Fig. 1
From: Atg5 deficiency in macrophages protects against kidney fibrosis via the CCR6-CCL20 axis
Renal myeloid autophagy-related 5 (Atg5) was efficiently deleted in LysM-Cre+/−Atg5f/f (MΦ atg5−/−) mice. (A) Breeding protocol for generating LysM-Cre+/−Atg5f/f (MΦ atg5−/−) and Cre−/−Atg5f/f (WT) male littermates, aged 8–10 weeks, for subsequent experiments after genotypes were confirmed. (B) MΦ atg5−/− mice verified by polymerase chain reaction using isolated genomic DNA. (C) Atg5 mRNA levels in immunomagnetically sorted kidney macrophages from WT and MΦ atg5−/− mice. Data are presented as the mean ± SEM, n = 3 per group, **** P < 0.0001 versus WT macrophages. Unpaired, two-tailed Student’s t test