Fig. 5
From: Atg5 deficiency in macrophages protects against kidney fibrosis via the CCR6-CCL20 axis
Macrophage autophagy-related 5 (Atg5) deletion attenuated macrophage renal infiltration in vivo. Bone marrow-derived monocytes (BMDMs) were isolated from WT and MΦ atg5-/- mice, labeled with the tracking dye 5-chloromethylfluorescein diacetate (CMFDA), and injected into WT mice; unilateral acute kidney injury was induced the next day. The labeled cells in the kidney were evaluated 3 days after ischemic injury. (A) Representative image of injured kidneys and the number of CMFDA and F4/80 double-positive renal-infiltrated cells in mice receiving Atg5 knockout (KO) and WT monocytes. Scale bar: 50 μm. Data are presented as the mean ± SEM. ** P < 0.01 (n = 4 per group). (B) Flow cytometric analysis to determine the F4/80 and CMFDA double-positive cell numbers in mice receiving Atg5 KO and WT monocytes. Data are presented as the mean ± SEM. ** P < 0.01 (n = 6 per group). Unpaired, two-tailed Student’s t test