Fig. 6
From: PAX1 represses canonical Wnt signaling pathway and plays dual roles during endoderm differentiation
PAX1 represses Wnt signaling pathway in foregut and pharyngeal endoderm cells. A-B qRT-PCR analyses of AFE and PE associated genes in hESC-derived AFE (A) and PE (B) cells. AFE: anterior foregut endoderm; PE: pharyngeal endoderm. PAX1 OE: PAX1 overexpression. C-D qRT-PCR analyses performed on known Wnt target genes in hESC-derived AFE (C) and PE (D) cells. E Western blotting to analyze the protein levels of indicated proteins in WT and PAX1 OE hESC-derived PE cells. ß-actin was used as loading control