The arabidopsis cyclic nucleotide interactome

Table 1 Arabidopsis cyclic nucleotide binding protein candidates

		LEAF cAMP				LEAF cGMP				CALLUS cAMP				CALLUS cGMP
Accession	Description	2-AHA-cAMP-agarose	8-AEA-cAMP-agarose	2-[Biotin]-AHA-cAMP	cAMP antibody	2-AH-cGMP-agarose	8-AET-cGMP-agarose	2-[Biotin]-AH-cGMP	cGMP antibody	2-AHA-cAMP-agarose	8-AEA-cAMP-agarose	2-[Biotin]-AHA-cAMP	cAMP antibody	2-AH-cGMP-agarose	8-AET-cGMP-agarose	2-[Biotin]-AH-cGMP	cGMP antibody
AT3G13920	eukaryotic translation initiation factor 4A1			B		B			B	B	B	B	B	B	B	B	B
AT3G12780	phosphoglycerate kinase 1	B	B	B	B	B	B	B	B
AT1G42970	glyceraldehyde-3-phosphate dehydrogenase B subunit	B	B	B	2, 3, B	B	B	B	B
AT4G20360	RAB GTPase homolog E1B	B	B	B	2, 3, B	B	B	B	B
AT3G60750	Transketolase		B	B	B	B	B	B	B
AT5G50920	CLPC homologue 1	B	B	B	B	B	B	B
AT4G37930	serine transhydroxymethyltransferase 1	2, B	B	1, B	B	B	B	2, 3, B
AT3G14420	Glycolate oxidase 1		B		B			B	B
AT4G09650	ATP synthase delta subunit			B		B		B	B
AT1G09340	chloroplast stem-loop binding protein of 41 kDA			1	B	B		2, B
AT1G56330 AT3G62560 AT4G02080	secretion-associated RAS 1B									B					B		B
AT4G27440	protochlorophyllide oxidoreductase B		B		B	B
AT3G01500	carbonic anhydrase 1			B		B

The fraction in which the protein was identified is indicated with numbers 1–6 being the elution fractions (1 denotes the first and least stringent elution and 6 the final and most stringent elution) while B indicates that the protein was found in the bead fraction. For the cAMP baits the elutions were 1) 100 mM GDP; 2) 100 mM AMP; 3) 10 mM cGMP; 4) 100 mM cGMP; 5) 10 mM cAMP and 6) 100 mM cAMP. For the cGMP baits the elutions were 1) 100 mM ADP; 2) 100 mM GMP; 3) 10 mM cAMP; 4) 100 mM cAMP; 5) 10 mM cGMP and 6) 100 mM cGMP

ISSN: 1478-811X