Fig. 6
From: Interleukin-4 protects retinal ganglion cells and promotes axon regeneration
Recombinant IL-4 protein attenuated NMDA-induced axon degeneration and maintained long-range axon projections in RGCs. A, B: Immunofluorescence images of retinal ganglion cell axons traced by CTB-Alexa Fluor 488. The average relative fluorescence intensity in the rIL-4 group was higher than that in the vehicle group on days 7 and 14. C, D: The cross-sectional area where axons were quantified was 1 cm distal to the injury site. The number of axons labeled with CTB was counted in five areas on a cross section of the optic nerve. Each area was 0.02 × 0.02 (mm2). The mean number of CTB-labeled axons in an area was calculated. E, F: On day 7, the mean axon density labeled by CTB in the rIL-4 group was greater than that in the vehicle group. G, H: CTB labeling in the lateral geniculate body and superior colliculus of mice. On day 7, the relative fluorescence intensity of CTB in the LGN and SC in the contralateral side of rIL-4 treated eyes was significantly higher than that in the contralateral side of vehicle treated eyes. n = 4 nerves or brains, *** indicates p < 0.005, **** indicates p < 0.001