Fig. 6

MSC-derived mitochondria-mediated DSBs are close to the CTCF binding site. To investigate the positional correlation between mitochondria-mediated double-strand breaks (DSBs) and CTCF binding sites, dorsal root ganglions (DRGs) were isolated from sham and SNI with mitochrondrial injection therapy (SNI + Mito) groups, and γH2AX ChIP-seq as well as immunoprecipitation were employed. (A) UCSC genome browser views denoting the disposition of γH2AX signals at Atf3 under the sham and SNI with mitochrondrial injection therapy (SNI + Mito). (B) The plot denotes the disposition of input-normalized γ-H2AX signals relative to CTCF sites that displayed γ-H2AX peaks in their vicinity. The dashed line denotes the profile of γ-H2AX in the sham group, whereas the solid line indicates γ-H2AX profiles in the SNI with mitochrondrial injection therapy (SNI + Mito) group. (C) DRGs from the sham and SNI + Mito groups were lysed and γ-H2AX was immunoprecipitated. The precipitates were analyzed by western blotting with the γ-H2AX and CTCF antibodies