 100 μm. VEGF treatment was seen to significantly increase the number of lipid droplets, while arachidonic acid not only increased lipid droplet transport but also induced a 3-fold increase in TNT formation .
Yasuda et al. also discovered the effect of direct mitochondrial and lysosomal transfer between endothelial cells through TNTs and identified it as the primary driving force behind robust functional improvement in vasculopathies beyond endothelial progenitor cells . Endothelial progenitor cells (EPCs) were observed to form extensive TNT connections with stressed endothelial cells, and subsequent lysosomal transfer not only improved endothelial cell viability, but also reduced premature senescence and apoptosis. Human umbilical vein endothelial cells (HUVECs) were used in the study along with EPCs, and there was observed to be a 3 fold increase in organelle transfer when the HUVEC cells were exposed to increased concentrations of cytotoxic drugs, indicating TNTs to be a specific pathway for repair . Another study on the neuroprotective properties of neural stem cells (NSCs) also demonstrated a key role of TNT formation between NSCs and the brain microvascular endothelial cells (BMECs). Stressed BMECs from sites of ischemic injury were observed to induce TNT formation in surrounding NSCs in in vitro conditions. The TNTs were composed of both F-actin and microtubules, and electron microscopy was used to demonstrate mitochondrial exchange . M-Sec, a marker for TNT formation, was observed to enhance the formation of TNTs between cells when up-regulated and was proposed as a possible therapeutic target for ischemic stroke injuries . M-Sec is shown to be the marker for TNT and play important role in its formation. M-sec can induce the formation of TNTs in co-operation with RalA small GTPase and the exocyst complex which suggest the role actin remodeling in TNTs formation . M-Sec gene expression is induced by TNF-alpha. Retinoic acid is also shown to induce this gene expression. Overexpression of TNFAIP2 (M-Sec) reverses the inflammatory response and miR-221 induced apoptosis. RNAi based therapy can be utilized for targeting TNT structure. Other than RNAi based approach, actin inhibitor such as Cytochalasin can be utilized for targeting TNT formation. Drugs such as Nocodozole (Microtubule) can be used for inhibiting growth of TNTs.
TNTs are one of the most novel methods of mechanical intercellular communication over long distances, and research over the last two decades has demonstrated TNTs to be increasingly ubiquitous among different cell types. TNTs are used by cells for intercellular communication through calcium fluxes, proteins, miRNAs and organelles such as mitochondria, endosomes, and lysosomes. These components are transported through nanochannels along F-actin or microtubules by motor proteins, and hence require ATP. TNTs between immune cells, endothelial cells and stem cells are used to reverse stress and diseased conditions through intercellular mitochondrial and lysosomal transfer [52, 53]. TNTs also play a very important role in diseased conditions such as cancer. Nanotubes aid tumor cells in proliferation, as well as migration and initiation of metastasis [1, 22]. TNTs between cancer cells and cells from the surrounding extra-cellular matrix facilitate transfer oncogenic mRNAs to healthy cells . TNTs between cancer cells also allows them to rapidly modulate the chemo-resistance of their neighbouring tumor cells [26, 28, 30]. All of these functions are affected when cells were treated with drugs that inhibit TNTs. Cytoskeletal inhibitors such as Latrunculin A, Cytochalasin D, Docetaxal , Jasplakinolide  as well as mTOR signalling inhibitors such as Metformin and Everolimus  are used as pharmacological inhibitors for nanotube formation. Markers such as M-Sec can also be used for targeted therapies . Proteins that are involved in receptor-dependent HIV transmission between immune cells such as Gag , Env and CD4  are also effective targets for peptide or pharmacological inhibitors. The cellular signals and machinery involved in TNT formation and maintenance in disease conditions can therefore be specifically targeted for novel therapies for cancers, as well as viral diseases such as AIDS [2, 37], Herpes , chickenpox (varicella) and porcine pseudorabies . Cellular machinery such as actin remodeling, inflammatory signals and microtubule organization can be targeted to inhibit the formation of TNTs. Targeting molecules such as M-sec along with miR221 can be manipulated to target the formation of TNTs. As cancer cells secretes several inflammatory cytokines such as TNF-alpha and TNF-alpha is one of the important factor associated with M-sec gene expression, inhibiting expression of TNF-alpha can block the formation of TNTs and hence intercellular communication between cancer cells. Viruses escape the recognition by immune cells by spreading through TNTs. As the immunological synapse involved in TNT formation, TNT growth can be targeted by targeting these synapse. Combination therapies for Influenza both targeting TNT formation and neuraminidase have already been established as effective strategies in preclinical trials.
With the growing knowledge about TNTs, it is now well known that they are involved in different functions which includes cellular communication to disease progression. However, due to their recent discovery, there are many aspects which still need to be explored. As discussed previously, TNT’s are formed through actin driven de novo process, however, there are certain alternative mechanism also through which they are formed. Detailed knowledge about these alternative and de novo mechanisms which includes “what triggers them to form” especially in case of cancer cells can proved to be useful from therapy point of view. Additionally, they are known to be involved in microbial and viral infection. Detailed overview of all the receptors present on them can provide much deeper insight to the mechanism of disease progression. Further, therapeutic resistance which has been linked with TNT’s can also be explored thoroughly in order to circumvent it. One of the major challenge in this field is presence of different types of TNTs which are cell specific. Transcriptomics and proteomics based studies can further give us more insight about these nano-level interaction. Finally, there is need to explore the role of TNTs in connection with reactive oxygen species (ROS) which may further help in finding their role in complex diseases such as diabetes, Alzheimer’s and Parkinson’s.
B cell receptor
Cluster of differentiation 4
Cluster of differentiation 45
Endothelial progenitor cell
Green fluorescent protein
Human immunodeficiency virus
Major histocompatibility complex
Mechanistic target of rapamycin
- NK cells:
Natural Killer cells
Scanning electron microscope
Spleen associated tyrosine kinase
Transmission electron microscope
Vascular endothelial growth factor
Lou E, Fujisawa S, Morozov A, Barlas A, Romin Y, Dogan Y, Gholami S, Moreira AL, Manova-Todorova K, Moore MA. Tunneling nanotubes provide a unique conduit for intercellular transfer of cellular contents in human malignant pleural mesothelioma. PLoS One. 2012;7:e33093.
Sowinski S, Jolly C, Berninghausen O, Purbhoo MA, Chauveau A, Köhler K, Oddos S, Eissmann P, Brodsky FM, Hopkins C. Membrane nanotubes physically connect T cells over long distances presenting a novel route for HIV-1 transmission. Nature cell biol. 2008;10:211.
Gurke S, Barroso JF, Gerdes H-H. The art of cellular communication: tunneling nanotubes bridge the divide. Histochem Cell Biol. 2008;129:539–50.
Abounit S, Zurzolo C. Wiring through tunneling nanotubes–from electrical signals to organelle transfer. J Cell Sci. 2012;jcs:083279.
Gerdes H-H, Carvalho RN. Intercellular transfer mediated by tunneling nanotubes. Curr Opin Cell Biol. 2008;20:470–5.
Eugenin EA, Gaskill PJ, Berman JW. Tunneling nanotubes (TNT) a potential mechanism for intercellular trafficking. Commun Integr Biol. 2009;2:243–4.
Rustom A, Saffrich R, Markovic I, Walther P, Gerdes H-H. Nanotubular highways for intercellular organelle transport. Science. 2004;303:1007–10.
Gerdes H-H. Prions tunnel between cells. Nature Cell Biol. 2009;11:235.
Gousset K, Schiff E, Langevin C, Marijanovic Z, Caputo A, Browman DT, Chenouard N, De Chaumont F, Martino A, Enninga J. Prions hijack tunnelling nanotubes for intercellular spread. Nature Cell Biol. 2009;11:328.
Xu W, Santini PA, Sullivan JS, He B, Shan M, Ball SC, Dyer WB, Ketas TJ, Chadburn A, Cohen-Gould L. HIV-1 evades virus-specific IgG2 and IgA responses by targeting systemic and intestinal B cells via long-range intercellular conduits. Nature Immunol. 2009;10:1008.
Rudnicka D, Schwartz O. Intrusive HIV-1-infected cells. Nature Immunol. 2009;10:933.
Arkwright PD, Luchetti F, Tour J, Roberts C, Ayub R, Morales AP, Rodríguez JJ, Gilmore A, Canonico B, Papa S. Fas stimulation of T lymphocytes promotes rapid intercellular exchange of death signals via membrane nanotubes. Cell Res. 2010;20:72.
Watkins SC, Salter RD. Functional connectivity between immune cells mediated by tunneling nanotubules. Immunity. 2005;23:309–18.
Vidulescu C, Clejan S, O'Connor KC. Vesicle traffic through intercellular bridges in DU 145 human prostate cancer cells. J Cell Mol Med. 2004;8:388–96.
Eugenin E, Gaskill P, Berman J. Tunneling nanotubes (TNT) are induced by HIV-infection of macrophages: a potential mechanism for intercellular HIV trafficking. Cellular Immunol. 2009;254:142–8.
Lou E, Fujisawa S, Barlas A, Romin Y, Manova-Todorova K, Moore MA, Subramanian S. Tunneling nanotubes: a new paradigm for studying intercellular communication and therapeutics in cancer. Commun Integr Biol. 2012;5:399–403.
Zhang L, Zhang Y. Tunneling nanotubes between rat primary astrocytes and C6 glioma cells alter proliferation potential of glioma cells. Neurosci Bull. 2015;31:371–8.
Rustom A. The missing link: does tunnelling nanotube-based supercellularity provide a new understanding of chronic and lifestyle diseases? Open Biol. 2016;6:160057.
Lou E, O'hare P, Subramanian S, Steer CJ. Lost in translation: applying 2D intercellular communication via tunneling nanotubes in cell culture to physiologically relevant 3D microenvironemnts. FEBS J. 2017;5:699–707.
Castro MA, Grieneisen VA, de Almeida RM. Disruption and de novo formation of nanotubular membrane extensions in SW620 colon carcinoma cell line during cell division. Cell Biol Int. 2005;29:929–31.
Pontes B, Viana NB, Campanati L, Farina M, Neto VM, Nussenzveig HM. Structure and elastic properties of tunneling nanotubes. Eur Biophys J. 2008;37:121–9.
Connor Y, Tekleab S, Nandakumar S, Walls C, Tekleab Y, Husain A, Gadish O, Sabbisetti V, Kaushik S, Sehrawat S. Physical nanoscale conduit-mediated communication between tumour cells and the endothelium modulates endothelial phenotype. Nat Commun. 2015;6:8671.
Wang X, Bukoreshtliev NV, Gerdes H-H. Developing neurons form transient nanotubes facilitating electrical coupling and calcium signaling with distant astrocytes. PLoS One. 2012;7:e47429.
Onfelt B, Nedvetzki S, Benninger RK, Purbhoo MA, Sowinski S, Hume AN, Seabra MC, Neil MA, French PM, Davis DM. Structurally distinct membrane nanotubes between human macrophages support long-distance vesicular traffic or surfing of bacteria. J Immunol. 2006;177:8476–83.
Austefjord MW, Gerdes H-H, Wang X. Tunneling nanotubes: diversity in morphology and structure. Commun Integr Biol. 2014;7:e27934.
Thayanithy V, Dickson EL, Steer C, Subramanian S, Lou E. Tumor-stromal cross talk: direct cell-to-cell transfer of oncogenic microRNAs via tunneling nanotubes. Transl Res. 2014;164:359–65.
Thayanithy V, Babatunde V, Dickson EL, Wong P, Oh S, Ke X, Barlas A, Fujisawa S, Romin Y, Moreira AL. Tumor exosomes induce tunneling nanotubes in lipid raft-enriched regions of human mesothelioma cells. Exp Cell Res. 2014;323:178–88.
Frankfurt OS, Seckinger D, Sugarbaker EV. Intercellular transfer of drug resistance. Cancer Res. 1991;51:1190–5.
Desir S, Dickson EL, Vogel RI, Thayanithy V, Wong P, Teoh D, Geller MA, Steer CJ, Subramanian S, Lou E. Tunneling nanotube formation is stimulated by hypoxia in ovarian cancer cells. Oncotarget. 2016;7:43150.
Pasquier J, Guerrouahen BS, Al Thawadi H, Ghiabi P, Maleki M, Abu-Kaoud N, Jacob A, Mirshahi M, Galas L, Rafii S. Preferential transfer of mitochondria from endothelial to cancer cells through tunneling nanotubes modulates chemoresistance. J Transl Med. 2013;11:94.
Hashimoto M, Bhuyan F, Hiyoshi M, Noyori O, Nasser H, Miyazaki M, Saito T, Kondoh Y, Osada H, Kimura S. Potential role of the formation of tunneling nanotubes in HIV-1 spread in macrophages. J Immunol. 2016. https://doi.org/10.4049/jimmunol.1500845.
Panasiuk M, Rychłowski M, Derewońko N, Bieńkowska-Szewczyk K. Tunneling nanotubes (TNT) as a novel route of cell-to-cell spread of herpesviruses. J Virol. 2018;JVI:00090–18.
Kumar A, Kim JH, Ranjan P, Metcalfe MG, Cao W, Mishina M, Gangappa S, Guo Z, Boyden ES, Zaki S. Influenza virus exploits tunneling nanotubes for cell-to-cell spread. Sci Rep. 2017;7:40360.
Omsland M, Pise-Masison C, Fujikawa D, Galli V, Fenizia C, Parks RW, Gjertsen BT, Franchini G, Andresen V. Inhibition of tunneling nanotube (TNT) formation and human T-cell leukemia virus type 1 (HTLV-1) transmission by Cytarabine. Sci Rep. 2018;8:11118.
Okafo G, Prevedel L, Eugenin E. Tunneling nanotubes (TNT) mediate long-range gap junctional communication: implications for HIV cell to cell spread. Sci Rep. 2017;7:16660.
Mothes W, Sherer NM, Jin J, Zhong P. Virus cell-to-cell transmission. J Virol. 2010;84:8360–8.
Sherer NM, Lehmann MJ, Jimenez-Soto LF, Horensavitz C, Pypaert M, Mothes W. Retroviruses can establish filopodial bridges for efficient cell-to-cell transmission. Nature Cell Biol. 2007;9:310.
La Boissiere S, Izeta A, Malcomber S, O'Hare P. Compartmentalization of VP16 in cells infected with recombinant herpes simplex virus expressing VP16-green fluorescent protein fusion proteins. J Virol. 2004;78:8002–14.
Favoreel HW, Van Minnebruggen G, Adriaensen D, Nauwynck HJ. Cytoskeletal rearrangements and cell extensions induced by the US3 kinase of an alphaherpesvirus are associated with enhanced spread. Proc Natl Acad Sci U S A. 2005;102:8990–5.
Roberts KL, Manicassamy B, Lamb RA. Influenza a virus uses intercellular connections to spread to neighboring cells. J Virol. 2015;89:1537–49.
Roberts KL, Manicassamy B, Lamb RA. Influenza a virus uses intercellular connections to spread to neighboring cells. J Virol. 2014;JVI:03306–14.
McCoy-Simandle K, Hanna SJ, Cox D. Exosomes and nanotubes: control of immune cell communication. Int J Biochem Cell Biol. 2016;71:44–54.
Tarakanov AO, Goncharova LB. Cell-cell nanotubes: tunneling through several types of synapses. Commun Integr Biol. 2009;2:359–61.
Onfelt B, Nedvetzki S, Yanagi K, Davis DM. Cutting edge: membrane nanotubes connect immune cells. J Immunol. 2004;173:1511–3.
Schiller C, Huber JE, Diakopoulos KN, Weiss EH. Tunneling nanotubes enable intercellular transfer of MHC class I molecules. Hum Immunol. 2013;74:412–6.
Bukoreshtliev NV, Wang X, Hodneland E, Gurke S, Barroso JF, Gerdes H-H. Selective block of tunneling nanotube (TNT) formation inhibits intercellular organelle transfer between PC12 cells. FEBS Lett. 2009;583:1481–8.
Marzo L, Gousset K, Zurzolo C. Multifaceted roles of tunneling nanotubes in intercellular communication. Front Physiol. 2012;3:72.
Salter RD, Watkins SC. Dynamic properties of antigen uptake and communication between dendritic cells. Immunol Res. 2006;36:211–20.
Gupta N, DeFranco AL. Visualizing lipid raft dynamics and early signaling events during antigen receptor-mediated B-lymphocyte activation. Mol Biol Cell. 2003;14:432–44.
Galkina SI, Sud'ina GF, Ullrich V. Inhibition of neutrophil spreading during adhesion to fibronectin reveals formation of long tubulovesicular cell extensions (cytonemes). Exp Cell Res. 2001;266:222–8.
Astanina K, Koch M, Jüngst C, Zumbusch A, Kiemer AK. Lipid droplets as a novel cargo of tunnelling nanotubes in endothelial cells. Sci Rep. 2015;5:11453.
Yasuda K, Khandare A, Burianovskyy L, Maruyama S, Zhang F, Nasjletti A, Goligorsky MS. Tunneling nanotubes mediate rescue of prematurely senescent endothelial cells by endothelial progenitors: exchange of lysosomal pool. Aging (Albany NY). 2011;3:597.
Wang X, Yu X, Xie C, Tan Z, Tian Q, Zhu D, Liu M, Guan Y. Rescue of brain function using tunneling nanotubes between neural stem cells and brain microvascular endothelial cells. Mol Neurobiol. 2016;53:2480–8.
Ohno H, Hase K, Kimura S. M-sec: emerging secrets of tunneling nanotube formation. Commun Integr Biol. 2010;3:231–3.
Yamashita YM, Inaba M, Buszczak M. Specialized intercellular communications via Cytonemes and nanotubes. Annu Rev Cell Dev Biol. 2018;34:59–84.
Dupont M, Souriant S, Lugo-Villarino G, Maridonneau-Parini I, Vérollet C. Tunneling nanotubes: intimate communication between myeloid cells. Front Immunol. 2018;9:43.
Önfelt B, Purbhoo MA, Nedvetzki S, Sowinski S, Davis DM. Long-distance calls between cells connected by tunneling nanotubules. Sci STKE. 2005;2005:pe55.
Spees JL, Olson SD, Whitney MJ, Prockop DJ. Mitochondrial transfer between cells can rescue aerobic respiration. Proc Natl Acad Sci U S A. 2006;103:1283–8.
Davis DM. Intercellular transfer of cell-surface proteins is common and can affect many stages of an immune response. Nature Rev Immunol. 2007;7:238.
Guo R, Davis D, Fang Y. Intercellular transfer of mitochondria rescues virus-induced cell death but facilitates cell-to-cell spreading of porcine reproductive and respiratory syndrome virus. Virology. 2018;517:122–34.
Cifuentes-Muñoz N, Dutch RE, Cattaneo R. Direct cell-to-cell transmission of respiratory viruses: the fast lanes. PLoS Pathog. 2018;14:e1007015.
Valdebenito S, Lou E, Baldoni J, Okafo G, Eugenin E. The novel roles of Connexin channels and tunneling nanotubes in Cancer pathogenesis. Int J Mol Sci. 2018;19. https://doi.org/10.3390/ijms19051270.
Jia L, Shi Y, Wen Y, Li W, Feng J, Chen C. The roles of TNFAIP 2 in cancers and infectious diseases. J Cell Mol Med. 2018. https://doi.org/10.1111/jcmm.13822.
Seema Sehrawat is the recipient of BioCARe Award from Department of Biotechnology, Ministry of Science and Technology, Govt. of India. Shiv Nadar Foundation is acknowledged for providing the PhD fellowship to Mr. Naveen Kumar and Mr. Peeyush Prasad. Taruna Sharma is supported by CSIR, India. Aaron Goldman is supported by the Breast Cancer Alliance Young Investigator Award.
BioCARe Award from Department of Biotechnology, Ministry of Science and Technology, Govt. of India (Recipient: Seema Sehrawat).
Breast Cancer Alliance Young Investigator Award (Recipient: Aaron Goldman).
Shiv Nadar Foundation: Providing fellowship to Peeyush Prasad and Naveen Kumar. CSIR, India- Providing fellowship to Taruna Sharma
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Jash, E., Prasad, P., Kumar, N. et al. Perspective on nanochannels as cellular mediators in different disease conditions. Cell Commun Signal 16, 76 (2018). https://doi.org/10.1186/s12964-018-0281-7