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Fig. 4 | Cell Communication and Signaling

Fig. 4

From: CaGdt1 plays a compensatory role for the calcium pump CaPmr1 in the regulation of calcium signaling and cell wall integrity signaling in Candida albicans

Fig. 4

Activation of the CaMkc1- and CaCek1-mediated MAP kinase signaling in cells lacking CaGDT1 and/or CaPMR1. Phosphorylation levels of CaMkc1, CaCek1 and their C-terminally HA-tagged versions were determined simultaneously by Western blotting using the Phospho-p44/42 MAPK Rabbit mAb in protein extracts prepared from log-phase growing cells of the wild-type (WT) CAI4, the WJCAG22 (gdt1/gdt1), the NGY98 (pmr1/pmr1) and the WJCAG25 (pmr1/pmr1 gdt1/gdt1) strains, in which either the CaMKC1 allele (Panel a) or the CaCEK1 allele (Panel b) was chromosomally HA-tagged, in the presence or absence of 50 μg ml− 1 Calcofluor white (CFW). The anti-HA antibody (Abmart, Shanghai, China) was used to reveal the total amount of CaMkc1-HA and CaCek1-HA proteins loaded. Each lane was loaded with 50 μg of total proteins

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Cell Communication and Signaling

ISSN: 1478-811X