Fig. 9
From: DEC1 is involved in circadian rhythm disruption-exacerbated pulmonary fibrosis
The cell cycle is rhythmical, and bleomycin disrupts the cell cycle rhythm in alveolar epithelial cells. (A) The website predicted the rhythms of circadian clock proteins and related cell cycle protein (Circadian Expression Profiles Data Base: http://circadb.hogeneschlab.org/). (B) The rhythms of the mRNA levels of circadian clock genes and cell cycle genes were detected in C57 mouse lung tissue by qRT-PCR. n = 6. (C, D) After treat RLE-6TN cells with bleomycin, Western blot analysis was performed to detect the rhythmic change of related proteins, and statistical analysis of blots was conducted. n = 3. (E) Schematic illustration of the binding sites between DEC1 and p21 promoter regions. (F) DNA electrophoresis gels showed PCR products obtained after the reaction with ChIP-enriched DNA. (G) ChIP-qPCR was used to quantitatively detect the binding of DEC1 to the p21 promoter region. n = 4. *P < 0.05. P values were determined by the one-way ANOVA followed by the Bonferroni’s test