Fig. 8
From: DEC1 is involved in circadian rhythm disruption-exacerbated pulmonary fibrosis
Knockdown of DEC1 with DEC1 siRNA attenuated bleomycin or TGF-β1 induced ATII cell senescencein vitro. (A) RLE-6TN cells were treated with bleomycin and DEC1 siRNA, and the expression levels of senescence-associated proteins DEC1, p53, p21, and CDK2 were detected by Western blot analysis. (B) Statistical analysis of the Western blot bands corresponding to the senescence-associated proteins detected. DEC1: n = 12, p53: n = 8, p21: n = 6, and CDK2: n = 3. (C, D) After treating RLE-6TN cells with bleomycin or TGF-β1 with or without DEC1 siRNA, cellular senescence was detected by staining with β-galactosidase. (E, F) Statistical analysis of senescent cells in according to C and D, n = 3. (G, H) SASP (IL-1α and IL-6) in epithelial cells was detected by qRT-PCR after treating RLE-6TN cells with bleomycin with or without DEC1 siRNA (G: n = 4, H: n = 6). (I, J) RLE-6TN cells were treated with bleomycin or TGF-β1 with or without DEC1 siRNA, and changes in the cell cycle were detected by flow cytometry, n = 3. *P < 0.05, **P < 0.01, ***P < 0.001, ns means nonsignificant. P values were determined by the one-way ANOVA followed by the Bonferroni’s test