From: Interleukin-4 protects retinal ganglion cells and promotes axon regeneration
Chemicals | Source | Identifier | Dosage |
---|---|---|---|
Staurosporine | Abmole (Beijing, China) | Cat# M2066 | Â |
N-methyl-D-aspartic Acid (NMDA) | Abmole | Cat# M2884 | Â |
4’,6-diamidino-2-phenylindole (DAPI) | Solarbio (Beijing, China) | Cat# C0065 |  |
Antifading mounting medium | Solarbio | Cat# S2100 | Â |
Cholera Toxin Subunit B (CTB) conjugated Alexa Fluor 488 | Thermo Fisher Scientific (MA, USA) | Cat# C34775 | Â |
recombinant murine IL-4 protein | PeproTech (Rocky Hill, NJ, USA) | Cat# 214 − 14 |  |
Reagents for cell histology | Source | Identifier | Dosage |
mouse anti-βIII-Tubulin | Beyotime (Shanghai, China) | Cat# AT809 | 1:100 |
rabbit anti-NeuN | Abcam (Cambridge, UK) | Cat# ab177487 | 1:400 |
DyLight 488, anti-mouse IgG | Abbkine (Shanghai, China) | Cat# A23210 | 1:400 |
Cy3, anti-rabbit IgG | Abbkine | Cat# A22220 | 1:400 |
Reagents for Western blot | Source | Identifier | Dosage |
mouse anti-β-III Tubulin | Beyotime | Cat# AT809 | 1:2000 |
rabbit anti-NeuN | Abcam | Cat# ab177487 | 1:5000 |
mouse anti-β-actin | Signalway (Pearland, TX, USA) | Cat# 21,800 | 1:8000 |
anti-rabbit IgG, HRP conjugated | Signalway | Cat# L3012 | 1:8000 |
anti-mouse IgG, HRP conjugated | Signalway | Cat# L3032 | 1:8000 |
Reagents for tissue histology | Source | Identifier | Dosage |
rabbit anti-RBPMS | OmnimAbs (California, USA) | Cat# OM165217 | 1:200 |
rabbit anti-GAP43 | Beyotime | Cat# AF0153 | 1:200 |
mouse anti-β-III Tubulin | Beyotime | Cat# AT809 | 1:100 |
rabbit anti-IL4R | Affinity Biosciences | Cat# DF8567 | 1:200 |
DyLight 488, anti-rabbit IgG | Abbkine | Cat# A23220 | 1:400 |
DyLight 488, anti-mouse IgG | Abbkine | Cat# A23210 | 1:400 |
Cy3, anti-rabbit IgG | Abbkine | Cat# A22220 | 1:400 |