Fig. 4
Effects of Gdf9 variants on fertility and follicular development. A Average number of pups produced per cage (each cage contains 2 females and 1 male) by females Gdf9wt/wt (n=3), Gdf9Q308X/Q308X (n=3), Gdf9S415T/S415T (n=3) and Gdf9Q308X/S415T (n=3) when paired with wild-type males. *P < 0.05. B Average litter sizes of Gdf9wt/wt n=9) and Gdf9S415T/S415T (n=8) when paired with wild-type males. *P < 0.01. C Appearance of wild-type and variant ovaries as viewed through the stereoscope. D H&E staining of wild-type and mutant ovaries. E Follicle counts and follicle counts per area (mm2) of Gdf9wt/wt (n=3), Gdf9S415T/S415T (n=3) and Gdf9Q308X/S415T (n=3) mice (PrF: Primordial follicles, PF: Primary follicle, SF: Secondary follicle, AF: Antral follicle, AtF: Atretic follicle). *P < 0.05