Fig. 7

Selective binding of NFκB monomers to the genes of Il10 and Il12a were explored by Chromatin Immunoprecipitation studies. a Pictorial representation of p50 and p65 binding to their corresponding TFBMs on mouse Il10 gene, due to NLGP transduced signal, followed by obstructions towards Dectin-1. TFBM sequences identified by sequence alignment are shown (distance mentioned from the transcription start site or TSS) (above) along with agarose gel images of relative amplifications of protein-bound TFBM (below) (TFBM stands for transcription factor binding motif). b Data from different replicates (n = 3) are represented as mean ± SD. c Data for comparative binding of p65 and p50 to Il12a gene TFBMs from replicates (n = 3), as mean ± SD. d Binding of p65 and p50 to Il12a TFBMs, represented pictorially in the same way as for Il10 in (a). Numerical data of (b) and (c) were tested by two-way ANOVA, followed by Tukey’s multiple comparisons test. Normal distribution of the data were verified before each test (See also Fig. S3)