Fig. 6

Presence of NLGP within the cytosol alongside its accumulation by the membrane of mouse dendritic cells, reveals its intracellularization following the interaction(s) with Dectin-1 receptors. a A 2D fluorescence-micrograph of mBMDCs incubated with NLGP-FITC for 1 h, captured through confocal microscope (nuclei stained with DAPI). b Sum slices type 2D Z-projection of the field, derived by flattening the Z-stack (individual images of successive layers of the Z-stack are shown in Additional file 5, sequentially from above along a specific thickness interval) through sum slices method. c Maximum intensity type 2D Z-projection of the field (scale bars:10 µm in (a), (b) and (c)). d Surface view of the field (cells’ exterior surfaces shown in blue/cyan) (See also Additional file 6). e FITC channel-based volume view of the field (See also Additional file 7). f Longitudinal section was obtained through the composite view (surface view + volume view) of the cells, along white dots. Left side of this section was rotated clockwise around the Y-axis or the vertical axis revealing cell-interiors along section. g Rotated composite view, cut parallel to Y–Z plane. h Longitudinal section through volume view of the field, rotated to reveal cell interiors. i Rotated volume view cut parallel to Y–Z plane (See also Additional file 8). j Rotated composite view of the field (as in (g)), positioned horizontally (upper) and an area along the section, revealing the cell interiors is marked. The marked zone is magnified further (lower) revealing NLGP-FITC within the cells. k Slice (orthoslice) obtained by rotating the volume view, cut parallel to Y–Z plane (as in (i)), placed horizontally (upper), revealing intracellularized NLGP-FITC, within the magnified marked region (lower)