Fig. 5

Knockdown of PR130 sensitizes PDAC cells to cytotoxic effects of phendione. a MIA PaCA-2 cells were transfected twice within 48 h with 30 nM of the indicated siRNAs (sicon, control siRNAs; siPR130, siRNAs against PR130). After two days, cells were incubated with 3 µM phendione for 48 h. Cell death analysis was done by flow cytometry using annexin-V/PI staining. Data are shown as mean values ± SD; n = 3. Data were statistically analyzed using two-way ANOVA (* p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001). b siRNA transfection was performed as described in (a). Cells were treated with 3 µM phendione for another 24 h and immunoblot was done to detect PR130 and HSP70. HSP90 served as loading control; n = 4. c Quantification of HSP70 expression and normalization to the loading control of 4 independent immunoblots from (b). d siRNA transfections were performed as described in (a); sicon, control siRNAs; siPR130, siRNAs against PR130. After transfection, MIA PaCA-2 cells were treated with 3 µM phendione (PD) and collected after 24 h and 48 h; con, untreated control cells. Data were analyzed by using the aggresome detection kit and were measured by flow cytometry; n = 3. e Quantification of data from (c). Data were statistically analyzed using one-way ANOVA (* p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001)