Fig. 4
From: DEC1 is involved in circadian rhythm disruption-exacerbated pulmonary fibrosis
DEC1-siRNA suppressed bleomycin-induced mouse pulmonary fibrosis. C57BL/6J mice were subjected to intraperitoneal injection of DEC1 siRNA lentivirus to knock down DEC1 gene, and then a pulmonary fibrosis model was established by intraperitoneal injection of bleomycin. (A) Efficiency validation of DEC1 interference in mouse lung tissue (n = 5). (B) A panoramic and 400-times magnified representative images of Masson staining of mouse lung tissue. (C) Quantitative analysis of collagen deposition area in mouse lung tissue (n = 6). (D) Sirius red staining of mouse lung tissues. (E) Micro-CT scan image and 3D reconstruction of mouse lung. (F) Western blot analysis of fibronectin, collagen-I, and α-SMA in mouse lung tissue (n = 6), quantitative analysis of fibronectin, collagen-I, and α-SMA protein blots. *P < 0.05, **P < 0.01, ***P < 0.001. P values were determined by the one-way ANOVA followed by the Bonferroni’s test