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Fig. 5 | Cell Communication and Signaling

Fig. 5

From: MSC-derived mitochondria promote axonal regeneration via Atf3 gene up-regulation by ROS induced DNA double strand breaks at transcription initiation region

Fig. 5

MSC-derived mitochondrial transfer mediates DSBs at the Atf3 gene transcription initiation region. To detect double-strand breaks (DSBs) in various regions of the Atf3 gene, the ChIP-PCR assay was employed, utilizing γ-H2AX and 53BP1 antibodies along with primers targeting the Atf3 promoter, exon 2, and 3’UTR. (A-C) ChIP-PCR analysis of γ-H2AX binding to the Atf3 promoter (A), exon 2 (B) and 3’UTR (C) regions in the sham, SNI and SNI with mitochrondrial injection therapy (SNI + Mito) groups. (D-F) ChIP-PCR analysis of 53BP1 binding to the Atf3 promoter (D), exon 2 (E) and 3’UTR (F) regions in the sham, SNI and SNI + Mito groups

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Cell Communication and Signaling

ISSN: 1478-811X