Fig. 3
From: Interleukin-4 protects retinal ganglion cells and promotes axon regeneration
Induction of axon outgrowth by recombinant IL-4 protein in 661 W cells. A: Light microscopy image of 661 W cells treated with vehicle and 1 µg/mL rIL-4 for 24 h. Axon-like structures (indicated by red arrows) extended from 661 W cells in the rIL-4 group. B: Immunofluorescence staining of βIII-tubulin showed that the axon-like structure extended from 661 W cells in the rIL-4 group was βIII-tubulin positive. C: Neurite tracing showed that the neurite extension length of the rIL-4 group was significantly longer than that of the vehicle group at the same treatment time. D, E, F: The fluorescence intensity of NeuN (red) and βIII-tubulin (green) in the rIL-4 group was higher than that in the vehicle group. Western blot results showed that the protein levels of βIII-tubulin and NeuN in the rIL-4 group were higher than those in the vehicle group. n = 3 per group, * indicates p < 0.05, *** indicates p < 0.005