Fig. 6
From: Tumor-derived miR-6794-5p enhances cancer growth by promoting M2 macrophage polarization
Targeting of IL-10 in M2 macrophages prevents tumor malignancy. A-E After THP-1 derived macrophages were transfected with miR-6794-5p mimics or siRNA against IL-10 (si IL-10), the indicated cells were subjected to qRT-PCR (A), ELISA (B), Western blot (C), wound healing (D), and matrigel coated invasion (E) assays. Scale bar is 200 μm. The values were normalized to GAPDH in qRT-PCR. β-actin was used for normalization in Western blot analysis. The experiment was repeated with triplicates and representative Western blotting images are shown. The data are presented as the mean ± S.D. after triplicate. *P < 0.05; ** P < 0.01; ***P < 0.001. One-way ANOVA followed by bonferroni comparison test