Fig. 5
From: DEC1 is involved in circadian rhythm disruption-exacerbated pulmonary fibrosis
Mouse type II alveolar epithelial cell specific knockout of DEC1 attenuated bleomycin-induced mouse pulmonary fibrosis. (A) Representative images of DEC1 gene knockout in mouse alveolar epithelial cells were verified by immunofluorescence. Original magnification, ×400. (B) DEC1 conditional knockout mice were used to construct a pulmonary fibrosis model. The figure showed CT scan and 3D reconstruction images of mouse lung. (C) Masson staining of lung tissues. Original magnification, ×200. (D) Sirius red staining of lung tissues. Original magnification, ×200. (E, F) Representative blots showing the expression level of related proteins in lung tissue of mice, detected by Western blot analysis (n = 6). *P < 0.05, **P < 0.01, ***P < 0.001. P values were determined by the one-way ANOVA followed by the Bonferroni’s test