Fig. 8
From: Tumor-derived miR-6794-5p enhances cancer growth by promoting M2 macrophage polarization
miR-6794-5p increases metastasis by inducing M2 polarization in vivo. A-B C57BL/6 mice were injected via tail vein with negative control (NC), miR-6794-5p, or miR-6794-5p + SOCS1 overexpressing LLC1 cells (n = 5; 5 X 105 cells/mouse). Lung tissue was harvested by sacrifice at 4 weeks after cell injection. A Lung tissue images of each group were subjected to H&E and IHC staining with anti-SOCS1. Scale bar is 100 μm. B IHC staining was performed with anti-CD206 using the lung tissues of each group. Scale bars are 100 μm (top) and 50 μm (bottom). C-F Negative control (NC) and miR-6794-5p overexpressed LLC1 cells were subcutaneously injected into the right flank of C57BL/6 mice (n = 4; 2 X 105 cells/mouse). After tumorigenesis, the expression levels of M1 macrophages, M2 macrophages and activated CD8+ T cells in the tumor tissues of the two groups were analyzed by representative flow cytometry. C, D Respective percentages of M1 macrophages (CD45+ F4/80+ CD11b + MHCII+ CD206- cells) and M2 macrophages (CD45+ F4/80+ CD11b + MHCII- CD206+ cells) isolated from tumor tissues developed in negative control (NC) and miR-6794-5p overexpressing mice were analyzed by flow cytometry. E The M1/M2 ratio was calculated based on the percentages of M1 and M2 macrophages analyzed by flow cytometry. F The proportion of activated CD8+ T cells (CD45+ CD8+ CD3+ CD25+ cells) isolated from tumor tissue developed in negative control (NC) and miR-6794-5p overexpressing mice was analyzed by flow cytometry. G Schematic diagram illustrating the mechanism of exosomal miR-6794-5p secreted from tumor cells on surrounding macrophages