Figure 6
From: Stoichiometry and intracellular fate of TRIM-containing TCR complexes
Differential processing of TRIM compared to other αβTCR complex components. (A) Human peripheral blood T cells were incubated with anti-CD3ε mAb 2Ad2a2 to induce cap formation for 1 and 23 hours or left unstimulated. Cells were fixed and the subcellular localization of TRIM detected using biotinylated TRIM-4 mAb and Texas Red-coupled streptavidin; LAMP1 was stained with FITC-labelled anti-Lamp 1 antibody. (B) Cells were unstimulated (top) or stimulated as above for 18 hours. In all panels, actin was visualized using a Cy2-labelled goat anti-rabbit antiserum. Primary mAb antibodies were: OKT3 for CD3ε; 6B10.2 for ζ; TRIM-7 for TRIM; and W6/32 for HLA-1. Texas Red-labelled donkey anti-mouse was used as the secondary antibody.