Fig. 1
From: Interleukin-4 protects retinal ganglion cells and promotes axon regeneration
Induction of axon outgrowth in 661 W cells by STS. A: Light microscopy showed that 661 W cells treated with 50 nM STS for 6 h extended long axon-like structures (indicated by red arrows). B: The extended axon-like structures of 661 W cells treated with 50 nM STS showed positive immunofluorescence staining for βIII-tubulin. C: Neurite tracing showed that the extended neurites of 661 W cells treated with 50 nM STS were longer than those treated with vehicle. D, E: Higher fluorescence intensity of NeuN (red) and βIII-tubulin (green) was observed in 661 W cells treated with STS. F: Western blot results showed that the protein levels of NeuN and βIII-tubulin were higher in 661 W cells treated with STS. n = 3 per group, ** indicates p < 0.01, *** indicates p < 0.005, **** indicates p < 0.001