Fig. 6

p66Shc mediated autophagy through cGAS/STING pathway A-C qRT-PCR analysis of relative cytosolic mtDNA levels in A 5 mM or 30 mM glucose treated HTR8/SVneo cells, B Control and p66WT overexpressed HTR8/SVneo cells, C Control and p66Shc-KD cells. *P < 0.05. D Western blot images of protein levels of cGAS, p-STING and STING in normal and GDM placentae. E Western blot images and quantifications of protein levels of cGAS, p-STING and STING in 5 mM or 30 mM glucose treated HTR8/SVneo cells. **P < 0.01. F Western blot images and quantifications of protein levels of cGAS, p-STING and STING in control and p66Shc-KD cells. *P < 0.05. G Western blot images and quantifications of protein levels of p-STING, STING and LC3II in HTR8/SVneo cells treated with 2’3’-cGAMP and Chloroquine. **P < 0.01, ***P < 0.001, ****P < 0.0001. H Western blot images and quantifications of protein levels of STING, LC3 and ATG5 in control and STING-KO HTR8/SVneo cells. *P < 0.05. I Co-immunoprecipitation coupled western blot images demonstrate the interactions between STING and LC3 or ATG5 in both control and p66Shc-KD cells