Fig. 3

KIFC3 regulates kinetochore-microtubule attachment in mouse oocytes (A) Control and KIFC3-antibody injection oocytes at the MI stage were stained with anti-α-tubulin (green), anti-CREST (red) and counterstained with Hoechst 33342 to visualize the chromosomes (blue). Bar = 10 μm. (B) The rate of abnormal kinetochore-microtubule attachment after KIFC3-antibody injection at the MI stage. Graph shows means ± sem of results obtained in 3 independent experiments. *P < 0.05. (C) Control and KIFC3-antibody injection oocytes at the MI stage were stained with anti-H3S10ph (red) and counterstained with Hoechst 33342 to visualize the chromosomes (blue). Bar = 5 μm. (D) The scattergram shows the relative fluorescence intensity of H3S10ph signals in control and KIFC3-antibody injection oocytes. Graph shows means ± sem of results obtained in 3 independent experiments. ***P < 0.001. (E) Control and KIFC3-antibody injection oocytes at the MI stage were stained with anti-BubR1 (green) and counterstained with Hoechst 33342 to visualize the chromosomes (blue). Bar = 5 μm. (F) The histogram shows the percentages of control and KIFC3-antibody injection oocytes with abnormal BubR1. Graph shows means ± sem of results obtained in 3 independent experiments. *P < 0.05. (G) Co-IP was performed with an anti-KIFC3 antibody. The immunoblots of protein precipitates were probed with an anti-Bub3 antibody. (H) Control and KIFC3-antibody injection oocytes at the MI stage were stained with anti-Bub3 (red) and counterstained with Hoechst 33342 to visualize the chromosomes (blue). Bar = 5 μm. (I) The histogram shows the percentages of control and KIFC3-antibody injection oocytes with abnormal Bub3. Graph shows means ± sem of results obtained in 3 independent experiments. **P < 0.01