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Fig. 1 | Cell Communication and Signaling

Fig. 1

From: Neem leaf glycoprotein binding to Dectin-1 receptors on dendritic cell induces type-1 immunity through CARD9 mediated intracellular signal to NFκB

Fig. 1

The water-soluble component of Azadirachta indica, called Neem leaf glycoprotein or NLGP has a polypeptide-based foundation. a Chromatogram of NLGP from high-performance liquid chromatography (AU stands for absorbance units, in 210 nm). b Absorbance spectrum of NLGP from wavelengths of 200 nm to 1000 nm, compared to that of phosphate buffered saline. c Electrophoresis of NLGP in polyacrylamide gels. d LC/MS views of the fragment ion hits after MS2 of tandem mass spectrometry of the protein-moiety of NLGP, with (upper) and without (middle) relative intensities. Formation of fragment ion-peaks in a magnified LC/MS view (lower) (RT stands for retention time, m/z is the mass to charge ratio). e Primary mass spectrogram of precursor ions (NLGP-peptides) after MS1 through data independent acquisition. f Deduction of a peptide spectrum (secondary mass spectrogram) from b- and y- fragment ion-hits (da stands for daltons)

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Cell Communication and Signaling

ISSN: 1478-811X