Fig. 1
MoCsn5 is required for vegetative growth and conidiation. A Colony morphology of 70-15, ΔMocsn5 and Mocsn5-C. The strains were grown on CM and MM plates for 9 days. B Hyphal growth of 70-15, ΔMocsn5 and Mocsn5-C on solid CM. C Statistical analysis of the colony growth diameter. The data were analyzed using GraphPad Prism 8.0 software. The error bars represent the standard deviations. ***P < 0.001. D Conidiophores of 70-15, ΔMocsn5 and Mocsn5-C. The strains were cultivated in an incubator at 25 ℃ for 9 days and observed under an optical microscope. E Statistical analysis of conidia production. The data were analyzed with GraphPad Prism 8.0 software. ***P < 0.001. F Localization of MoCsn5 in the conidium, appressorium and invasive hypha. The MoH2B-mCherry vector was transformed into the complementation strain with the MoCsn5-GFP label. Conidia were harvested from colonies that were cultivated in solid CM for 9 days. The conidial suspension (5 × 104 ml−1) was dripped onto a hydrophobic film and incubated in a humid chamber at 22 °C for 24 h. The red fluorescence and green fluorescence in the spores and appressoria were observed under a fluorescence microscope. Bar: 10 μm. G The fluorescence densities of MoH2B-mCherry and MoCsn5-GFP were analyzed using ImageJ software