Fig. 2
From: Kinesin KIFC3 is essential for microtubule stability and cytokinesis in oocyte meiosis
KIFC3 regulates spindle formation and chromosome alignment in mouse oocyte meiosis (A) Control and KIFC3-antibody injection oocytes at the MI stage were stained with anti-α-tubulin (green) and counterstained with Hoechst 33,342 to visualize the chromosomes (blue). Bar = 20 μm. (B) The rate of abnormal spindle morphology and misaligned chromosome after KIFC3-antibody injection at the MI stage. Graph shows means ± sem of results obtained in 3 independent experiments. *P < 0.05. (C) Thickness of the spindle middle plate. C indicates the maximal span of chromosomes; S indicates the maximal spindle length. Bar = 10 μm. The scattergram shows the C: S ratios for control and KIFC3-antibody injection oocytes at the MI stage. Graph shows means ± sem of results obtained in 3 independent experiments. ***P < 0.001. (D) Immunofluorescence analysis of γ-tubulin (red) and α-tubulin (green) in control and KIFC3-antibody injection oocytes. Bar = 10 μm. The histogram shows the percentages of control and KIFC3-antibody injection oocytes with abnormal γ-tubulin. Graph shows means ± sem of results obtained in 3 independent experiments. *P < 0.05. (E) Immunofluorescence analysis of p-Aurka (red) and α-tubulin (green) in control and KIFC3-antibody injection oocytes. Bar = 10 μm. The histogram shows the percentages of control and KIFC3-antibody injection oocytes with abnormal p-Aurka. Graph shows means ± sem of results obtained in 3 independent experiments. *P < 0.05