Fig. 9
From: Interleukin-4 protects retinal ganglion cells and promotes axon regeneration
RGC axon regeneration after ONC injury induced by recombinant IL-4 protein. A: CTB fluorescence images and GAP43 immunofluorescence staining images of axons distal to the injury site. In the rIL-4 group, there were axons labeled with both CTB (green) and GAP43 (red) distal to the injury site on days 7 and 14. The length of double-labeled axons distal to the injury site was longer in the rIL-4 treatment group on day 14 than on day 7. B, C: The number of CTB-labeled axons beyond the injury site. The number of regenerated axons with lengths greater than 500 μm beyond the injury site in the rIL-4 group was significantly higher than that on day 7 in the rIL-4 group and that on day 14 in the vehicle group. D, E: The number of axons beyond the injury site traced by GAP43 immunofluorescence staining. The number of regenerated axons with lengths greater than 500 μm beyond the injury site in the rIL-4 group was significantly higher than that on day 7 in the rIL-4 group and that on day 14 in the vehicle group. n = 5 nerves per group, * indicates p < 0.05, ** indicates p < 0.01, **** indicates p < 0.001