Fig. 5
From: Kinesin KIFC3 is essential for microtubule stability and cytokinesis in oocyte meiosis
KIFC3 affects midbody formation and cytokinesis in mouse oocytes (A) Spindle morphology at the ATI stage and MII stage oocytes. Green, α-tubulin; blue, DNA. Repeat the experiment 4 times. Bar = 20 μm. (B) Co-IP was performed with an anti-KIFC3 antibody. The immunoblots of protein precipitates were probed with anti-PRC1 antibody. (C) Control and KIFC3-antibody injection oocytes at the ATI stage were stained with anti-α-tubulin (green), anti-PRC1 (red) and counterstained with Hoechst 33342 to visualize the chromosomes (blue). Repeat the experiment 3 times. Bar = 20 μm. (D) Diagram of the roles of KIFC3 during mouse oocyte meiotic maturation. KIFC3 regulated tubulin acetylation to stabilize the microtubules for spindle assembly and kinetochore-microtubules attachment, and KIFC3 also interacted with PRC1 for cytokinesis during mouse oocyte meiosis